Table of Contents
Surgical Embryo Transfer Goat
Exposure of uterus by midventral laparotomy
Small incision made in lower portion of uterine
horn with a scalpel
Blunt large gauge needle is used to enlarge hole
into uterine horn
End of Tomcat catheter is pushed into the uterine
lumen
Bulb of Tomcat catheter is expanded with air to
seal the end of the uterine horn
A small hole is made with blunt end of a surgical
needle to insert flushing media
Needle is pushed into uterine lumen at the tip
of the uterine horn near the utero-tubal junction
Once flushing needle is in place, the syringe with
flushing media is attached
Approxiatmently 30-40 ml of flushing media is pushed
through the uterine horn toward the base of the uterus
Care is taken to ensure slow even flow of media
through the catheter
Media is collected into a grided Petri dish to
assist in locating embryos
Embryos are washed several times in holding media
before transfer or freezing
Embryos are place in freezing media containing
0.1% sucrose and ethylene glycol
Embryos are placed in straws and frozen in liquid
nitrogen
Freezing is done in a programmable freezer that
controls small changes in temperature and time |