Animal Science Faculty

Randall S. Prather

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Curators' Professor

Reproductive Physiology and Molecular Biology, Transgenic Pigs

Animal Science Research Center
Division Of Animal Sciences
Columbia, MO 65211

• Phone: 573-882-6414/6391
• Fax: 573-884-7827
• Email
• Curriculum Vitae

Career Objective

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To improve reproductive efficiency in domestic animals by performing directed research in developmental biology and reproductive physiology, by focusing on the molecular, cellular and physiological events surrounding ovulation, fertilization and preimplantation embryonic development.

Education

Training and Research Experience

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• September ’06-Present; Curators’ Professor, University of Missouri System.
• November ’04-Present; Associate Leader, Food for the 21st Century Reproductive Biology Cluster, University of Missouri-Columbia.
• September ’03-Present; Co-Director, National Swine Research and Resource Center, University of Missouri-Columbia.
• September ’01-Present; Distinguished Professor of Reproductive Biotechnology, University of Missouri-Columbia.
• September ‘99-August ’01; Professor, Department of Animal Sciences, University of Missouri-Columbia.
• September ‘95-August ‘99; Associate Professor, Department of Animal Sciences, University of Missouri-Columbia.
• June ‘89-August ‘95; Assistant Professor, Department of Animal Sciences, University of Missouri-Columbia.
• June ‘88-June ‘89; Postdoctoral Research Assistant, Dept. Meat & Animal Science, University of Wisconsin-Madison.
• March ‘87-June ‘88; Postdoctoral Research Assistant, Dept. Zoology, Dept. of Meat & Animal Science, University of Wisconsin-Madison.
• June ‘84-February ‘87; Research Assistant, Department of Meat and Animal Science, University of Wisconsin-Madison.
• June ‘82-May ‘84; Temporary Assistant Instructor, Department of Surgery and Medicine, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas.
• January ‘82; Basic Milker's School, Kansas State University, Manhattan, Kansas.
• ‘75-’84; Farm Manager and Worker, Osage Farms Inc., Garnett, Kansas.

Research Interests

1. Mechanisms of Oocyte Activation
2. Transcriptional Regulation of Differentiation
3. In Vitro Production of Embryos
4. Nuclear Reprogramming After Nuclear Transfer

5. Transgenic Animal Production
6. Genomic Studies in Reproduction

Web Sites (a few)

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http://swine.rnet.missouri.edu/
http://bovine.rnet.missouri.edu/
http://genome.rnet.missouri.edu/Ovine/
http://nsrrc.missouri.edu/
http://cafnr.missouri.edu
http://www.npr.org/templates/story/story.php?storyId=5300300
http://sympaticomsn.ctv.ca/servlet/ArticleNews/story/CTVNews/20060324/omega_pig_060324?sym=rss#
http://sciencenow.sciencemag.org/cgi/content/full/2006/327/
http://www.newswise.com/articles/2002/1/KNOCKOUT.UMC.html
http://www.pbs.org/newshour/bb/science/jan-june97/cloning_2-24.html
http://www.junkscience.com/news/wilmut3.htm
http://www.cnn.com/2002/HEALTH/01/03/pig.cloning/index.html
http://www.missouri.edu/~news/releases/octnov01/Pratherpigs.html
http://www.nytimes.com/2006/03/26/health/26cnd-pig.html
http://www.ctv.ca/servlet/an/story/CTVNews/20060324/omega_pig_060324
http://www.cbn.com/cbnnews/healthscience/060330a.aspx

Professional Membership

• American Association for the Advancement of Science ‘89-Present.
• Society of Developmental Biologists ‘86-Present.
• Society for the Study of Reproduction ‘84-Present.
• International Embryo Transfer Society ‘83-Present.
• American Society of Animal Scientists ‘83,’94-‘98.
• Society for the Study of Fertility ‘94-‘99.
• American Scientific Affiliation ‘86-’91.
  Sigma Xi ‘87-’96.

Awards/Honors

• Faculty-Alumni Award. The University of Missouri. Columbia, MO October 10, 2008.
• Research Award, Society for the Study of Reproduction. Kailua-Kona, Hawaii May 27, 2008.
• 21st Century Corps of Discovery Lecture. University of Missouri- Chancellor’s Award. 2007
• Discover Magazine Top 100 Science Stories in 2006. Our Omega-3 pigs ranked number 38.
• President’s Award for Research and Creativity, University of Missouri System. May 4, 2006.
• American Physiology Society, Walter Randall Award for Biomedical Ethics, April 4, 2006. San Francisco, CA.
• National Geographic Magazine, Photo and story on transgenic pigs expressing the enhanced green fluorescent protein. July 2005, page 18.
• Presidential Faculty Entrepreneur of the Year Award, University of Missouri System. March 5, 2003.
• Animal Molecular and Cell Biology Distinguished Lecturer, University of Florida. May 3, ’03.
• Popular Science’s Medical Tech Grand Award Winner. The best of what’s new- Knockout pigs- The most significant advance in medical technology for the year 2002.
• Discover Magazine Top 100 Science Stories in 2002. Our alpha 1,3 galactosyltransferase knock-out pigs ranked number 36.
• Missouri’s Excellence in Life Science Award for Excellence in Life Science Research for 2002. Missouri Biotechnology Association (MOBIO), November 14, 2002.
• Distinguished Professor of Reproductive Biotechnology, September 1, ’01, University of Missouri-Columbia.
• Outstanding Research Award, American Society of Animal Science- Midwestern Section, March 19,’97.
• Researcher of the Year, Junior Faculty, College of Agriculture, Food & Natural Resources, University of Missouri, October 1, ‘96.
• Junior Faculty Research Award, Gamma Sigma Delta University of Missouri, March 27, ‘96.
• Finalist-Student Competition, International Embryo Transfer Society; ‘84, ‘85, ‘86.
• Counselor, Kansas Boy’s State, Lawrence, KS. ’78.
• Delegate, Kansas Boy’s State, Lawrence, KS, ’77.

Miscellaneous

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Swine in Biomedical Research- Organization Committee. San Diego, April 2-3, ‘08.

International Embryo Transfer Society- Chaired a session entitled ‘Societal Needs’ at the annual meeting. January 5-9, ’08.

International Organizing Committee- 8th International Conference on Pig Reproduction. Treasurer. June ’05-‘09. Banff, Canada.

Swine in Biomedical Research- Organization Committee- Meeting held in Chicago in January 2005.

USDA/NRICGP Reproductive Biology of Animals - Grant Review Panel May 31-April 4, ’03. Washington D.C.

USDA/NRICGP Reproductive Biology of Animals - Grant Review Panel May 13-16, ’02. Washington D.C.

Abstract Session Chair. International Embryo Transfer Society. Co-Chaired the review of 65 abstracts on cloning with Brett White at the University of Nebraska. Fall ’01.

International Organizing Committee- 7th International Conference on Pig Reproduction. Treasurer. June ’00-‘05. The Netherlands.

International Organizing Committee- 6th International Conference on Pig Reproduction. June 3-6, ‘01. Columbia, MO.

Editorial Board- Xenotransplantation. Blackwell Publishing. May ’02- Present.

Executive Editor- Molecular Reproduction & Development. Wiley-Liss, Inc. March ’07 - Present.

Associate Editor- Molecular Reproduction & Development. Wiley-Liss, Inc. November ’99-February ‘07.

Board of Editors- Cloning, now re-titled Cloning & Stem Cells. Mary Ann Liebert, Inc. Publishers. September ‘98- Present.

Editorial Advisory Board- Animal Reproduction Science. Elsevier Science. January ‘00- December ‘01.

Organizing Committee- Nuclear Transfer and Transgenesis. June 18-21, ‘00. Park City, Utah.

Chairman-Session on: Stem Cells. Nuclear Transfer and Embryonic Stem Cells. April 15-17, ‘98. Monash University, Australia.

Physiology Committee - Midwest Section of the American Association of Animal Scientists/ American Dairy Science Association, ‘96-‘98. Chairman ‘97-‘98.

Research Awards Committee- Midwest Section of the American Association of Animal Scientists/ American Dairy Science Association, ‘97-‘00.

Co-Chair- Session on: Embryo transfer in pigs. Annual Meeting of the International Embryo Transfer Society, Salt Lake City, Utah, January 8, ‘96.

Chairman- Session on: Activation of the oocyte. Annual Meeting of the International Embryo Transfer Society, Salt Lake City, Utah, January 9, ‘96.

Director - Transgenic Animal Facility, in the Animal Sciences Research Center, ‘90-‘94.

USDA/NRICGP Reproductive Biology of Animals - Grant Review Panel June 1-4, ‘93. Washington D.C.

Local Organizing Committee - 4th International Conference on Pig Reproduction, Columbia, MO, May 23-26, ‘93.

Local Organizing Committee – 22nd Annual Meeting of the Society for the Study of Reproduction, Columbia, MO, Aug. 6-9, ‘89.

Education Committee, International Embryo Transfer Society ‘91-‘92, ‘97-‘99.

Ad Hoc Reviewer

Journals

- Animal Biotechnology; ’04, ’05, ’06, ‘07;
- Animal Genetics; ’95, ‘07;
- American Journal of Transplantation, ’03;
- Animal Reproduction Science, ‘97, ‘98, ’99, ’02, ’03, ’04, ’05, ’06, ’07, ‘08;
- Biology of Reproduction; ‘88, ‘89, ‘90, ‘91, ‘92, ‘93, ‘94, ‘95, ‘96, ‘97, ‘98, ’99, ’00, ’01, ’02, ’03, ’04, ’05, ’06, ’07, ‘08;
- Developmental Biology; ‘89, ‘90, ‘91, ‘92, ‘93, ‘94, ’95, ’00, ‘06;
- Developmental Dynamics; ’06;
- Differentiation; ‘95;
- DNA and Cell Biology; ’03;
- Domestic Animal Endocrinology; ’99;
- Gamete Research; ‘89;
- Genome; ’00;
- Journal of Animal Science; ‘91, ‘94, ‘95, ’98, ‘06;
- Journal of Experimental Zoology; ‘91, ‘92, ‘93, ‘94;
- Journal of Reproduction & Fertility; ‘89, ‘90, ‘91, ‘92, ‘95, ‘97, ’98, ’99 ‘00;
- Microscopy Research and Technique; ‘99;
- Molecular Reproduction & Development; ‘89, ‘90, ‘91, ‘92, ‘93, ‘94, ’95, ‘96, ‘97, ‘98, ‘99;
- Nature Reviews Genetics; ’00;
- Nature Biotechnology; ’01, ’05, ’08;
- Proceedings of the National Academy of Science; ‘96, ’97, ’00, ’01, ’03, ’04, ’05, ’07, ‘08;
- Reproduction; ’00, ’01, ’05, ’06, ’07, ‘08;
- Reproduction, Fertility and Development; ‘97, ‘98 ’99, ’00, ’01, ’02, ’03, ’04, ’05, ‘08;
- Reproduction Nutrition Development; ‘95;
- Science; ’00, ‘01;
- Theriogenology; ‘89, ‘90, ‘91, ‘92, ‘93, ‘94, ‘95, ‘96, ‘97, ‘98, ’99, ’00, ’01, ’02, ’03, ’04, ’06, ‘07;
- Transgenic Research; ’05, ’06, ‘07;
- Transplantation; ’06;
- Xenotransplantation; ’01, ’02, ‘06;
- Zygote; ‘97, ‘98.

Grant Proposals

- Austrian Science Fund; ‘97, ‘98;
- Biotechnology and Biological Sciences Research Council, UK, ‘97, ‘98;
- Global Research Network Program- Korea Research Foundation, ’08.
- International Foundation for Science; ‘97;
- Lincoln University AES-Jefferson City Mo; ’99;
- Massachusetts Agricultural Experiment Station; ‘95;
- Medical Research Council of Canada; ‘93;
- Missouri Agricultural Experiment Station (Lincoln University); ‘97;
- National Institutes of Health, NIAID, Innovation grant program: approaches in HIV vaccine research. ‘98;
- National Institutes of Health, NICHD, REB. ‘99; NICHD, ZHD1, ’00; NINDS, NSD-B, ’02;
- National Science Foundation; ’94, ‘06;
- Natural Sciences and Engineering Research Council of Canada; ’93, ‘08;
- North Carolina Biotechnology Center; ‘97;
- The Israel Science Foundation; ’99, ‘02;
- Ohio Cancer Research Associates; ‘97;
- USDA CSREES Small Business Innovation Research Program; ‘90, ‘91, ’98, ’99, ‘02;
- USDA CSRS CRGO Animal Growth & Development; ‘90;
- USDA CSRS CRGO Animal Science - Reproductive Physiology; ‘90;
- USDA CSRS NRI CGP Animal Science - Reproductive Biology of Animals; ‘91, ‘92, ‘95, ‘96, ’97, ’99, ’01, ’04, ‘05;
- USDA CSRS NRI CGP Animal Science - Animal Molecular Genetics; ‘92, ’99, ‘00;
- USDA CSRS NRI CGP Animal Science - Cellular Growth...; ‘92, ‘95, ‘96;
- USDA CSRS NRI CGP Improving Animal Growth; ‘98;
- USDA CSREES Biotechnology Risk Assessment; ’04;
- University of Wisconsin Sea Grant College Program; ‘97.
Team Captain - Kansas State University, Animal Science and Industry Academic Quadrathalon. 2nd place team, ‘82.

FUNDING (Total Costs)

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As Principal Investigator or Co-Investigator (Subcontracts as PI/PD)

