Table of Contents
Milk Progesterone ELISA
Why run milk progesterone Assay
Collect Milk Sample
Label Cow Sample with I.D.
Accufirm Progesterone Kit
Prepare Standard Sample - A
Addition of Standard - Progesterone Only to Tube
A
Squeeze Rack and Pour Off Fluid
Starting with First Milk Tube at Left - Add Progesterone
-Enzyme Complex
Shake Rack for 10 Seconds Wait 1 minute to Proceed
Pour of Fluid
PPT Slide
Remove Excess Water by Shaking Rack
Add Enzyme Substrate (C) to Tubes Starting with
Milk Tubes
Add Two Drops of Developer (D) to All Tubes
Color Develops Read Samples Between 1 and 2 Minutes
Darker Blue Compared to Standard Means Low Progesterone
in Sample
Samples can be Read with RTR Reader
Comparison of Standard - Tube A With Milk Tube
- S
Reading Indicates High or Low Progesterone |