2009-2011; National Institutes of Health, R21. Large animal model of spinal muscle atrophy. M.A. Lorson, PI, C.L. Lorson, R.S. Prather, K. D. Wells, Co-PIs. $ 411,125.
2007-2008. National Institutes of Health. R13. Swine in Biomedical Research. Conference Grant. L. Riley, J. Critser & R.S. Prather, PI’s. $ 30,000.
2008-2013. National Institutes of Health. U42. Competitive Renewal of the National Swine Resource and Research Center. L. Riley, J. Critser & R.S. Prather, PI’s. $8,274,384.
2007-2008. Hematech, Inc. Production of pigs/fetuses for Hematech. R.S. Prather, PI. $ 40,000
2007-2008. United States Department of Agriculture. NRI. Swine in Biomedical Research. Conference Grant. L. Riley, J. Critser & R.S. Prather, PI’s. $ 10,000
2007-2011. National Institutes of Health. Production of transgenic pigs by nuclear transplantation. Competitive renewal of R01. R.S. Prather, PI, Gordon Springer, Co-PI. $1,162,175
2007-2010. The Discovery Eye Foundation. Functional reconstruction of the outer retina. Michael Young, PI, Harvard; Henry Klassen, UC-Irvine; Robert S. Langer, MIT; Erik Scherfig, University of Copenhagen-Denmark; Karin Warfvinge, Lund University, Sweden; Erin Lavik, Yale University. Henry Kaplan, University of Louisville, KY. Subcontract to the University of Missouri, R. S. Prather, PI. $99,190. Total funding $5,989,793.
2006-2009. United States Department of Agriculture. ARS. 1 year. Program for the prevention of animal infectious diseases and advanced technologies for vaccines and diagnostics. Knockout of sialoadhesin in swine. Direct costs only. R.S. Prather, PD. $ 244,000
2006-2009. United States Department of Agriculture. NRI. 2 Years. Development of Defined Conditions for Pig Embryo Culture. R.S. Prather, PD. $ 284,153
2005-2008. United States Department of Agriculture. Development of ovine expressed sequence tags for the study of female reproduction. J.A. Green, R.S. Prather, D.H. Keisler, M.F. Smith, G.K. Springer, J.F. Taylor, C. Elsik PDs. $ 560,629
2005. United States Department of Agriculture. ARS. 7th International Conference on Pig Reproduction. Direct costs only. R.S. Prather, PD. $ 2,500
2005-2009. United States Department of Agriculture. USDA National Needs Graduate Fellowships. M.C. Lucy R.S. Prather, W.R. Lamberson, J.F. Taylor, D.J. Patterson, M.F. Smith, H.A. Garverick, D.H. Keisler, T.J. Safranski, E. Antoniou, J.A. Green, E.B. Rucker, P. Sutovsky, PDs. Direct costs only. $ 69,000
2005-2008. United States Department of Agriculture NRI. 2 years. Transcriptional Profiling of Nuclear Transfer-Derived Embryos to Improve Development After Nuclear Transfer. R.S. Prather, PI, P. Sutovsky & J. Green, Co-PDs $ 499,939
2005. United States Department of Agriculture. 41.0 Animal Reproduction. International Conference on Pig Reproduction. R.S. Prather, PD. Direct costs only. $ 5,000
2005-2006. Monsanto Company. Fertilization Experiments with Osteopontin. R.S. Prather, PI. $ 125,000
2005. United States Department of Agriculture. Plant and Animal Systems. International Conference on Pig Reproduction. R.S. Prather, PD. Direct costs only. $ 5,000
2004-2009. National Institutes of Health. NHLBI P01. Subcontract from the University of Iowa. Gene Therapy for Cystic Fibrosis Lung Disease. M.J. Welsh, J. Zabner, P.B. McCray, Jr., University of Iowa, PIs. R.S. Prather, PI on subcontract. $639,194 to MU. $8,424,151
2004. Immerge BioTherapeutics, Inc. Construction of Knockout Miniature Swine. R.S. Prather, PI. $ 55,013
2004-2005. National Institutes of Health. NCRR K13 RR020283. Swine in biomedical research conference. Larry Schook, Univ. IL. PI, Co-PIs Chris Counter, Duke U.; Eric Forsberg, Infigen; Merete Fredholm, Danish Vet. & Ag. Univ.; T. Mohankumar, Washington U.; Steven Niemi, Mass. General Hospital; Randall Prather, Univ. MO; and Hirosh Yasue, Japanese Nat. Inst. of Agrobiolol. Sci. $ 22,500
2003-2009; National Institutes of Health. NCRR. R24. eNOS: Metabolism & vascular biology in health & disease. M.H. Laughlin PI, E.B. Rucker Co-PI, R.S. Prather, F.W. Booth, D.K. Bowles, J.R. Turk Co-Is. $3,526,324
2003-2008; National Institutes of Health, NCRR U42. 5 years. Operation of the National Swine Research and Resource Center, Co-PIs:, L.Riley, J.K. Critser, R.S. Prather, E.B. Rucker. $7,024,385
2003-2005; National Institutes of Health, NCRR C06. 2 years. Construction of the National Swine Research and Resource Center. J.S. Coleman, PI, J.K. Critser, R.S. Prather, L.K. Riley, J. Herigon, E.B. Rucker, D. Spellman, J. Wawrzyniak, Co-PIs $2,848,226
2003-2005; University of Missouri; Match for the above C06 to construct the National Swine Research and Resource Center. J.K. Critser, L.Riley, R.S. Prather, E.B. Rucker. $5,458,530
2002-2005; United States Department of Agriculture NRI. 2 years. Bovine ESTs: Focus on female reproduction. RS Prather, PI, Co-PIs: E. Antoniou, H.A. Garverick, JA Green, MC Lucy, RM Roberts, MF Smith, RS Youngquist. $ 990,000
2002-2007; National Institutes of Health, NCRR R01. 5 years. Production of transgenic pigs by nuclear transplantation. Competitive Renewal. RS Prather, PI, E Antoniou, Co-PI. $1,034,867 2002-2003; Ximerex, Inc. 1 year. Construction of transgenic pigs for Ximerex by Nuclear Transfer. RS Prather, PI. $ 133,834
2001; United States Department of Agriculture, NRI. 6 months. 6th International Conference on Pig Reproduction. RS Prather, PI, BN Day, Co-PI. $ 5,000
2001-2002; National Institutes of Health. NCRR R01, 1 year. Production of transgenic swine by nuclear transplantation. RS Prather PI, Supplemental request to maintain genetic lines. $ 18,161
2001-2003; BioTransplant, Inc/Immerge BioTherapeutics., 2 years. Production of α-1,3-galactosyltransferase deficient pigs by nuclear transplantation. RS Prather PI (SBIR Phase II, NIH NCRR subcontract) $ 345,686
2000; BioTransplant, Inc., 6 mo. Production of α-1,3-galactosyltransferase deficient pigs by nuclear transplantation. RS Prather PI, BN Day Co-PI (SBIR Phase I, NIH NCRR subcontract) $ 45,652
1999-2004; Monsanto, 5 years. Agricultural Genomes: Animal Genome Project: Microarrays of Reproductive Significance. RS Prather & BN Day PIs, MC Lucy, MF Smith, JA Green, Co-PIs. $2,500,000
1999-2004; Monsanto, 5 years. Swine Reproduction. BN Day & RS Prather PIs, MF Smith, Co-PI. $2,500,000
1999-2002; United States Department of Agriculture NRI. 3 years. Development of Resources for Functional Genomics in the Pig. Joint project between CK Tuggle and MF Rothschild at Iowa State, MB Soares and TL Casavant at the University of Iowa, D Pomp at the University of Nebraska, WD Beavis at the National Center for Genomics Research, and RS Prather, BN Day, MC Lucy, WR Lamberson, JA Green. $124,575 to MU. $ 700,000
1999-2000; BioTransplant, Inc. 1 year. Production of transgenic mini-swine for xenotransplantation. RS Prather PI, BN Day Co-PI $ 119,755
1998-2001; National Institutes of Health. NCRR R01, 3 years. Production of transgenic swine by nuclear transplantation. RS Prather PI, BN Day Co-PI $ 595,205
1998-1999; BioTransplant, Inc., 1 year. Production of transgenic mini- swine by nuclear transplantation. RS Prather PI, BN Day Co-PI $ 113,803
1996-2000; National Institutes of Health NICHD U01. 4 years. Markers for predicting developmental competence. RS Prather, PI; BN Day & RM Roberts Co-PIs. $ 883,426
1996-1999; National Institutes of Health NCRR R01. 3 years. An improved method for making transgenic animals. RS Prather PI, BN Day Co-PI. $ 520,769
1996-1998; United States Department of Agriculture NRI, 2 years. Cdc25c and Cyclin B levels at the onset of transcription. PI $ 128,496
1996; BioTransplant, Inc. 6 months. Non-ES cell mediated gene targeting in the mini swine, via BioTransplant, Inc. subcontract. RS Prather PI, BN Day Co-PI. (NIH NCRR SBIR subcontract). $ 36,455
1995-1997; United States Department of Agriculture NRI, 2 years. Role of a G-protein coupled receptor in pig oocyte activation. PI $ 98,000
1994-1995; University of Missouri Research Council, 4 mo. Imprinting of genes in domestic pig embryos. PI. $ 2,834
1994-1995; BioTransplant, Inc., 1 year. Activation and development of mini-pig oocytes and embryos, continued. PI. $ 234,379
1993-1994; BioTransplant, Inc., 1 year. Activation and development of mini-pig oocytes and embryos. PI. $ 304,601
1992-1995; United States Department of Agriculture NRI, 3 years. Mechanisms of parthenogenic activation in pigs. PI. $ 151,108
1991-1992; National Pork Producers Council, 1 year. Artificial activation of in vitro matured porcine oocytes for cloning embryos. PI. $ 5,467
1990-1992; United States Department of Agriculture NRI, 2 years. Nuclear changes after nuclear transfer for cloning pigs. A continuation of 1988-1990, Multiplication of porcine embryos by nuclear transfer. PI. $ 150,000
1990-1991; University of Missouri Research Council, 1 year. Amino acid transport in early pig embryos. Experiments evaluate kinetics of amino acid transport in early pig embryos. PI. $ 6,222
1988-1990; National Institutes of Health Postdoctoral Fellowship, 2 1/2 years. Nuclear lamins in early embryogenesis (Awarded, but not accepted). PI. $ 60,000
1988-1990; United States Department of Agriculture CGP, 2 years. Multiplication of porcine embryos by nuclear transfer. PI. $ 200,000

Total as PI or Co-PI $56,955,547

As Major User/ Contributor/Collaborator

2007-2009; Fight SMA, The development of a swine model of Spinal Muscle Atrophy. Monique Lorson, PI. $ 172,748
2005-2008; National Science Foundation, NSF Major Instrumentation grant. University of Missouri Prime fund match. Acquisition of Instrumentation for Plant and Animal Genome Mapping and High Throughput Genotyping. H. T. Nguyen, PI. $ 860,000
2000; National Science Foundation, Minority Graduate Education Proposal. PI is Provost Dr. Brady Deaton. $2,401,784
1999: University of Missouri Research Board, Establish Genomics Core Facility. PI, Don Riddle $ 98,000
1998; National Science Foundation, Match for a new scanning electron microscope. PI is Dr. Heide Schatten. $ 273,000
1996-1999; United States Department of Agriculture, Food and Agricultural Sciences National Needs Graduate Fellowships-Animal Biotechnology Project. Funding for four highly qualified graduate students to work in the Department of Animal Sciences with the PI's or collaborators. $ 216,000
1994-1997; United States Department of Agriculture, Food and Agricultural Sciences National Needs Graduate Fellowships-Animal Biotechnology Project. Funding for three highly qualified graduate students to work in the Department of Animal Sciences with the PI's or collaborators. $ 163,000
1992-1995; United States Department of Agriculture NRI, 3 years. Transgenic expression in bovine blastocysts derived by in vitro procedures. R. M. Roberts PI, R.S. Prather Co-PI . $ 200,000
1992-1995; United States Department of Agriculture, Food and Agricultural Sciences National Needs Graduate Fellowships-Animal Biotechnology Project. Funding for three highly qualified graduate students to work in the Department of Animal Sciences with the PI's or collaborators. $ 162,000
1990-1993; United States Department of Agriculture, Food and Agricultural Sciences National Needs Graduate Fellowships-Animal Biotechnology Project. Funding for two highly qualified graduate students to work in the Department of Animal Sciences with the PI's or collaborators. $ 96,000

Total as Major User/Contributor/Collaborator $4,942,532

Awards Total $61,898,979

Gifts

Pig Improvement Company, Research on Swine Reproduction, R.S. Prather and B.N. Day, 1998. $ 5,000
Struther’s, Research on Swine Reproduction, R.S. Prather and B.N. Day, 1999 $ 8,000
Korea NLRI-RDA. Swine Reproduction, B.N. Day and R.S. Prather, 1999-2001. $111,000
BioTransplant, Inc. Micromanipulator, microscope and isolation table. R.S. Prather, 2001. $ 39,761
Circle A Ranch, 50 Angus heifers, 2002 $100,500
Immerge Biotherapeutics. 2004 $ 15,500
Immerge Biotherapeutics. 2004. Nutringer Nursery Units $ 10,000

Total Gifts $289,761

Total Dollars Awarded $62,187,835

Other

2007-2009; United States Department of Agriculture NRI, Regulation of the development o fIVF and SCNT embryos by MicroRNAs. Postdoctoral Fellowship to Dr. Jason Ross. R.S. Prather – mentor. $124,939.

Grants Pending

2009-2014. National Institutes of Health subcontract from the University of Iowa. Cystic fibrosis lung disease. R. S. Prather, PI on the subcontract. $366,128.
2009. Exemplar, Inc. Subcontract on an SBIR. Transgenic pigs for Exemplar. R.S. Prather, PI. $129,685.
2009; Juvenile Diabetes Research Foundation. Subcontract from the University of British Columbia. Tim Kieffer. Collaborator Yifan Dai at Pittsburgh. R.S. Prather, PI on Subcontract $62,461.
2009-2011; National Institutes of Health, R21. High throughput cryopreservation of swine embryos. R.S. Prather, PI. $373,755.

Publications

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8 Patents

205 Peer-Reviewed Manuscripts (28 invited)

32 Invited Review Papers/Book Chapters (not peer-reviewed)

88,042 Genome Sequence Data Base Submissions (GenBank)

232 Abstracts

Journal Cover Illustrations

• Developmental Biology 1990 Vol. 138 #2
• Journal of Animal Science 1996 Vol. 74 #9
• Animal Biotechnology 2001 Vol. 12 #2
• Biology of Reproduction 2004 Vol. 70 #6
• Reproduction 2005 Vol. 129 #4
• Reproduction, Fertility and Development 2005 Vol. 17 #8
• Molecular Reproduction and Development 2006 Vol. 73 #9
• Stem Cells and Development 2006 Vol. 15 #4
• Biology of Reproduction 2007 Vol. 77 #5
• Molecular Reproduction and Development 2008 Vol. 75 #2
• Journal of Cellular Physiology 2008 Vol. 217 #2

Paper of the Week

Journal of Biological Chemistry 2007, April 20.

Patents

1. R.S. Prather, F. Barnes, J. Robl, N.L. First & V.F. Simmon, "Multiplying Bovine Embryos"; U.S. #4,994,384 (issued February 19, ‘91).
2. J. Li, A. Rieke, B. Day & R.S. Prather, "Method of Effecting Embryo Transplant"; U.S. #5,558,636 (issued September 24, ‘96).
3. J. Li, A. Rieke, B. Day & R.S. Prather, "A System for Effecting Embryo Transplant"; U.S. #5,656,010 (issued August 12, ‘97).
4. J. Li, A. Rieke, B. Day & R.S. Prather, “A System for Introducing a Fluid into the Uterus of an Animal”; U.S. #5,916,144 (issued June 29, ‘99).
5. J. Li, A. Rieke, B. Day & R.S. Prather, “A System for Introducing a Fluid into the Uterus of an Animal”; Australia #713345 (issued March 16, ’00).
6. J. Li, A. Rieke, B. Day & R.S. Prather, “A method for Introducing a Fluid into the Uterus of an Animal”; Australia #717684 (issued July 13, ’00).
7. Z. Machaty & R.S. Prather, “Complete Activation of Mammalian Oocytes”. U.S. 6,211,429 (issued April 3, 01).
8. J. Li, A. Rieke, B. Day & R.S. Prather, “A System for Introducing a Fluid into the Uterus of an Animal”; Canada. #2,220,550 (issued April 17, ’01).
9. Prather, R.S., B.N. Day & R. Hawley. “Knockout swine and methods for making the same”, Provisional Patent filed December 20, ’01. Application # 10/499,407.
10. Prather, R.S., “Improvement of fertilization and development of in vitro produced embryos”, Provisional Patent filed June 25, ’04.
11. Welsh, M.J., C. Rogers, R.S. Prather, J. Engelhardt, Z. Yan. “Transgenic Animal Models of Disease”, Provisional patent filed March 30, ’07, Utility Patent Application filed March 5, ‘08.
12. Li, R., L. Spate, C.N. Murphy, R.S. Prather. “High throughput cryopreservation of swine embryos”, Provisional Patent filed August 29, ’08. US61/190,515.

Peer-Reviewed

1. Prather, R.S. and N.L. First. 1986. Reprogramming of murine blastocoele formation. J. Exp. Zool. 237:347-350.
2. Prather, R.S. and N.L. First. 1986. Effect of cytochalasin B and demecolcine on freeze-thaw survival of murine embryos in vitro. J. Exp. Zool. 239:37-40.
3. Prather, R.S., F.L. Barnes, M.L. Sims, J.M. Robl, W.H. Eyestone, and N.L. First.1987. Nuclear transplantation in the bovine embryo: assessment of donor nuclei and recipient oocyte. Biol. Reprod. 37:859-866.
4. Robl, J.M., R. Prather, F. Barnes, W. Eyestone, D. Northey, B. Gilligan, and N.L. First.1987. Nuclear transplantation in bovine embryos. J. Anim. Sci. 64:642-647.
5. Prather, R.S. and N.L. First. 1987. Developmental fate of highly asynchronous chimeric mouse embryos. J. Exp. Zool. 242:27-33.
6. Prather, R.S., M.F. Spire and R.R. Schalles. 1987. Evaluation of cryopreservation techniques for bovine embryos. Theriogenology 28:195-204.
7. Prather, R.S. and N.L. First. 1988. Chimerization of highly asynchronous murine blastomeres: developmental alteration? Gamete Res.19:567-576.
8. Prather, R.S. 1988. Reproductive biotechnology: an animal scientist's perspective. J. American Scientific Affiliation. 40:138-142.
9. Prather, R.S. and N.L. First. 1988. A review of early murine development and its application to domestic animals. J. Anim. Sci. 66:2626-2635.
10. Dean, W.L., A.C. Seufert, G.A. Schultz, R.S. Prather, C. Simerly, G. Schatten, D.R. Pilch and W.F. Marzluff. 1989. The small nuclear RNAs for pre-mRNA splicing are coordinately regulated during oocyte maturation and early embryogenesis in the mouse. Development 106:325-334.
11. Prather, R.S., M.M. Sims, G.G. Maul, N.L. First and G. Schatten. 1989. Nuclear lamin antigens are developmentally regulated during porcine and bovine embryogenesis. Biol. Reprod. 41:123-132.
12. Prather, R.S., M.M. Sims and N.L. First. 1989. Nuclear transplantation in early pig embryos. Biol. Reprod. 41:414-418.
13. Prather, R., C. Simerly, G. Schatten, D.R. Pilch, S.M. Lobo, W.F. Marzluff, W.L. Dean and G.A. Schultz. 1990. U3 snRNPs and nucleolar development during oocyte maturation, fertilization and early embryogenesis in the mouse: U3 snRNA and snRNPs are not regulated coordinate with other snRNAs and snRNPs. Dev. Biol. 138:247-255.
14. Prather, R.S., M.M. Sims and N.L. First. 1990. Nuclear transplantation in the pig embryo: nuclear swelling. J. Exp. Zool. 255:355-358.
15. Jarrell, V.L., B.N. Day and R.S. Prather. 1991. The transition from maternal control of development to zygotic control of development occurs during the 4-cell stage in the domestic pig, Sus scrofa: quantitative and qualitative aspects of protein synthesis. Biol. Reprod. 44:62-68.
16. Hagen, D.R., R.S. Prather, M.M. Sims and N.L. First. 1991. Development of one-cell porcine embryos to the blastocyst stage in simple media. J. Anim. Sci. 69:1147-1150.
17. Prather, R.S., J. Kubiak, G.G. Maul, N.L. First and G. Schatten. 1991. The association of nuclear lamins A and C is regulated by the developmental stage of the mouse oocyte or embryonic cytoplasm. J. Exp. Zool. 257:110-114.
18. Kubiak, J.Z., R.S. Prather, G.G. Maul and G. Schatten. 1991. Cytoplasmic modification of the nuclear lamina during pronuclear-like transformation of mouse blastomere nuclei. Mech. Devel. 35:103 111.
19. Hagen, D.R., R.S. Prather and N.L. First. 1991. Response of porcine oocytes to electrical and chemical activation during maturation in vitro. Mol. Reprod. & Dev. 28:70-73.
20. Prather, R.S., P.A. Eichen, D.K. Nicks and M.S. Peters. 1991. Artificial activation of porcine oocytes matured in vitro. Mol. Reprod. & Dev. 28:405-409.
21. Prather, R.S., M.M Sims and N.L. First. 1991. Culture of porcine embryos from one- and two-cell to blastocyst in sheep oviducts. Theriogenology 35:1147-1151.
22. Schoenbeck, R.A., M.S. Peters, L.F. Rickords, T.T. Stumpf and R.S. Prather. 1992. Characterization of DNA synthesis and the transition from maternal to embryonic control in the 4-cell porcine embryo. Biol. Reprod. 47:1118-1125.
23. Boice, M.L., P.A. Mavrogianis, C.N. Murphy, R.S. Prather and B. N. Day. 1992. Immunocytochemical analysis of the association of bovine oviduct-specific secretary glycoproteins with early embryos. J. Exp. Zool. 263:225-229.
24. Prather, R.S. and G. Schatten. 1992. Construction of the nuclear matrix at the transition from maternal to zygotic control of development in the mouse: an immunocytochemical study. Mol. Reprod. & Dev. 32:203-208.
25. Prather, R.S. and L.F. Rickords. 1992. Developmental regulation of a snRNP core protein epitope during pig embryogenesis and after nuclear transfer for cloning. Mol. Reprod. & Dev. 33:119-123.
26. Prather, R.S., M.S. Peters and L.J. Van Winkle. 1993. Alanine and leucine transport in unfertilized pig oocytes and early blastocysts. Mol. Reprod. & Dev. 34:250-254.
27. Prather, R.S., M.S. Peters and L.J. Van Winkle. 1993. Aspartic and glutamic acid transport in unfertilized pig oocytes and blastocysts. Mol. Reprod. & Dev. 36:49-52.
28. Prather, R.S. and N.L. First. 1993. Cell-to-cell coupling in early bovine embryos. Theriogenology 39:561-567.
29. Schoenbeck, R.A., M.S. Peters, L.F. Rickords, T.T. Stumpf, S.L. Terlouw and R.S. Prather. 1993. Diacylglycerol enhanced electrical activation of porcine oocytes matured in vitro. Theriogenology 40:257-266.
30. Macháty, Z., M.A. Mayes and R.S. Prather. 1995. Activation of the G protein in porcine oocytes. Biol. Reprod. 52:753-758.
31. Mayes, M.A., P.A. Stogsdill and R.S. Prather. 1995. Parthenogenetic activation of pig oocytes by protein kinase inhibition. Biol. Reprod. 53:270-275.
32. Tilton, S.L., R.O. Bates, B.N. Day and R.S. Prather. 1995. Evaluation of response to hormonal therapy in prepubertal gilts of different genetic lines. J. Anim. Sci. 73:3062-3068.
33. Nussbaum, D.J. and R.S. Prather. 1995. Effects of protein synthesis inhibitors on porcine oocyte activation. Mol. Reprod. & Dev. 41:70-75.
34. Parry, T.W. and R.S. Prather. 1995. Protein reprogramming after nuclear transfer? Reproduction, Nutrition Development 35:313-318.
35. Prather, R.S., M.L. Boice, J. Gibson, K. Hoffman and T.W. Parry. 1995. In vitro development of embryos from Sinclair miniature pigs. Theriogenology 43:1001-1007.
36. Macháty, Z., M.A. Mayes, H. Funahashi, B.N. Day and R.S. Prather. 1996. Effects of injecting in vitro matured porcine oocytes with CaCl2. Biol. Reprod. 54:316-322.
37. Li, J. A. Rieke, B.N. Day, and R.S. Prather. 1996. A tool and technique for non-surgical embryo transfer in pigs. J. Anim. Sci. 74:2263-2268.
38. Prather, R.S., J. Gibson, and T.W. Parry. 1996. Artificial activation of oocytes from Sinclair miniature pigs. J. Applied Anim. Res. 10:125-134.
39. Kim, N.-H., H. Funahashi, L.R. Abeydeera, S.-J. Moon, R.S. Prather and B.N. Day. 1996. Effects of oviductal fluid on sperm penetration and cortical granule exocytosis during in vitro fertilization of porcine oocytes. J. Reprod. Fert. 107:79-86.
40. Kim, N.-H., H. Funahashi, R.S. Prather, G. Schatten, B.N. Day. 1996. Microtubule and microfilament dynamics in porcine oocytes during meiotic maturation. Mol. Reprod. & Dev. 43:248-255.
41. Kim, N.-H., S.-J. Moon, R.S. Prather and B.N. Day. 1996. Cytoskeletal alteration in aged porcine oocytes and parthenogenesis. Mol. Reprod. & Dev. 43:513-518.
42. Prather, R.S., T.T. Stumpf, R.A. Schoenbeck, K.E. Hoffman, and J. Li. 1996. Characterization of DNA synthesis during the 2-cell stage and the production of tetraploid chimeric pig embryos. Mol. Reprod. & Dev. 45:38-42.
43. Funahashi, H., Z. Macháty, R.S. Prather, and B.N. Day. 1996. g-Glutamyl transpeptidase of spermatozoa may reduce oocyte glutathione content at fertilization in pigs. Mol. Reprod. & Dev. 45:485-490.
44. Jolliff, W.J., and R.S. Prather. 1997. Parthenogenetic development of in vitro matured, in vivo cultured porcine oocytes beyond blastocyst. Biol. Reprod. 56:544-548.
45. Macháty, Z., H. Funahashi, B.N. Day and R.S. Prather. 1997. Developmental changes in the intracellular calcium release mechanisms in porcine oocytes. Biol. Reprod. 56:921-930.
46. Macháty, Z., M.A. Mayes, L. Kovacs, P. Balatti, J.-H. Kim, and R.S. Prather. 1997. Activation of porcine oocytes via an exogenously introduced rat muscarinic M1 receptor. Biol. Reprod. 57:85-91.
47. Macháty, Z., W.-H. Wang, B.N. Day, and R.S. Prather. 1997. Complete activation of porcine oocytes induced by the sulphydryl reagent, thimerosal. Biol. Reprod. 57:1123-1127.
48. Prather, R.S., Mayes, M.A. and C.N. Murphy. 1997. Parthenogenetic activation of pig oocytes by extended exposure to low levels of broad-spectrum protein kinase inhibitors. Reprod. Fert. Dev. 9:539-544.
49. Prather, R.S., J. Gibson, K. Hoffman and T.W. Parry. 1997. Reproductive measurements in Sinclair and NIH miniature pigs: a retrospective analysis. Theriogenology 47:233-440.
50. Wang, W.-H., Z. Macháty, L.R. Abeydeera, R.S. Prather and B.N. Day. 1998. Parthenogenetic activation of pig oocytes with calcium ionophore and the establishment of the zona block to sperm penetration after activation. Biol. Reprod. 58:1357-1366.
51. Abeydeera, L.R., W.-H. Wang, R.S. Prather, and B.N. Day. 1998. Maturation in vitro of pig oocytes in protein-free culture media: Fertilization and subsequent embryo development in vitro. Biol. Reprod. 58:1316-1320.
52. Macháty, Z., B.N. Day, R.S. Prather. 1998. Development of early pig embryos in vitro and in vivo. Biol. Reprod. 59:451-455.
53. Kim, J.-H., Z. Macháty, R.A. Cabot, Y.-M. Han, H.-J. Do, and R.S. Prather. 1998 Development of pig oocytes activated by stimulation of an exogenous G-protein coupled receptor. Biol. Reprod. 59:655-660.
54. Wang, W.-H., L.R. Abeydeera, R.S. Prather and B.N. Day. 1998. Morphological comparison of ovulated and in vitro matured porcine oocytes, with particular reference to polyspermy after in vitro fertilization. Mol. Reprod. & Dev. 49:308-316.
55. Wang, W.-H., L.A. Abeydeera, R.S. Prather and B.N. Day. 1998. Functional analysis of activation of porcine oocytes by spermatozoa, calcium ionophore and electrical pulse. Mol. Reprod. Dev. 51:346-353.
56. Abeydeera, L.A., W.-H. Wang, T.C. Cantley, A. Rieke, R.S. Prather, and B.N. Day. 1998. Presence of epidermal growth factor during in vitro maturation of pig oocytes and embryo culture can modulate blastocyst development after in vitro fertilization. Mol. Reprod. & Dev. 51:395-401.
57. Abeydeera, L.A., W.-H. Wang, T.C. Cantley, R.S. Prather, and B.N. Day. 1998. Presence of b-mercaptoethanol can increase the glutathione content of pig oocytes matured in vitro and the rate of blastocyst development after in vitro fertilization. Theriogenology 50:747-756.
58. Tao, T., Z. Macháty, A.C. Boquest, B.N. Day and R.S. Prather. 1999. Development of pig embryos reconstructed by microinjection of cultured fetal fibroblast cells into in vitro matured oocytes. Anim. Reprod. Sci. 56:133-141.
59. Boquest, A.C., B.N Day, and R.S. Prather. 1999. Flow cytometric cell cycle analysis of cultured porcine fetal fibroblast cells used for nuclear transfer. Biol. Reprod. 60:1013-1019.
60. Wang, W.-H., L.R. Abeydeera, Y.-M. Han, R.S. Prather, and B.N. Day. 1999. Morphologic evaluation and actin distribution in porcine embryos produced in vitro and in vivo. Biol. Reprod. 60:1020-1028.
61. Han, Y.-M, L.R. Abeydeera, J.-H. Kim, B.N. Day, H.-B. Moon, and R.S. Prather. 1999. Growth retardation of inner cell mass cells in polyspermic pig embryos produced in vitro. Biol. Reprod. 60:1110-1113.
62. Macháty, Z., W.-H. Wang, B.N. Day and R.S. Prather. 1999. Calcium release and subsequent development induced by modification of sulfhydryl groups in porcine oocytes. Biol. Reprod. 60:1384-1391.
63. Green, K.M., J.H. Kim, W.-H. Wang, B.N. Day, and R.S. Prather. 1999. The effect of myosin light chain kinase, protein kinase A and protein kinase C inhibition on porcine oocyte activation. Biol. Reprod. 61:111-119.
64. Kim, J.-H., H.-J. Do, W.-H. Wang, Z. Macháty, Y.-M. Han, B.N. Day and R.S. Prather.1999. A protein tyrosine phosphatase inhibitor, sodium orthovanadate, causes parthenogenetic activation of pig oocytes via an increase in protein tyrosine kinase activity. Biol. Reprod. 61:900-905.
65. Han, Y.-M, L.R. Abeydeera, W.-H. Wang, A.L. Petersen, J.-H. Kim, C. Murphy, B.N. Day, and R.S. Prather. 1999. Pronuclear location before the first cell division determines ploidy of polyspermic pig embryos. Biol. Reprod. 61:1340-1346.
66. Anderson, J.E., R.L. Matteri, L.R. Abeydeera, B.N. Day and R.S. Prather. 1999. Cyclin B1 transcript quantitation over the maternal to zygotic transition in both in vitro and in vitro derived 4-cell porcine embryos. Biol. Reprod. 61:1460-1467.
67. Prather. R.S., A.C. Boquest, and B.N. Day. 1999. Cell cycle analysis of cultured porcine mammary cells. Cloning 1:17-24.
68. Tao, T., A.C. Boquest, Z. Macháty, A.L. Petersen, B.N. Day and R.S. Prather. 1999. Development of pig embryos by nuclear transfer of cultured fetal fibroblast cells. Cloning 1:55-62.
69. Macháty, Z., L.F. Rickords and R.S. Prather. 1999. Parthenogenetic activation of porcine oocytes following nuclear transfer. Cloning 1:101-109.
70. Holm, P, G. Vajta, Z. Macháty, M. Schmidt, R.S. Prather, T. Greve. 1999. Open pulled straw (OPS) vitrification of porcine blastocysts: simple procedure yielding excellent in vitro survival, but so far no piglets following transfer. CryoLetters 20:307-310.
71. Wang, W.-H., Z. Macháty, N. Ruddock, L.R. Abeydeera, R.S. Prather, and B.N. Day. 1999. Activation of porcine oocytes with calcium ionophore: effects of extracellular calcium. Mol. Reprod. Dev. 53:99-107.
72. Vajta, G, C. Murphy, Z. Macháty, R.S. Prather, T. Greve, and H. Callesen. 1999. In-straw dilution of in vitro produced bovine blastocysts after vitrification with the open pulled straw (OPS) method. Vet. Record. 144:180-181.
73. Wang, W.-H., Z. Macháty, L.R. Abeydeera, R.S. Prather, and B.N. Day. 1999. Time course of cortical and zona reactions of pig oocytes upon intracellular calcium increase induced by thimerosal. Zygote 7:79-86.
74. Abeydeera, L.R., W.-H. Wang, T.C. Cantley, R.S. Prather, and B.N. Day. 1999. Glutathione content and embryo development after in vitro fertilization of pig oocytes matured in the presence of a thiol compound and various concentrations of cysteine. Zygote 7:203-210.
75. Wang, W.H., L.R. Abeydeera, R.S. Prather and B.N. Day. 2000. Polymerization of non-filamentous actin into microfilaments is an important process for porcine oocyte maturation and early embryo development. Biol. Reprod. 62:1177-1183.
76. Ruddock, N.T., Z. Macháty, M. Milanick and R.S. Prather. 2000. Mechanism of intracellular pH increase during parthenogenetic activation of in vitro matured porcine oocytes. Biol. Reprod. 63:488-492.
77. Kühholzer, B., T. Tao, Z. Macháty, B. Hawley, J. Greenstein, B.N. Day and R.S. Prather. 2000. Production of transgenic porcine blastocysts by nuclear transfer. Mol. Reprod. Dev. 56:145-148.
78. Macháty, Z., A. Bonk, B. Kühholzer and R.S. Prather. 2000. Porcine oocyte activation induced by a cytosolic sperm factor. Mol. Reprod. Dev. 57:290-295.
79. Ruddock, N.T., Z. Macháty and R.S. Prather. 2000. Intracellular pH increases accompany parthenogenetic activation of porcine, bovine and murine oocytes. Reprod. Fertil. & Develop. 12:201-207.
80. Abeydeera, L.R. W.H. Wang, T.C. Cantley, A. Rieke, R.S. Prather and B.N. Day. 2000. Development and viability of pig oocytes matured in a protein-free medium containing epidermal growth factor. Theriogenology 54(5):787-797
81. Tao, T., Z. Macháty, L.R. Abeydeera, B.N. Day, and R.S. Prather. 2000. Optimization of porcine oocyte activation following nuclear transfer. Zygote 8: 69-77.
82. Wang, W.H., L.R. Abeydeera, R.S. Prather, B.N. Day. 2000. Actin filament distribution in blocked and developing pig embryos. Zygote 8: 353-358.
83. Park, K.-W., H -T. Cheong, L. Lai, G.-S. Im, B. Kűhholzer, A. Bonk, M. Samuel, A. Rieke, B.N. Day, C.N. Murphy, D.B. Carter, R.S. Prather. 2001. Production of nuclear transfer-derived swine that express the enhanced green fluorescent protein. Anim. Biotech. 12:(2) 173-181.
84. Cabot, R., B. Kühholzer, A.W. Chan, L. Lai, K.W. Park, K.Y. Chong, G. Schatten, C.N. Murphy, L.R. Abeydeera, B.N. Day, R.S. Prather. 2001. Production of transgenic piglets derived from in vitro matured oocytes infected with a replication deficient retroviral vector. Anim. Biotech. 12:(2) 205-214.
85. Shi, X.W., C.J. Fitzsimmons, C. Genêt, R.S. Prather, K. Whitworth, J.A. Green, C. K. Tuggle. 2001. High-resolution radiation hybrid mapping of human chromosome 17 genes to the pig genome extends gene order conservation with pig chromosome 12 and limited synteny with pig chromosome 2. Animal Genetics 32:205-209.
86. Kühholzer, B. and R.S. Prather. 2001. Reversible synchronization of porcine fetal fibroblast cells with the topoisomerase-inhibitor hoechst 33342. Anim. Repro. Sci. 66:109-116.
87. Park, K.-W., B. Kuhholzer, L. Lai, Z. Macháty, Q.-Y. Sun, B.N. Day, and R.S. Prather. 2001. Development and expression of the green fluorescent protein in porcine embryos derived from nuclear transfer of transgenic granulosa-derived cells. Anim. Repro. Sci. 68:111-120.
88. Sun, Q.-Y., L. Lai, K.-W. Park, B. Kühholzer, R.S. Prather and H. Schatten. 2001 Dynamic events are differently mediated by microfilaments and microtubules during porcine maturation and fertilization. Biol. Reprod. 64:879-889.
89. Kühholzer, B., R.J. Hawley, L. Lai, D. Kolber-Simonds, R.S. Prather. 2001. Nuclear transfer using different sub-clones of porcine fetal fibroblast cells result in different in vitro development. Biol. Reprod. 64:1695-1698.
90. Lai, L., T. Tao, Z. Macháty, B. Kűhholzer, K.W. Park, B.N. Day, and R.S. Prather. 2001. Feasibility of producing porcine nuclear transfer embryos by using G2/M stage fibroblasts as donors. Biol. Reprod. 65:1558-1564.
91. Park, K.-W., L. Lai, H.-T. Cheong, G.-S. Im, Q.-Y. Sun, G. Wu, B.N. Day, and R.S. Prather. 2001. Developmental potential of nuclear transfer embryos derived from transgenic fetal fibroblasts infected with the gene for the green fluorescent protein: comparison of different fusion/activation conditions. Biol. Reprod. 65:1681-1685.
92. Jiang, H., N.J. Bivens, J.E. Ries, K.M. Whitworth, J.A. Green, L.J. Forrester, G.K. Springer, B.A. Didion, N. Mathialagan, R.S. Prather and M.C. Lucy. 2001. Constructing cDNA libraries with fewer clones that contain long poly(dA) tails. BioTechniques 31:38-42.
93. Macháty, Z., J. Thompson, B.N. Day and R.S. Prather. 2001. Inhibitors of mitochondrial ATP production at the time of compaction improve development of in vitro produced porcine embryos. Mol. Reprod. Dev. 58:39-43.
94. Sun, Q.-Y., L. Lai, A. Bonk, R.S. Prather and H. Schatten. 2001. Cytoplasmic changes in relation to nuclear maturation and early embryo development potential of porcine oocytes: effects of gonadotropins, cumulus cells, follicular sizes and protein synthesis inhibition. Mol. Reprod. Dev. 59:192-198.
95. Ruddock, N.T., Z. Macháty and R.S. Prather. 2001. Porcine activation: importance of intracellular increases in calcium and pH. Mol. Reprod. Dev. 59:227-234.
96. Anderson, J.E., R.L. Matteri, L.R. Abeydeera, B.N. Day and R.S. Prather. 2001. Degradation of maternal cdc25c during the maternal to zygotic transition is dependent upon embryonic transcription. Mol. Reprod. Dev. 60:181-188.
97. Wei, Z., K.-W. Park, B.N. Day and R.S. Prather. 2001. The effect of epidermal growth factor on preimplantation development and its receptor expression in porcine embryos. Mol. Reprod. Dev. 60:457-462.
98. Sun, Q.-Y., L. Lai, G. Wu, K.-W. Park, B. Day, R.S. Prather, and H. Schatten. 2001. Microtubule assembly after treatment of pig oocytes with taxol: correlation with chromosomes, gamma-tubulin and MAP kinase. Mol. Reprod. Dev. 60:481-490.
99. Sun Q.-Y., G.M. Wu, L. Lai, K.-W. Park, B. Day, R.S. Prather and H. Schatten. 2001. Translocation of active mitochondria during porcine oocyte maturation, fertilization and early embryo development in vitro. Reproduction 122:155-163.
100. Sun, Q.-Y., L. Lai, R.S. Prather and H. Schatten. 2001. Antioxidants stimulate meiosis resumption, but inhibit MAP kinase phosphorylation and further cell cycle progression in porcine oocytes. Reprod. Fertil. & Develop. 12:383-389.
101. Do, H.J., J.H. Kim, L.R. Abeydeera, Y.M. Han, J. Green, R.M. Roberts, R.L. Matteri, B.N. Day, and R.S. Prather. 2001. Expression of pregnancy-associated glycoprotein 1 and 2 genes in in vitro-, in vivo- and parthenogenetically-derived preimplantation pig embryos. Zygote 9:245-250.
102. Abeydeera, L.R., W.-H. Wang, R.S. Prather, B.N. Day. 2001. Effect of incubation temperature on in vitro maturation of porcine oocytes: nuclear maturation, fertilization and developmental competence. Zygote 9:331-337.
103. Lai, L., Q. Sun, G. Wu, C.N. Murphy, B. Kühholzer, K.-W. Park, A.J. Bonk, B.N. Day, and R.S. Prather. 2001. Development of porcine embryos and offspring after ICSI with liposome transfected or non-transfected sperm into in vitro matured oocytes. Zygote 9:339-346.
104. Sun, Q.-Y, G.-M. Wu, L. Lai, A. Bonk, R. Cabot, K.-W. Park, B.N. Day, R.S. Prather, H. Schatten. 2002. Regulation of mitogen-activated protein kinase phosphorylation, microtubule organization, chromatin behavior, and cell cycle progression by protein phosphatases during pig oocyte maturation and fertilization in vitro. Biol. Reprod. 66:580-588.
105. Machaty, Z., J.J. Ramsoondar, A.J. Bonk, K.R. Bondioli, and R.S. Prather. 2002. Capacitative calcium entry mechanism in porcine oocytes. Biol. Reprod. 66:667-674.
106. Park, K.-W., L. Lai, H.-T. Cheong, R. Cabot, Q.-Y. Sun, G. Wu, E.B. Rucker, D. Durtschi, A. Bonk, M. Samuel, A. Rieke, B.N. Day, C.N. Murphy, D.B. Carter, R.S. Prather. 2002. Mosaic Gene Expression in Nuclear Transfer-Derived embryos and the Production of Cloned Transgenic Pigs from Ear-derived Fibroblasts. Biol. Reprod. 66:1001-1005.
107. Wu, G.-M., Q.-Y. Sun, J. Mao, L. Lai, T.C. McCauley, K.-W. Park, R.S. Prather, B.A. Didion, and B.N. Day. 2002. High developmental competence of pig oocytes after meiotic inhibition with a specific MPF kinase inhibitor, butyrolactone I. Biol. Reprod. 67:170-177.
108. Cabot, R.A., M. Hannink, R.S. Prather. 2002. CRM1 –mediated nuclear export is present during mammalian embryogenesis, but is not required for early cleavage. Biol. Reprod. 67:814-819.
109. Z. Macháty, J.J. Ramsoondar, A.J. Bonk, R.S. Prather, K.R. Bondioli. 2002. The Na+/Ca2+ exchanger in porcine oocytes. Biol. Reprod. 67:1133-1139.
110. Mao, J., G. Wu, M.F. Smith, R.S. Prather, B.A. Didion, B.N. Day. 2002. Effects of culture medium, serum type and various concentrations of FSH on porcine preantral follicular development and antrum formation in vitro. Biol. Reprod. 67:1197-1203.
111. Carter, D.B., L. Lai, K.-W. Park, M. Samuel, J.C. Lattimer, K.R. Jordan, D.M Estes, C. Besch-Williford, R.S. Prather. 2002. Phenotyping of transgenic cloned piglets. Cloning & Stem Cells 4:131-145.
112. Cheong, H.-T., K.-W. Park, G.-S. Im, L. Lai, Q.-Y. Sun, B.N. Day, R.S. Prather. 2002. Effect of elevated calcium concentration in fusion/activation medium on the fusion and development of porcine fetal fibroblast cell nuclear transfer embryos. Mol. Reprod. & Dev. 61:488-492.
113. Lai, L., K.-W. Park, H.-T. Cheong, B. Kühholzer, M. Samuel, A. Bonk, G.-S. Im, A. Rieke, B.N. Day, C.N. Murphy, D.B. Carter, R.S. Prather. 2002. A transgenic pig expressing the green fluorescent protein produced by nuclear transfer using colchicine-treated fibroblasts as donor cells. Mol. Reprod. Dev. 62:300-306.
114. Lai, L., D. Kolber-Simonds, K.-W. Park, H.-T. Cheong, J.L. Greenstein, G.-S. Im, M. Samuel, A. Bonk, A. Rieke, B.N. Day, C.N. Murphy, D.B Carter, R.J. Hawley, R.S. Prather. 2002. Production of α-1, 3-galactosyltransferase-knockout inbred miniature swine by nuclear transfer cloning. Science 295:1089-1092. Published online January 3, 2002; 10.1126/science.1068228 (Science Express Reports).
115. Bonk, A.J., J.E. Anderson, L.R. Abeydeera, B.N. Day, and R.S. Prather. 2002. Cyclin B1 levels in the porcine 4-cell stage embryo. Zygote 10:79-84.
116. Hao, Y.H., L. Lai, J. Mao, G.S. Im, A. Bonk and R.S. Prather. 2003. Apoptosis and in vitro development of preimplantation porcine embryos derived in vitro or by nuclear transfer. Biol. Reprod. 69:501-507.
117. Tuggle, C.K., J.A. Green, C. Fitzsimmons, R. Woods, R.S. Prather, S. Malchenko, M.B. Soares, D. Tack, N. Robinson, B. O’Leary, T. Scheets,, T. Casavant, D. Pomp, J.B. Edeal, Y. Zhang, M.F. Rothschild, K. Garwood, W. Beavis. 2003. EST-basted gene discovery in pig: virtual expression patterns and comparative mapping to human. Mammalian Genome 14: 565-579.
118. Cabot, R.A., R.S. Prather. 2003. Cleavage stage porcine embryos have differing developmental requirements for karyopherins α2 and α3. Mol. Reprod. Dev. 64:292-301.
119. Martinez, E.A., J.N. Caamano, M.A. Gil, A. Rieke, T.C. McCauley, T.C. Cantley, J.M. Vazquez, J. Roca, J.L. Vazquez, C.N. Murphy, B.A. Didion, R.S. Prather, B.N. Day. 2003. Successful non-surgical deep uterine embryo transfer in pigs. Theriogenology 61:137-146.
120. Jiang, L., D.B. Carter, J. Xu, X. Yang, R.S. Prather, X.C. Tian. 2004. Telomere lengths in cloned transgenic pigs. Biol. Reprod. 70:1589-1593.
121. Hao, Y., L. Lai, J. Mao, G.S. Im, A. Bonk and R.S. Prather. 2004. Apoptosis in parthenogenetic preimplantation porcine embryos. Biol. Reprod. 70:1644-1649.
122. Thompson, W.E., E. Asselin, A. Branch, J.K. Stiles, P. Sutovsky, L. Lai, G.S. Im, R.S. Prather, S.C. Isom, E.B. Rucker, B.K. Tsang. 2004. Regulated expression and potential role of prohibitin gene product during follicular development and atresia in the mammalian ovary. Biol. Reprod. 71: 282-290.
123. Whitworth, K., G.K. Springer, L.J. Forrester, W.G. Spollen, J. Ries, W.R. Lamberson, N. Bivens, C.N. Murphy, N. Mathialagan, J.A. Green, and R.S. Prather. 2004. Developmental expression of 2,489 genes during pig embryogenesis: an EST project. Biol. Reprod. 71:1230-1243.
124. Jiang, H. , N. Bivens, J. Ries, R. Woods, K.M. Whitworth, J.A. Green, L.J. Forrester, G.K. Springer, N. Mathialagan, C. Agca, R.S. Prather, M.C. Lucy. 2004. Large-scale generation and analysis of expression sequence tags from porcine ovary. Biol. Reprod. 71:1991-2002.
125. Mao, J., M.F. Smith, E.B. Rucker, G. Wu, T.C. McCauley, T.C. Cantley, R.S. Prather, B.A. Didion, and B.N. Day. 2004. Effect of epidermal growth factor and insulin-like growth factor I on porcine preantral follicular growth, antrum formation, and stimulation of granulose cell proliferation, and suppression of apoptosis, in vitro. J. Anim. Sci. 82:1967-1975.
126. Im, G.-S., L. Lai, Z. Liu, Y. Hao, D. Wax, A. Bonk, R.S. Prather. 2004. In vitro development of preimplantation porcine nuclear transfer embryos cultured in different media and gas atmospheres. Theriogenology. 61:1125-1135.
127. Wu, G.-M., L. Lai, J. Mao, T.C. McCauley, J.N. Caamano, T. Cantley, A. Rieke, C. Murphy, R.S. Prather, B.A. Didion and B.N. Day. 2004. Birth of piglets by in vitro fertilization of zona-free porcine oocytes. Theriogenology 62:1544-1556.
128. Sutovsky, P. and R.S. Prather. 2004. Nuclear remodeling after SCNT: A contractor’s nightmare. Trends in Biotechnology 22:205-208.
129. Kolber-Simonds, D.*, L. Lai*, S.R. Watt*, M. Denaro*, S. Arn, M.L. Augenstein, J. Betthauser, D.B. Carter, J.L. Greenstein, Y. Hao, G.-S. Im, Z. Liu, G.D. Mell, C.N. Murphy, K.-W. Park, A. Rieke, D. Ryan, D.H. Sachs, E.J. Forsberg, R.S. Prather, and R.J. Hawley. 2004. Alpha-1,3-galactosyltransferase null pigs via nuclear transfer with fibroblasts bearing loss of heterozygosity mutations. Proc. Natl. Acad. Sci. U.S.A. 101:7335-7340.
130. Whitworth, K.W., C. Agca, J-G. Kim, R.V. Patel, G.K. Springer, N. Bivens, L.J. Forrester, N. Mathialagan, J.A. Green, R.S. Prather. 2005. Transcriptional profiling of pig embryogenesis by using a 15K member unigene set specific for the pig. Biol. Reprod. 72:1437-1451.
131. Carroll, J.A., D.B. Carter, S.W. Korte, R.S. Prather. 2005. Evaluation of the innate immune response in cloned pigs following a lipopolysaccharide challenge. Dom Anim Endo 29:564-572.
132. Im, G.-S., B.-S. Yang, L. Lai, Z. Liu, Y. Hao, R.S. Prather. 2005. Fragmentation and development of preimplantation porcine embryos derived by parthenogenetic activation and nuclear transfer. Mol. Reprod. Dev. 71:159-165.
133. Sutovsky, P., G. Manandhar, J. Laurincik, J. Letko, J.N. Caamano, B.N. Day, L. Lai, R.S. Prather, K. Sharpe-Timms, R. Zimmer, and M. Sutovsky. 2005. Expression and proteasomal degradation of the Major Vault Protein (MVP) in the mammalian oocyte and zygote. Reproduction 129: 269-282.
134. Vajta, G., P. Maddox-Hyttel, C.T. Skou, T.R. Tecirlioglu, T.T. Peura, L. Lai, C.N. Murphy, R.S. Prather, P.M. Kragh, H. Callesen. 2005. Highly efficient and reliable chemically assisted enucleation method for handmade cloning (HMC) in cattle. Reproduction, Fertility & Development 17:791-797.
135. Mao, J., G.-M. Wu, R.S. Prather, M.F. Smith, T. Cantley, A. Rieke, B.A. Didion, B.N. Day. 2005. Effect of methyl-b-cyclodextrin treatment of pig spermatozoa on in vitro fertilization and embryo development in the absence or present of caffeine. Theriogenology 64:1913-1927.
136. Li, R., L. Lai, D. Wax, Y. Hao, C. Murphy, A. Rieke, M. Samuel, M. Linville, S. Korte, R.W. Evans, J.R. Turk, J.X. Kang, W.T. Witt, Y. Dai, R.S. Prather. 2006. Cloned transgenic swine via in vitro production and cryopreservation. Biology of Reproduction 75:226-230.
137. Hao, Y., N. Mathialagan, E. Walters, J. Mao, L. Lai, D. Becker, W. Li, J. Critser, R.S. Prather. 2006. Osteopontin reduces polyspermy during IVF of porcine oocytes. Biology of Reproduction 75:726-733.
138. Katayama, M, Z. Zhong, L. Lai, P. Sutovsky, R.S. Prather, H. Schatten. 2006. Mitochondria distribution and microtubule organization in fertilized and cloned porcine embryos: implications for developmental potential. Developmental Biology 299:206-220.
139. Liu, Z.H., H. Schatten ,Y. H. Hao, L.X. Lai, D. Wax, M. Samuel, Z.S. Zhong, Q.Y. Sun, R.S. Prather. 2006. The nuclear mitotic apparatus (NuMA) protein is contributed by the donor cell nucleus in cloned porcine embryos. Frontiers in Biosciences 11:1945-1957.
140. Hao, Y., L. Lai, G.-S. Im, Z. Liu, D. Wax, M. Samuel, C.N. Murphy, P. Sutovsky, R.S. Prather. 2006. Developmental competence of porcine parthenogenetic embryos relative to embryonic chromosomal abnormalities. Mol. Reprod. Dev. 73:77-82.
141. Yong, H.Y., Y. Hao, L. Lai, R. Li, C.N. Murphy, A. Rieke, D. Wax, M. Samuel, R.S. Prather. 2006. Production of a transgenic piglet by a new sperm injection technique in which no chemical or physical treatments are used for oocytes or sperm. Mol. Reprod. & Dev. 73:595-599.
142. Im, G.-S., J.-S. Seo, I.-S. Hwang, D.-H. Kim, S.-W. Kim, B.-C. Yang, B.-S. Yang, L. Lai, R.S. Prather. 2006. Development and apoptosis of pre-implantation porcine nuclear transfer embryos activated with different combination of chemicals. Mol. Reprod. Dev. 73:1094-1101.
143. Bjerregaard, B., H.G. Pedersen, A.S. Jakobsen, L.F. Rickords, L. Lai, H.T. Cheong, M. Samuel, R.S. Prather, F. Strejcek, Z. Rasmussen, J. Laurincik, K. Schellander, H. Niemann, P. Maddox-Hyttel, P.D. Thomsen. 2006. Activation of ribosomal RNA genes among cells of in vitro produced and cloned porcine embryos. Mol. Reprod. & Dev. 74:35-41.
144. Lai, L., J.X. Kang, R. Li, J. Wang, W. Witt, H.Y. Yong, Y. Hao, D.M. Wax, C.N. Murphy, A. Rieke, M. Samuel, M. Linville, S. Korte, R. Evans, T. Starzl, R.S. Prather, Y. Dai. 2006. Fat-1 transgenic pigs produce a high level of omega-3 fatty acids. Nature Biotechnology 24:435-437.
145. Agca, C., J.E. Ries, S.J. Kolath, J.-H. Kim, L.J. Forrester, E. Antoniou, K.M. Whitworth, N. Mathialagan, G.K. Springer, R.S. Prather, M.C. Lucy. 2006. Luteinization of porcine preovulatory follicles leads to systematic changes in follicular gene expression. Reproduction 132:133-145.
146. Manandhar, G., D. Feng, Y.-J. Yi, L. Lai, T. Ezashi, J. Letko, J. Laurincik, M. Sutovsky, R.S. Prather, H. Schatten, P. Sutovsky. 2006. Centrosomal protein Centrin is not detectable during early pre-implantation development but reappears during the late blastocyst stage in porcine embryos. Reproduction 312:423-434.
147. Price, E.M., R.S. Prather, C.M. Foley. 2006. Multipotent adult progenitor cell lines originating from green fluorescent protein transgenic swine. Stem Cells and Development 15:507-522.
148. Hao, Y.H., H.Y. Yong, C.N. Murphy, D. Wax, M. Samuel, A. Rieke, L. Lai, Z. Liu, D.C. Durtschi, V.R. Welbern, E.M. Price, R.M. McAllister, J.R. Turk, M.H. Laughlin, R.S. Prather, E.B. Rucker. 2006. Production of endothelial nitric oxide synthase (eNOS) over-expressing piglets. Transgenic Research 15:739-750.
149. Yi, Y.J., G. Manandhar, M. Sutovsky, R. Li, V. Jonakova, R. Oko, C.S. Park, R.S. Prather, P. Sutovsky. 2007. Ubiquitin C-Terminal hydrolase-activity is involved in sperm acrosomal function and anti-polyspermy defense. Biol. Reprod. 77:780-793.
150. Zhong, Z., L. Spate, Y. Hao, R. Li, L. Lai, M. Katayama, D. Wax, K Whitworth, Q.-Y. Sun, R.S. Prather, H. Schatten. 2007. Remodeling of centrosomes in intraspecies and interspecies nuclear transfer porcine embryos. Cell Cycle 6:1510-1521.
151. Jiang, L., P. Jobst, L. Lai, M. Samuel, D. Ayers, R.S. Prather, X.C. Tian. 2007. Expression of growth-regulating imprinted genes in deceased and surviving cloned pigs. Cloning and Stem Cells 9:97-106.
152. Ross, J.W., M.D. Ashworth, F.J. White, G.A. Johnson, P.J. Ayoubi, U. E. DeSilva, K.M. Whitworth, J.A. Green, R.S. Prather, R.D. Geisert. 2007. Identification and analysis of molecular markers following endocrine disruption of pregnancy in the pig. Endocrinology 148:4761-4773.
153. Bonk, A.J., H.-T. Cheong, R. Li, L. Lai, Y. Hao, Z. Liu, M. Samuel, E.A. Fergason, K.M. Whitworth, C.N. Murphy, E. Antoniou, R.S. Prather. 2007. Correlation of developmental differences to the methylation profiles of nuclear transfer donor cells. Epigenetics 2:3, 179-186.
154. Wu, A.C.H., P. Sutovsky, G. Manandhar, W. Xu, M. Katayama, B.N. Day, Q.-W. Park, R.S. Prather, R. Oko. 2007. PAWP, A sperm specific WW-domain binding protein involved in pronuclear development during mammalian fertilization. Journal of Biological Chemistry 282:12,164-12,175.
155. Isom, S.C., R.S. Prather, E.B. Rucker III. 2007. Heat stress-induced apoptosis in porcine in vitro fertilized and parthenogenetic preimplantation-stage embryos. Mol. Reprod. Dev. 74:574-581.
156. Martin, L.M., C. Besch-Williford, L. Lai, H.-T. Cheong, G.-S. Im, K.-W. Park, C. Murphy, D. Keisler, M. Ellersieck, H. Schatten, J.A. Green, R.S. Prather. 2007. Morphologic and histologic comparisons between in vivo and nuclear transfer derived porcine embryos. Mol. Reprod. Dev. 74:952-960.
157. Im, G.-S., M. Samuel, L. Lai, Y. Hao, R.S. Prather. 2007. Development and calcium level changes in pre-implantation porcine nuclear transfer embryos activated with 6-DMAP after fusion. Mol. Reprod. Dev. 74:1158-1164.
158. Li, R., K. Whitworth, L. Lai, D. Wax, L. Spate, C.N. Murphy, A. Rieke, S.C. Isom, Y. Hao, Z. Zhong, M. Katayama, H. Schatten, R.S. Prather. 2007. Concentration and composition of free amino acids and osmolalities of porcine oviductal and uterine fluid and their effects on development of porcine parthenogenetic and IVF embryos. Mol. Reprod. Dev. 74:1228-1235.
159. Caamaño, J.N., A. Rodriguez, A. Salas, M. Muñoz, C. Diez, R.S. Prather, E. Goméz. 2008. Flow cytometric cell cycle analysis of cultured brown bear fibroblasts. Cell Biology International (accepted).
160. Liu, Z., T. Jiangtian, Z. Zhong, W. Zhenkun, S. Jun, Y. Zhi, G. Li, M. Haikun, K. Qingran, S. Shuang, L. Yutian, W. Hongbin, R.S. Prather. 2008. Green fluorescent protein (GFP) transgenic pig produced by somatic cell nuclear transfer. Chinese Science Bulletin. 53:1035-1039.
161. Rogers, C.S., Y. Hao, T. Rokhlina, M. Samuel, D.A. Stoltz, Y. Li, E. Petroff, D.W. Vermeer, A.C. Kabel, Z. Yan, L. Spate, D. Wax, C.N. Murphy, A. Rieke, K. Whitworth, M.L. Linville, S.W. Korte, J.F. Engelhardt, M.J. Welsh, R.S. Prather. 2008. Production of CFTR null and ΔF508 heterozygous pigs by AAV-mediated gene targeting and somatic cell nuclear transfer. Journal of Clinical Investigation 118:1571-1577.
162. Klassen, H., K. Warfvinge, P.H. Schwartz, J.F. Kiilgaard, N. Shamie, C. Zhang, M. Samuel, E. Scherfig, R.S. Prather, M.J. Young. 2008. Isolation of multiple progenitor cell types from GFP-transgenic pigs and transplantation to the retina of allorecipients. Cloning & Stem Cells 10:391-402.
163. Lorson, M.A., L. Spate, K. Wells, R.S. Prather, C.L. Lorson. 2008. The identification and characterization of the porcine (sus scrofa) survival motor neuron (SMN1) gene. Developmental Dynamics 237:2268-2278.
164. Antelman, J., G. Manandhar, Y.-J. Yi, R. Li, K. Whitworth, J.A. Green, R.S. Prather, P. Sutovsky. 2008. Expression of mitochondrial transcription factor A (TFAM) during porcine gametogenesis and preimplantation embryo development. J. Cellular Physiology. 217:529-543.
165. Zhong, Z., Y. Hao, R. Li, L. Spate, D. Wax, Q.-Y. Sun, R.S. Prather, H. Schatten. 2008. Mitochondria distribution and translocation in cloned porcine embryos: an analysis with fluorescence and transmission electron microscopy. Microsc Microanal. 14:418-432.
166. Bonk, A.J., R. Li, L. Lai, Y. Hao, Z. Liu, M. Samuel, E.A. Fergason, K.M. Whitworth, C.N. Murphy, E. Antoniou, R.S. Prather. 2008. Aberrant DNA methylation in porcine in vitro-, parthenogenetic-, and somatic cell nuclear transfer-produced embryos. Mol. Reprod. Dev. 75:250-264.
167. Jiang, L., L. Lai, M. Samuel, R.S. Prather, X. Yang, X.C. Tian. 2008. Expression of X-linked genes in deceased neonates and surviving cloned female pigs. Mol. Reprod. Dev. 75:265-273.
168. Hao, Y., C. Murphy, L. Spate, D. Wax, Z. Zhong, M. Samuel, N. Mathialagan, H. Schatten, R.S. Prather. 2008. Osteopontin improves in vitro development of porcine embryos and decreases apoptosis. Mol. Reprod. Dev. 75:291-298.
169. McHughes, C.E., G.K. Springer, L. Spate, R. Li, R. Woods, M. Green, S.W. Korte, C.N. Murphy, J.A. Green, R.S. Prather. 2008. Identification and quantification of differentially represented transcripts in preimplantation bovine embryos. Mol. Reprod. Dev. 76:48-60.
170. Rogers, C.S., D.A. Stoltz, D.K. Meyerholz, L.S. Ostedgaard, T. Rokhlina, P. Taft, M.P. Rogan, A.A. Pezzulo, P.H. Karp, O.A. Itani, A.C. Kabel, C.L. Wohlford-Lenane, G.J. Davis, T. L. Smith, M. Samuel, D. Wax, C.N. Murphy, A. Rieke, K. Whitworth, A. Uc, T.D. Starner, K.A. Brogden, J. Shilyansky, P. McCray, Jr., J. Zabner, R.S. Prather, M.J. Welsh. 2008. Disruption of the CFTR gene produces a model of cystic fibrosis in newborn piglets. Science 321:1837-1841.
171. Li, R., C.N. Murphy, L. Spate, D. Wax, S.C. Isom, A. Rieke, E. Walters, M. Samuel, R.S. Prather. 2009. Production of piglets after cryopreservation by using a centrifugation-based method for delipation without micromanipulation. Biology of Reproduction (accepted 11/20/08).
172. Hao, Y., D. Wax, Z. Zhong, C.N. Murphy, L. Spate, M. Samuel, A. Rieke, P. Dyce, J. Li, R.S. Prather. 2009. The potential of the porcine skin-derived stem cells as donors cells for nuclear transfer. Cloning and Stem Cells (accepted 11-25-08).
173. Zhao, M., S.C. Isom, H. Lin, Y. Hao, J. Zhao, J. Whyte, K.B. Dobbs, Y. Zhang, R.S. Prather. 2009. Tracing the stemness of GFP transgenic porcine skin derived progenitors (pSKP) back to specific marker gene expression. Cloning and Stem Cells (accepted 12-8-08).
174. Telugu, B.P.V.L., L. Spate, R.S. Prather, J.A. Green. 2009. Acid peptidase activity released from in vitro produced porcine embryos. Mol. Reprod. Dev. (accepted 8/11/08).
175. Isom, S.C., L. Lai, R.S. Prather, E.B. Rucker III. 2009. Response of porcine cleavage-stage embryos to elevated temperature depends on timing of stressor application relative to oocyte activation. Mol. Reprod. Dev. (accepted 9/5/08).
176. Whitworth, K.M., R. Li, L.D. Spate, D.M. Wax, A. Rieke, J. Whyte, G. Manandhar, J.A. Green, P. Sutovsky, R.S. Prather. 2009. A comparison of three fusion/activation methods used to create enhanced green fluorescent protein pigs by somatic cell nuclear transfer. Mol. Reprod. Dev. (accepted 11/2/08).
177. Isom, S.C., R.S. Prather, E.B. Rucker, III. The enhanced developmental potential of heat stressed porcine parthenogenetic embryos is associated with accelerated inactivation of the MAP kinase signaling cascade. (submitted Reproduction, Fertility and Development).
178. Powell, M.D., G. Manandhar, L. Spate, M. Sutovsky, S. Zimmerman, S. Sachdev, M. Hannink, J.A. Green, R.S. Prather, P. Sutovsky. Discovery of putative oocyte quality markers by comparative ExacTag proteomics. (in preparation).
179. Richards, M.P., P. Kathirvel, Y. Gong, E. Walters, R.S. Prather. Effect of genetically enhancing long chain n-3 fatty acid levels in loin muscle on lipid oxidation during frozen storage (in preparation).
180. Isom, S.C., R.S. Prather. Evaluation of relative transcript abundances in porcine preimplantation-stage embryos of different developmental capacities. (in preparation).

Peer-Reviewed /Invited Review Papers

1. Prather, R.S., L. Hagemann, N.L. First. 1989. Preimplantation mammalian aggregation and injection chimeras. Gamete Res. 22:233-247.
2. Prather, R.S. 1990. Nuclear transplantation as a method for cloning embryos. Proc. Soc. Exp. Biol. & Med. 195:7-12.
3. Prather, R.S. 1991. Nuclear transplantation and embryo cloning in mammals. ILAR News 33:62-68.
4. First, N.L., R.S. Prather. 1991. Genomic potential in mammals. Differentiation 48:1-8.
5. Prather, R.S., J.M. Robl. 1991. Cloning by nuclear transfer and splitting in laboratory and domestic animals. In: Animal Applications of Research in Mammalian Development (R.A. Pedersen, A. McLaren, N.L. First, eds.) The Cold Spring Harbor Laboratory Press, pp 205-232.
6. Prather, R.S., T.T. Stumpf and L.F. Rickords. 1992. Nuclear transplantation as a method of producing genetically identical livestock. Animal Biotechnology 3:67-79.
7. Prather, R.S. 1996. Progress in cloning mammalian embryos. Proc. Soc. Exp. Biol. & Med. 212:38-43.
8. Prather, R.S., B.N. Day. 1998. Practical aspects of in vitro production of pig embryos. Theriogenology 49:23-32.
9. Macháty, Z., R.S. Prather. 1998. Strategies for activating nuclear transfer oocytes. Reproduction, Fertility & Development. 10:599-613.
10. Prather, R.S., T. Tao, Z. Macháty. 1999. Development of the techniques for nuclear transfer in pigs. Theriogenology 51:487-498.
11. Kűhholzer, B. and R.S. Prather. 2000. Advances in livestock nuclear transfer. Proc. Soc. Exp. Biol. & Med. 224:240-245.
12. Prather, R.S., B. Kűhholzer, L. Lai and K.-W. Park. 2000. Changes in the structure of nuclei after transfer to oocytes. Cloning 2:117-122.
13. Prather, R.S. 2001. Basic mechanisms of fertilization and parthenogenesis in the pig. Reproduction Supplement 58: 105-112.
14. Lai, L., R.S. Prather. 2002. Progress in producing knockout pigs for xenotransplantation by nuclear transfer. Annals of Medicine 34:501-506.
15. Prather, R.S., R.J. Hawley, D.B. Carter, L. Lai, J.L. Greenstein. 2003. Transgenic swine for biomedicine and agriculture. Theriogenology 59:115-125.
16. Lai, L. and R.S. Prather. 2003. Genetic modification of pigs by nuclear transfer. Repro. Biol. & Endo. 1:82-87. http://www.rbej.com/content/1/1/82
17. Lai, L., R.S. Prather. 2003. Production of cloned pigs by using somatic cells as donors. Cloning & Stem Cells 5:233-242.
18. Whitworth, K., G.K. Springer, L.J. Forrester, W.G. Spollen, J. Ries, C.N. Murphy, N. Mathialagan, B.A. Didion, J.A. Green, R.S. Prather. 2003. Characterization of cDNA Libraries Developed from Porcine Oocytes and Embryos to Determine Differential Expression Patterns. In: Mammalian Embryo Genomics, Organization for Economic Co-Operation and Development, Paris, France, pp 29-36.
19. Holt, W.V., A.R. Pickard, R.S. Prather. 2004. Wildlife conservation and reproductive cloning. Reproduction 127:317-324.
20. Platt, J.L., R.S. Prather. 2004. Animal organ donors- human health applications. CAST Issue Paper 26:1-8.
21. Lai, L., R.S. Prather. 2004. Cloning pigs as donors for organ transfers in humans: promises and challenges. IEEE Engineering in Medicine and Biology. 23:37-42.
22. Lai, L., R.S. Prather. 2004. Biotechnology: Cloning animals. In: Encyclopedia of Animal Science (W.G. Pond and A.W. Bell, Eds), Marcel Dekker, New York, NY.
23. Prather, R.S., P. Sutovsky, J.A. Green. 2004. Nuclear remodeling and reprogramming in transgenic pig production. Soc. Exp. Biol. & Med. 229:1120-1126.
24. Schatten, H., R.S. Prather, Q.-Y. Sun. 2005. The significance of mitochondria for embryo development in cloned farm animals. Mitochondrion 5:303-321.
25. Green, J.A., J.-G. Kim, K.M. Whitworth, C. Agca, R.S. Prather. 2006. The use of microarrays to define functionally-related genes that are differentially expressed in the cycling pig uterus. In: Control of Pig Reproduction VII, Reproduction Supplement 62. Eds C.J. Ashworth and R. Kraeling. Nottingham University Press, Nottingham, UK. pp 163-176.
26. Prather, R.S. 2006. Cloned transgenic heart-healthy pork? Transgenic Research 15: 405-407.
27. Rogers, C.S., W.M. Abraham, K.A. Brogden, J.F. Engelhardt, J. fisher, P.B. McCray, G. McLennan, D.K. Meyerholz, L.S. Ostedgaard, R.S. Prather, D.A. Stolz, J. Zabner, M.J. Welsh. 2008. The porcine lung as a potential model for cystic fibrosis. Am. J. Phys. Lung Cell. Mol. Physiol. 295:L240-L263.
28. Prather, R.S., M. Shen, Y. Dai. 2008. Genetically modified pigs for medicine and agriculture. Biotechnology & Genetic Engineering Reviews 25:245-266.
29. Prather, R.S., J.W. Ross, S.C. Isom, J.A. Green. 2009. Transcriptional, post-transcriptional and epigenetic control of porcine oocyte maturation and embryogenesis. International Conference on Pig Reproduction (in preparation).
30. Ross, J.W., R.S. Prather, J.J. Whyte, J. Zhao. 2009. Cloning and transgenics: new approaches. CAB Reviews (in preparation).

Other Scientific Correspondence

1. Schatten, G., R. Prather, I. Wilmut. 2003. Cloning claim is science fiction, not science. Science 299:344.
2. Prather, R.S. 2007. Targeted genetic modification: xenotransplantation and beyond. Cloning and Stem Cells 9:17-19.

Invited Review Papers and Book Chapters

1. Prather, R.S., F.L. Barnes, and N.L. First.1987. Nuclear transfer in the domestic species. In: Application of egg- and embryo technologies to domestic animals. Department of Obstetrics and Gynecology, The Royale Veterinary and Agricultural University, Copenhagen, Denmark pp 27-29.
2. First, N.L. and R.S. Prather. 1987. Advances in animal biotechnology. International Symposium on Genetics for the Biological Efficiency of Production, Aug. 24-28, 1987. Piracicaba Brazil.
3. Prather, R.S. and G. Schatten. 1988. Studies in Fertilization. Proc. 12th NAAB Tech. Conf. on A.I. and Reprod. pp. 94-96.
4. Prather, R.S., N.L. First 1988. Nuclear transplantation in early mammalian embryos. In: The American Fertility Society Regional Postgraduate Course. Hands-on IVF, Cryopreservation and Micromanipulation, April 25-29 Madison, Wisconsin pp. 53-61.
5. Prather, R.S. 1989. Nuclear transplantation in mammals and amphibians: nuclear equivalence, species specificity? In: The Molecular Biology of Fertilization (H. Schatten, G. Schatten eds.), Academic Press, Orlando. pp 323-340.
6. Stricker, S., R. Prather, C. Simerly, H. Schatten and G. Schatten. 1989. Nuclear architectural changes during fertilization and development. In: The Cellular Biology of Fertilization (H. Schatten, G. Schatten, eds.), Academic Press, Orlando. pp 225-250.
7. Prather, R.S. and N.L. First. 1990. Cloning embryos by nuclear transfer. 2nd Symposium on Genetic Engineering of Animals, Ithaca, NY. J. Reprod. Fert. Suppl. 41:125-143.
8. Prather, R.S. and N.L. First. 1990. Nuclear Transfer. In: Advances in in vitro Fertilization and Assisted Reproductive Technologies (S. Mashiach, Z. Ben-Rafael, N. Laufer, J.G. Schenker ed). Plenum Press, New York. pp. 929-938.
9. Prather, R.S. and N.L. First.1990. The cloning of embryos. 3rd International Conference on Pig Reproduction. J. Reprod. Fert. Suppl. 40:227-234.
10. Prather, R.S. and N.L. First. 1990. Nuclear transfer in mammals. Int. Rev. Cytol. 120:169-190.
11. First, N.L. and R.S. Prather. 1991. Production of embryos by oocyte cytoplast-blastomere fusion in domestic animals. 3rd International Ruminant Reproduction Symposium, Nice, France. J. Reprod. Fert. Suppl. 43:245-254.
12. Prather, R.S. 1993. Nuclear control of preimplantation development in the domestic pig. 4th International Conference on Pig Reproduction. J. Reprod. Fert. Suppl. 48:17-29.
13. Robl, J.M., C.R. Long, R.S. Prather and R.T. Duby. 1995. Non-rodent transgenic strategies. In: Strategies for Transgenic Animals (G Monastersky & JM Robl, ed). American Society for Microbiology 57-72.
14. Prather, R.S., J.-H. Kim, Z. Machàty. 1997. Artificial activation of pig oocytes arrested at meiotic metaphase II. Korean Society of Animal Reproduction. Korean J. Anim. Reprod. 20:395-412.
15. Prather, R.S. 1998. Cloning mammals by nuclear transfer. In: Encyclopedia of Reproduction (E. Knobil & J.D. Neill, ed). Academic Press Volume 1: 638-644.
16. Prather, R.S. 1999. Cloning and transgenic animal production: A technological revolution. Institute for Theological Encounter with Science and Technology. Loyola University, Chicago, IL Aug 1-5.
17. Day, B.N., L.R. Abeydeera and R.S. Prather. 2000. Recent progress in pig embryo production through in vitro maturation and fertilization techniques. In: LA Johnson & HD Guthrie (ed), Boar Semen Preservation IV, Allen Press, Kansas 81-92.
18. Prather, R.S. 2000. Perspectives: Cloning. Pigs is pigs. Science 289:1886-1887.
19. Prather, R.S. 2000. Nuclear modifications and reprogramming after nuclear transfer. In: Contemporary Endocrinology: Assisted Reproduction and Nuclear Transfer in Mammals (D.P. Wolf & M. Zelinski-Wooten, ed). Humana Press p227-238.
20. Tuggle, C.K., R.S. Prather, M.B. Soares, T. Casavant, D. Pomp, M.F. Rothschild, W. Beavis. 2001. Gene discovery and functional genomics in the pig. National Swine Improvement Federation Proceedings. http://www.nsif.com/01proc/tuggle.htm
21. Prather, R.S. 2002. Nuclear transfer in swine. In: Principles of Cloning (J.B. Cibelli, R. Lanza, K. Campbell & M.D. West, ed). Academic Press p 367-374.
22. Critser, J.K., L.K. Riley and R.S. Prather. 2003. Application of nuclear transfer technology to conservation. In: Reproduction and Integrated Conservation Science Eds. W.V. Holt, A.R. Pickard, J.C. Roger & D. E. Wildt. Cambridge University Press. Cambridge, UK. p 195-208.
23. Im, G.-I. and R.S. Prather. 2003. Genetic modification in swine for biomedicine and agriculture. Korea Conference on Innovative Science and Technology. p. 61-72.
24. Prather, R.S. and D. Gerrard. 2003. Use of cloning and transgenesis in pigs. Reciprocal Meat Conference Proceedings: Columbia, Missouri. Reciprocal Meat Conference.
25. Lai, L. and R.S Prather. 2004. Creation of transgenic pigs by nuclear transfer. In: Methods in Molecular Medicine (H. Schatten, ed). Humana Press pp 149-164.
26. Prather, R.S. 2004. Genetic modification of swine for medicine and agriculture. Proceedings of the American Embryo Transfer Association. Tampa, FL. October 14-16.
27. Prather, R.S. 2005. Transgenic approaches to developing swine models. American Association of Laboratory Animal Sciences annual meeting (proceedings published by Sinclair Research Center, Columbia, MO). pp 11-13.
28. Prather, R. S. and M.F. Smith. 2006. Reproductive Biotechnologies. In: Biotechnology Patent Law Technology, R. Brungs & M. Postiglione, eds. ITEST Faith Science Press, St. Louis, MO. pp 1-13.
29. Dai, Y. and R.S. Prather. 2006. Omega-3 pigs. ISB News Report http://www.isb.vt.edu/news/2006/news06.jun.htm#jun0604 .
30. Prather, R.S. 2006. Nuclear Remodeling and Nuclear Reprogramming for Making Transgenic Pigs by Nuclear Transfer. In: Somatic Cell Nuclear Transfer (Peter Sutovsky, ed.), Landes Bioscience, Georgetown, TX. pp 1-13.
31. Lai, L. and R.S. Prather. 2007. Animal Cloning. In: Comparative Reproductive Biology (H. Schatten and G.M. Constantinescu, eds.) Blackwell Publishing, Ames, Iowa. Pp 237-262.
32. Critser, J.K., M.H. Laughlin, R.S. Prather, L.K. Riley. 2008. Proceedings of The Conference on Swine in Biomedical Research. ILAR News (in press).

88,042 Genome Sequence Data Base Submissions (GenBank)

L47772 Anderson, J.A., R.L. Matteri, R.S. Prather. 1995. Partial cDNA encoding a TCP-1 related protein expressed in day 17 porcine embryo.
L48205 Anderson, J.A., R.L. Matteri, R.S. Prather. 1995. Partial cDNA encoding a porcine cyclin B homologue.
U58370 Matteri, R.L., J.A. Anderson, R.S. Prather. 1996. Partial cDNA sequence of the porcine IGF-I receptor.
U58650 Matteri, R.L., J.A. Anderson, R.S. Prather. 1996. Partial cDNA sequence of the porcine IGF-II receptor.
AF124218 Ruddock, N.T., R. L. Matteri, R.S. Prather. 1999. Na+/H+ exchanger, isoform 4 (NHE4).
AF123280 Ruddock, N.T., R. L. Matteri, R.S. Prather. 1999. cDNA sequence of Na+/H+ exchanger, isoform 3 (NHE3).
AF184171 Ruddock, N.T., R. L. Matteri, R.S. Prather. 1999. cDNA sequence of Na+/H+ exchanger, isoform 2.
AF210153 Cabot, R.A., R.L. Matteri, R.S. Prather. 1999. Sus scrofa nuclear export factor CRM1 mRNA, partical cds.
AF260664 Ruddock, N.T., R.L. Matteri, R.S. Prather. 2000. Sus scrofa NHE5, sodium-proton exchanger, isoform 5 mRNA, partial cds.
AY010068 VanVickle, S.J., R.A. Cabot, R.S. Prather. 2000. Porcine karyopherin (importin) alpha 6 (Kpna6) mRNA from cDNA clone, partial cds.
AY010067 VanVickle, S.J., R.A. Cabot, R.S. Prather. 2000. Porcine karyopherin (importin) alpha 4 (Kpna4) mRNA from cDNA clone, partial cds.
AY010069 VanVickle, S.J., R.A. Cabot, R.S. Prather. 2000. Porcine karyopherin (importin) alpha 3 (Kpna3) mRNA from cDNA clone, partial cds.
AY010065 VanVickle, S.J., R.A. Cabot, R.S. Prather. 2000. Porcine karyopherin (importin) alpha 2 (Kpna2) mRNA from cDNA clone, partial cds.
AY010066 VanVickle, S.J., R.A. Cabot, R.S. Prather. 2000. Porcine karyopherin (importin) alpha 1 (Kpna1) mRNA from cDNA clone, partial cds.
BF701840-704551 Tuggle, C., R. Woods, J.A. Green, R.S. Prather. 2000. USDA funded EST Project. Sus scrofa cDNAs. 2,712 sequences submitted.
BF711266-713604 Tuggle, C., R. Woods, J.A. Green, R.S. Prather. 2001. USDA funded EST Project. Sus scrofa cDNAs. 2,339 sequences submitted.
AY034475-034477 Wei, Z., J.A. Green, R.S. Prather. 2001. Sus Scrofa embryonic proteinase inhibitor 1, 2, and 3 (EPI-1, EPI-2, EPI-3), respectively.
AF421152 Cabot, R.A., R.S. Prather. 2001. Porcine karyopherin (importin) alpha 5 (Kpna5) mRNA from cDNA clone, partial cds.
BG322266-322271 Tuggle, C., R. Woods, J.A. Green, R.S. Prather. 2001. USDA funded EST Project. Sus scrofa cDNAs. 46 sequences submitted.
BI404564-405235 Tuggle, C., R. Woods, J.A. Green, R.S. Prather. 2001. USDA funded EST Project. Sus scrofa cDNAs. 672 sequences submitted.
AF420483 Machaty, Z., J.J. Ramsoondar, A.J. Bonk, K.R. Bondioli, R.S. Prather. 2002. Sus scrofa oocyte transient receptor potential channel mRNA, partial cds.
AF535216 Rucker,E.B. III, G. Cooper, D. Durtschi, R.S. Prather, M.H. Laughlin. 2002. Endothelial NOS from Yucatan Pig Genomic DNA.
CK726476-730336 Prather, R.S., Antoniou E., Garverick H.A., Green J.A., Lucy M.C., Roberts R.M., Smith M.F., Youngquist R.S. 2004. USDA Grant NRI-2002-03476: Bovine ESTs: Focus on Female Reproduction. 3,861 sequences.
CK778935-780235 Prather, R.S., Antoniou E., Garverick H.A., Green J.A., Lucy M.C., Roberts R.M., Smith M.F., Youngquist R.S. 2004. USDA Grant NRI-2002-03476: Bovine ESTs: Focus on Female Reproduction. 1,301 sequences.
CK816249-817253 Prather, R.S., Antoniou E., Garverick H.A., Green J.A., Lucy M.C., Roberts R.M., Smith M.F., Youngquist R.S. 2004. USDA Grant NRI-2002-03476: Bovine ESTs: Focus on Female Reproduction. 1,005 sequences.
CK983044-983154 Prather, R.S., Antoniou E., Garverick H.A., Green J.A., Lucy M.C., Roberts R.M., Smith M.F., Youngquist R.S. 2004. USDA Grant NRI-2002-03476: Bovine ESTs: Focus on Female Reproduction. 111 sequences.
CN024942-033007 Whitworth, K., G.K. Springer, L.J. Forrester, W.G. Spollen, J. Ries, W.R. Lamberson, N. Bivens, C.N. Murphy, N. Mathialagan, J.A. Green, R.S. Prather. 2004. Developmental expression of 2,489 genes during pig embryogenesis: An EST project. 8,066 sequences.
CN653841-654882 Prather, R.S., Antoniou E., Garverick H.A., Green J.A., Lucy M.C., Roberts R.M., Smith M.F., Youngquist R.S. 2004. USDA Grant NRI-2002-03476: Bovine ESTs: Focus on Female Reproduction. 1,042 sequences.
CN997955-CO000943 Prather, R.S., Antoniou E., Garverick H.A., Green J.A., Lucy M.C., Roberts R.M., Smith M.F., Youngquist R.S. 2004. USDA Grant NRI-2002-03476: Bovine ESTs: Focus on Female Reproduction. 2,988 sequences.
CO727060-729165 Prather, R.S., Antoniou E., Garverick H.A., Green J.A., Lucy M.C., Roberts R.M., Smith M.F., Youngquist R.S. 2004. USDA Grant NRI-2002-03476: Bovine ESTs: Focus on Female Reproduction. 2,106 sequences.
CO936965-948408 Whitworth, K.M., Springer, G.K., Bivens, N.J., Ries, J.E., Woods, R.J., Spollen, W.G., Forrester, L.J., Mathialagan, N., Prather, R.S., Green, J.A. 2004. Large-scale Generation and Analysis of Expressed Sequence Tags from porcine conceptuses and fetuses. 11,444 sequences.
CO948409-956968 Jiang, H., Whitworth, K.M., Bivens, N.J., Ries, J.E., Woods, R.J., Forrester, L.J., Springer, G.K., Mathialagan, N., Agca, C., Prather, R.S., Lucy, M.C. 2004. Large-scale Generation and Analysis of Expressed Sequence Tags from Porcine Ovary. 8,560 sequences.
CO986156-995036 Kim, J., Whitworth, K.M., Springer, G.K., Bivens, N.J., Ries, J.E., Woods, R.J., Spollen, W.G., Forrester, L.J., Mathialagan, N., Prather, R.S., Green, J.A. 2004. Large-scale Generation and Analysis of Expressed Sequence Tags from Porcine endometrium and oviduct. 8,881 sequences.
CV100863-102245 Woods, R.J., Whitworth, K.M., Springer, G.K., Forrester, L.J., Spollen, W.G., Ries, J.E., Bivens, N.J., Murphy, C.N., Mathialagan, N., Agca, C., Green, J.A., Prather, R.S. 2004. Large-scale Generation and Analysis of Expressed Sequence Tags from Porcine Cryopreserved Blastocyst Stage Embryos. 1,383 sequences.
CV974020-984840 Prather, R.S., Antoniou E., Garverick H.A., Green J.A., Lucy M.C., Roberts R.M., Smith M.F., Youngquist R.S. 2004. USDA Grant NRI-2002-03476: Bovine ESTs: Focus on Female Reproduction. 10,821 sequences.
CX948071-955444 Prather, R.S., Antoniou E., Garverick H.A., Green J.A., Lucy M.C., Roberts R.M., Smith M.F., Youngquist R.S. 2005. USDA Grant NRI-2002-03476: Bovine ESTs: Focus on Female Reproduction. 7,374 sequences.
DD1177680-1177687 Day, B.N., Prather, R.S., Hawley, R.J., 2005. Knockout swine and method for making the same. 9 sequences.
DN636932-643531 Prather, R.S., Antoniou E., Garverick H.A., Green J.A., Lucy M.C., Roberts R.M., Smith M.F., Youngquist R.S. 2005. USDA Grant NRI-2002-03476: Bovine ESTs: Focus on Female Reproduction. 6,600 sequences.
DN818238-823872 Prather, R.S., Antoniou E., Garverick H.A., Green J.A., Lucy M.C., Roberts R.M., Smith M.F., Youngquist R.S. 2005. USDA Grant NRI-2002-03476: Bovine ESTs: Focus on Female Reproduction. 5,635 sequences.
DN984173-985068 Prather, R.S., Antoniou E., Garverick H.A., Green J.A., Lucy M.C., Roberts R.M., Smith M.F., Youngquist R.S. 2005. USDA Grant NRI-2002-03476: Bovine ESTs: Focus on Female Reproduction. 896 sequences.
DQ011859 Manandhar, G., Feng, D, Yi, Y.J, Lai, L., Letko, J., Laurincik, J., Sutovsky, M., Prather, R.S., Schatten, H., Sutovsky, P. 2005. Sus scrofa centrin 2 mRNA, partial cds.
DQ066552 Manandhar, G., Feng, D, Yi, Y.J, Lai, L., Letko, J., Laurincik, J., Sutovsky, M., Salisbury, J.L., Prather, R.S., Schatten, H., Sutovsky, P. 2005. Sus scrofa centrin 3 mRNA, partial cds.
DR749126-749203 Prather, R.S., Antoniou E., Garverick H.A., Green J.A., Lucy M.C., Roberts R.M., Smith M.F., Youngquist R.S. 2005. USDA Grant NRI-2002-03476: Bovine ESTs: Focus on Female Reproduction. 78 sequences.
DQ441590 McHughes, C.E., Rucker, E.B. III, Prather, R.S., Welbern, V.R. 2006. Sus scrofa sialoadhesin (SN) gene, exons 1 through 7 and partial cds.
DQ915200-915250 Prather, R., A.J. Bonk, E.A. Fergason, S.C. Isom. 2006. Sus scrofa CpG methylation island genomic sequence. 51 sequences.
EF185170-185205 Prather, R., A.J. Bonk, E.A. Fergason, S.C. Isom. 2006. Sus scrofa CpG methylation island genomic sequence. 36 sequences.
EF189709-189710 Prather, R., A.J. Bonk, E.A. Fergason, S.C. Isom. 2006. Sus scrofa CpG methylation island genomic sequence. 2 sequences.

Extension Publications

1. Allee, G.L., Po-Heng Hsu and R.S. Prather. 1981. Soybean protein in milk replacer diets for baby pigs. Swine Day Report of Progress. 406, Ks. Ag. Exp. Station. pp. 5-7.
2. Prather, R.S. and M.S. Peters. 1990. Cloning cattle by nuclear transfer. 21st Annual University of Missouri Beef Cow-Calf Clinic, July 24. pp. 38-40.
3. Prather, R.S., A. Rieke, B.N. Day. 1997. Non-surgical embryo transfer in pigs: introduction of new animals with little risk of disease transmission. Swine Reproduction Symposium. University of Missouri-Columbia. October 20-21, 1997.
4. Prather, R.S., A. Rieke, B.N. Day. 1997. Non-surgical embryo transfer in pigs: introduction of new animals with little risk of disease transmission. A.I.- A tool for profitable pork production. University of Missouri-Columbia. March 10-11,1997.
5. Prather, R.S. 1997. Summary of research program on early development of pig embryos. Animal Sciences Departmental Report, University of Missouri-Columbia 59-61.
6. Prather, R.S. and B.N. Day. 1998. Development of technologies for the production and manipulation of early pig embryos. Animal Sciences Departmental Report. University of Missouri-Columbia pp 37-39.
7. Prather, R.S. A. Rieke, B.N. Day. 1998. Non-surgical embryo transfer in pigs: introduction of new genetics with little risk of disease transmission. Animal Sciences Departmental Report. University of Missouri-Columbia pp 43-45.
8. Prather, R.S., A. Rieke, B.N. Day. 1998. Non-surgical embryo transfer in pigs: Introduction of new genetics with little risk of disease transmission. A.I.-A tool for profitable pork production. University of Missouri-Columbia. March 2-3, 1998.
9. Macháty, Z., B.N. Day, R.S. Prather. 1999. Development of early pig embryos in vitro and in vivo. Animal Sciences Departmental Report. University of Missouri-Columbia. pp 139-142.
10. Perry, G.A. R.S. Prather, M.F. Smith. 1999. In vitro meiotic competence of oocytes collected from porcine antral follicles. Animal Sciences Departmental Report. University of Missouri-Columbia. pp 143-147.
11. Abeydeera, L.R., R.S. Prather, B.N. Day. 1999. Epidermal growth factor can increase the developmental competence of pig oocytes. Animal Sciences Departmental Report. University of Missouri-Columbia. pp 148-150.
12. Tuggle, C.K., S. Malchenko, R. Woods, K. Whitworth, J.A. Green, R. Prather, C.A. Roberts, C.J. Fitzsimmons, T. Casavant, M.B. Soares. 2000. Development of new placental and fetal ESTs for gene discovery in pig production, ISU Swine Research Report. ASL-R668, pp104-107.
13. Shi, X.W., C.J. Fitzsimmons, C.K. Tuggle. C. Genet, R. Prather, K. Whitworth, J.A. Green. 2000. High-resolution radiation hybrid mapping of human chromosome 17 genes to the pig genome extends gene order conservation with pig chromosome 12 and limited synteny with pig chromosome 2, ISU Swine Research Report. ASL-R668, pp 96-100.
14. Macháty, Z., A.J. Bonk, B. Kuhholzer, R.S. Prather. 2000. Activation of pig eggs induced by an extract from boar sperm. Animal Sciences Departmental Report. University of Missouri-Columbia.
15. Jiang, H., N.J. Bivens, J.E. Ries, K.M. Whitworth, J.A. Green, L.J. Forrester, G.K. Springer, , R.S. Prather and M.C. Lucy. 2002. Constructing cDNA libraries with fewer clones that contain long poly(dA) tails. University of Missouri Molecular Biology Newsletter March/April pp 4-5.
16. Prather, R.S. 2002. Transgenic pigs. Q.A. Science, Euromedia Associates LTD.
17. Prather, R. S. 2004. Genetically Modified Pigs: Medicine and Agriculture. Missouri Pork Association's annual magazine.

Abstracts

1. Prather, R.S., M.F. Spire. 1984. The effect of hydrogen ion concentration on the freeze-thaw survival of mouse embryos. Theriogenology 21:255.
2. Prather, R.S., M.F. Spire, R.R. Schalles.1984. Incorporation of norgestomet into a superovulation regime. Theriogenology 21:256.
3. Prather, R.S., N.L. First. 1985. Effect of cytochalasin B and demecolcine on freeze-thaw survival of murine embryos. Cryobiology 22(6):619.
4. Prather, R.S., N.L. First. 1985. Cryopreservation of isolated murine blastomeres. Theriogenology 23:219.
5. Prather, R.S., F.L. Barnes, J.M. Robl, N.L. First. 1986. Multiplication of bovine embryos. Biol. Reprod. 34 Suppl (1): 192.
6. Prather, R.S., N.L. First. 1986. Developmental fate of highly asynchronous chimeric mouse embryos. Theriogenology 25:185.
7. Robl, J.M., R. Prather, W. Eyestone, F. Barnes, D. Northey, B. Gilligan, N.L. First. 1986. Nuclear transplantation in bovine embryos. Theriogenology 25:189.
8. Barnes, F.L., R.S. Prather, J.M. Robl, N.L. First. 1987. Multiplication of bovine embryos. Theriogenology 27:209.
9. Prather, R.S., N. First, G. Maul, G. Schatten. 1988. Distribution of and changes in nuclear lamin antigens during early bovine and porcine embryogenesis. 4th International Congress of Cell Biology. Montreal, Canada, Aug.14-19. p 413.
10. Prather, R.S., G.G. Maul N. Chaly, D.L. Brown, G. Schatten. 1988. Construction of the nuclear matrix during early murine embryogenesis and its alpha-amanitin sensitivity. 4th International Congress of Cell Biology. Montreal, Canada, Aug.14-19. p 328.
11. Howard, C., G. Coffe, H. Schatten, M. Walter, H. Biessman, R. Prather, G. Schatten.1988. Isolation and characterization of the sea urchin centrosome. 4th International Congress of Cell Biology. Montreal, Canada, Aug. 14-19. p 146.
12. Prather, R.S., M.L. Sims, N.L. First 1988. Nuclear transplantation in the porcine embryo. Theriogenology 29:290.
13. Hagen, D.R., R.S. Prather, N.L. First.1989. Electrical activation of in vitro matured pig oocytes. J. Anim. Sci. 72 Suppl(1):376.
14. Hagen, D.R., R.S. Prather, M.M. Sims, N.L. First. 1989. Development of pig embryos to the blastocyst (BL) in sheep and pig oviducts and co-culture in vitro. J. Anim. Sci. 72 Suppl(1):209-210.
15. Prather, R.S., M.M. Sims, N.L. First. 1989. Nuclear transfer in pig embryos. 3rd International Conference on Pig Reproduction. University of Nottingham, U.K. April 11-14. p 43.
16. First, N.L., R.S. Prather. 1989. Nuclear Transfer. 6th World Congress, In vitro Fertilization and Alternate Assisted Reproduction, Jerusalem, Israel.
17. Prather, R.S., N.L. First. 1989. Cloning embryos by nuclear transfer. Second Symposium on Genetic Engineering in Animals. Cornell University, Ithaca, NY, June 25-28.
18. Jarrell, V.L., B.N. Day, R.S. Prather. 1990. Synthesis of protein in pig oocytes and embryos. Biol. Reprod. 42 Suppl(1):53.
19. Nicks, D.K., R.S. Prather. 1991. Methionine transport in pig oocytes and early blastocysts. Biol. Reprod. 44 Suppl(1): 155.
20. Prather, R.S., P.A. Eichen, D.K. Nicks, M.S. Peters. 1991. Activation of porcine oocytes matured in vitro. Theriogenology 35:258.
21. Schoenbeck, R.A., M.S. Peters, L.F. Rickords, R.S. Prather. 1992. Combined second messenger induced activation of porcine oocytes. Biol. Reprod. Suppl(1):81.
22. Rickords, L.F., M.S. Peters, T.T. Stumpf, R.S. Prather. 1992. Effect of the protein kinase C inhibitor staurosporine on oocyte activation of in vitro matured porcine oocytes. Biol. Reprod. Suppl(1):82.
23. Stumpf, T.T., R.A. Schoenbeck, R.S. Prather. 1992. DNA synthesis during the porcine embryo two-cell stage. Biol. Reprod. Suppl(1):71.
24. Schoenbeck, R.A., R.S. Prather. 1992. Second messenger enhanced activation of porcine oocytes. Midwest Animal Science Meetings. Des Moines, IA. p 74.
25. Boice, M.L., M.F. Smith, R.S. Prather, B.N. Day. 1992. Hormonal control of bovine oviductal glycoproteins. Midwest Animal Science Meetings. Des Moines, IA. p 78.
26. Prather, R.S., T.T. Stumpf, L.F. Rickords. 1992. Reprogramming the nucleus and synchronizing it with the cytoplasm. International Symposium on: 'Cloning Mammals by Nuclear Transplantation', Fort Collins, CO, January 15, 1992. pp 26-27.
27. Boice, M.L., P.A. Mavrogianis, C.N. Murphy, H.G. Verhage, R.S. Prather, B.N. Day. 1992. Immunocytochemical analysis of the association of bovine oviduct-specific secretory glycoproteins with early embryos. Theriogenology 37:194.
28. Terlouw, S.L., R.S. Prather, B.N. Day.1992. In vitro development of nuclear transplant pig embryos. Theriogenology 37:194.
29. Stumpf, T.T., S.L. Terlouw, H. Funahashi, R.S. Prather, B.N. Day. 1993. Premature chromosomal condensation of nuclei transplanted into in vitro matured porcine oocytes. Biol. Reprod. Suppl 48:175.
30. Stumpf, T.T., S.L. Terlouw, H. Funahashi, R.S. Prather, B.N. Day. 1993. Processing of nuclei transplanted into in vitro matured porcine oocytes. Theriogenology 39:322.
31. Rickords, L.F., M.S. Peters, R.A. Schoenbeck, T.T. Stumpf, R.S. Prather. 1993. Okadaic acid increases rate of activation of electrically activated in vitro matured porcine oocytes. Theriogenology 39:296.
32. Schoenbeck, R.A., M.S. Peters, L.F. Rickords, T.T. Stumpf, R.S. Prather. 1993. DNA synthesis and the transition from maternal to embryonic control in the 4-cell porcine embryo. Theriogenology 39:305.
33. Terlouw, S.L., T.T Stumpf, H. Funahashi, R.S. Prather, B.N. Day. 1993. Pig oocyte activation and processing of transplanted nuclei. Theriogenology 39:329.
34. Mayes, M.A., P.L. Stogsdill, T.W. Parry, D.A. Kinden, R.S. Prather. 1994. Reprogramming of nucleoli after nuclear transfer of pig blastomeres into enucleated oocytes. Dev. Biol. 163:542.
35. Parry, T.W., R.S. Prather.1994. Protein reprogramming after nuclear transfer? J. Anim. Sci. 72(suppl 1): 175.
36. Jolliff, W.J., R.S. Prather. 1994. Parthenogenetic development of in vitro matured porcine oocytes to blastocyst. Biol. Reprod. 50: Suppl (1)125.
37. Nussbaum, D.J., R.S. Prather. 1994. Effects of protein synthesis inhibition on porcine oocytes. J. Anim. Sci. 72(suppl 2):74.
38. Rickords, L.F., R.S. Prather, D.J. Kovar. 1995. Staurosporine induces pronuclear formation but does not elicit a rise in intracellular calcium in mouse oocytes. Biol. Reprod. 52: (Suppl 1)470.
39. Funahashi, F., Z. Machaty, R.S. Prather, B.N. Day. 1995. g-GIutamyltranspeptidase (GTT) of spermatoza may reduce oocyte glutathione (GSH) content at sperm penetration. Biol. Reprod. 52: (Suppl 1)334.
40. Machàty, Z., M.A. Mayes, R.S. Prather. 1995. Effects of injecting calcium into porcine oocytes. Biol. Reprod. 52: (Suppl 1)260.
41. Li, J., A. Rieke, B.N. Day, R.S. Prather. 1995. Technical breakthrough in non-surgical porcine embryo transfer. Biol. Reprod. 52: (Suppl 1)281.
42. Kim, N.H., H. Funahashi, R.S. Prather and B.N. Day. 1995. Cytoskeletal reorganization in porcine oocytes after chemical activation. Midwest Animal Science Meetings.
43. Kim, N.H., R.S. Prather, G. Schatten and B.N. Day. 1995. Microtubule and microfilament dynamics of porcine oocytes during in vitro maturation. Theriogenology 43:249.
44. Machàty, Z., M.A. Mayes, R.S. Prather. 1995. Activation of the G protein in porcine oocytes. Theriogenology 43:271.
45. Mayes, M.A., C.N. Murphy, R.S. Prather. 1996. Parthenogenetic activation of pig oocytes by extended exposure to low levels of broad spectrum protein kinase inhibitors. Biol. Reprod. 54: (Suppl 1)71.
46. Li, J., L.R. Abeydeera, R.L. Matteri, B.N. Day, R.S. Prather. 1996. Transcriptional activities of IGF-2, IGF-1R and IGF-2R in porcine preimplantation embryos. Biol. Reprod. 54: (Suppl 1)74.
47. Machàty, Z., L. Kovacs and R.S. Prather. 1996. Activation of the porcine egg through an exogenous G protein-coupled receptor. Biol. Reprod. 54:(Suppl 1)168.
48. Machàty, Z., L.F Rickords, W.J. Jolliff, R.S. Prather.1996. Receptors involved in calcium signaling in porcine eggs. Dev. Biol. 175:382.
49. Machàty, Z., M.A. Mayes, R.S. Prather. 1996. Inositol 1,4,5-triphosphate induces parthenogenesis in porcine oocytes. Theriogenology 45:155.
50. Li, J., R.S. Prather, C.L. Markert. 1996. Decondensation of hamster chromosomes in the pronuclei of 1-cell stage mouse embryos following chromosome microinjection. Theriogenology 45:346.
51. Prather, R.S. 1997. Cloning Mammals: Theory, Practice & Potential. Cloning and Biotechnology: Opportunities, Challenges and Choices. November 13, 1997. University of Nebraska-Lincoln.
52. Whitworth, K. M., R.S. Prather. 1997. Cumulative effect of myosin light chain kinase, protein kinase A and protein kinase C inhibition on porcine oocyte activation and appearance of the 22 kDa protein. Biol. Reprod. 56: (Suppl 1)210.
53. Abeydeera, L.R., W. Wang, R.S. Prather, B.N. Day. 1997. In vitro penetration and subsequent development of pig oocytes matured in serum-free culture media. Biol. Reprod. 56: (Suppl 1)215.
54. Machàty, Z., B.N. Day, R.S. Prather. 1997. Thimerosal induces full activation of pig oocytes. Biol. Reprod. 56: (Suppl 1)233.
55. Prather, R.S. 1997. Nuclear and cytoplasmic modifications after nuclear transfer. Mammalian Cloning: Implications for Science and Society, Crystal Gateway Marriott Hotel, Arlington, VA. June 26-27.
56. Wang, W.H., L.R. Abeydeera, T.C. Cantley, A. Rieke, R.S. Prather, B.N. Day. 1997. Morphological compairson of pig oocytes matured in vivo and in vitro. 5th International Conference on Pig Reproduction, Kerkrade, The Netherlands, June 2-4. p. 141.
57. Machàty, Z., B.N. Day, R.S. Prather. 1997. Cell numbers of pig embryos developed in vitro versus in vivo. 5th International Conference on Pig Reproduction, Kerkrade, The Netherlands, June 24. p. 145.
58. Kim, J.-H., Z. Machàty, R. Cabot, R.S. Prather. 1997. Pig oocyte activation by stimulation of an exogenous G protein coupled receptor. 5th International Conference on Pig Reproduction, Kerkrade, The Netherlands, June 2-4. p. 143.
59. Whitworth, K.M., R.S. Prather. 1997. Parthenogenetic activation of pig oocytes by myosin light chain kinase inhibition. Midwest Animal Science Meetings p 86.
60. Abeydeera, L.R., W.H. Wang, T.C. Cantley, R.S. Prather, B.N. Day. 1998. Glutathione content and embryo development after in vitro fertilization of pig oocytes matured with various concentrations of cysteine and a thiol compound. Biol. Reprod. 58: (suppl 1)92.
61. Anderson, J.E., R.L. Matteri, R.S. Prather. 1998. Cyclin B1 transcript levels during the transition from maternal to zygotic control of development in porcine embryos. Biol. Reprod. 58: (Suppl 1)72.
62. Ruddock, N., W.H. Wang, Z. Machàty, B.N. Day, R.S. Prather. 1998. Intracellular pH changes accompany parthenogenic activation in the porcine oocyte. Biol. Reprod. 58: (Suppl 1)275.
63. Han, Y.M., L.R. Abeydeera, J.H. Kim, A. Petersen, B.N. Day, R.S. Prather.1998. In vivo development and ploidy of poly-pronuclear pig eggs. Biol. Reprod. 58: (Suppl 1)394.
64. Wang, W.H., L.R. Abeydeera, Y.M. Han, R.S. Prather, B.N. Day. 1998. Morphological evaluation and actin cytoskeleton distribution of porcine embryos produced in vitro and in vivo. Biol. Reprod. 58: (Suppl 1)74.
65. Machàty, Z., R.S. Prather. 1998. Capacitative calcium entry in porcine oocytes. Biol. Reprod. 58: (Suppl 1)274.
66. Funahashi, H., Z. Machaty, W.H. Wang, TC Cantley, R.S. Prather, B.N. Day. 1998. Intracellular calcium release and cortical reaction after thimerosal-treatment of porcine oocytes matured in vitro or ovulated. Biol. Reprod. 58(Suppl 1):472.
67. Vajta, G, C. Murphy, R.S. Prather, Z. Machàty, T. Greve, H. Callesen. 1998. In-straw dilution of in vitro produced bovine blastocysts after vitrification with the open pulled straw (OPS) method. International Congress on Animal Reproduction, Milan, Italy.
68. Wang, H.W., Z. Machàty, N. Ruddock, L.R. Abeydeera, A. Boquest, R.S. Prather, B.N. Day. 1998. Activation of pig oocytes with calcium ionophore in calcium-free medium. Midwestern Animal Science Meetings P74 #180.
69. Abeydeera, L.R., W.H. Wang, R.S. Prather, B.N. Day 1998. Effect of the source of different hormonal supplements on nuclear maturation, fertilization and development in vitro of pig oocytes matured in a protein-free medium. Midwestern Animal Science Meetings.
70. Overström, E.W., A. Baguisi, M. Mayes, R.S. Prather. 1998. Differential expression of E-cadherin and Na+/K+ ATPase proteins in parthenogenetic pig embryos. Theriogenology 49:189.
71. Kim, J.H., Z. Machàty, R. Cabot, Y.M. Han, R.S. Prather. 1998. Pig oocyte activation by stimulation of an exogenous G protein coupled receptor. Theriogenology 49:154.
72. Machàty, Z., R.S. Prather. 1998. Characteristics of thimerosal-induced activation of porcine oocytes. Theriogenology 49:156.
73. Han, Y.M., L.R. Abeydeera, A. Petersen, J.H. Kim, C.N. Murphy, R.S. Prather. 1998. Preimplantation development of polyspermic pig eggs derived from in vitro maturation and fertilization. Theriogenology 49:284.
74. Ruddock, N.T., Z. Macháty, W.H. Wang, R.S. Prather. 1999. Species-specific differences in intracellular pH changes during parthenogenetic activation. Theriogenology 51:364.
75. Han, Y.M., W.H. Wang, L.R. Abeydeera, J.H. Kim, B.N. Day, R.S. Prather. 1999. How do poly-spermic pig eggs develop in vitro and in vivo? Theriogenology 51:184.
76. Abeydeera, L.R., W.H. Wang, T.C. Cantley, A. Rieke, R.S. Prather, B.N. Day. 1999. Epidermal growth factor can enhance the developmental competence after in vitro fertilization of pig oocytes matured under protein-free culture conditions. Theriogenology 51:365.
77. Perry, G. A., R.S. Prather, M.F. Smith. 1999. In vitro meiotic competence of oocytes collected from porcine antral follicles. MidWest Section of the American Society of Animal Scientists. 77 (Suppl 1):73.
78. Abeydeera, L.R., W.H. Wang, R.S. Prather, B.N. Day. 1999. Effect of incubation temperature on in vitro maturation of pig oocytes: subsequent fertilization and development. MidWest Section of the American Society of Animal Scientists. 77 (suppl 1):74
79. Tao, T., Z. Macháty, A. Boquest, B. Kűhholzer, B. Day, R. Prather. 1999. Production of pig embryos by intracytoplasmic injection of cultured fetal fibroblast cells. Biol. Reprod. 60 Suppl 1:268.
80. Perry, G.A., R.S. Prather, K.D. Alsup, L.M. Falch, M.F. Smith. 1999. Protein synthesis and secretion by preantral, early antral, and antral porcine follicles. Biol. Reprod. 60 Suppl 1:110.
81. Macháty, Z., N.T. Ruddock, R.S. Prather. 1999. Sodium/calcium exchange in porcine oocytes. Biol. Reprod. 60 Suppl 1:105.
82. Cabot, R.A., M.H. Hannink, R.S. Prather. 1999. Leptomycin B arrests bovine and porcine embryo development at the major turn-on of the embryonic genome. Biol. Reprod. 60 Suppl 1:192.
83. Kim, J.H., H.J Do, W.H. Wang, Y.M. Han, B.N. Day, R.S. Prather. 1999. Induction of pig oocyte activation via an increase in tyrosine kinase activity. Biol. Reprod. 60 Suppl 1:107.
84. Do, H.J., J.H. Kim, L.R. Abeydeera, Y.M. Han, J. Green, R.M. Roberts, R.L. Matteri, B.N. Day, R.S. Prather. 1999. Expression of pregnancy-associated glycoprotein 1 and 2 genes in in vitro and parthenogenetically derived pig blastocysts. Biol. Reprod. 60 Suppl 1:250.
85. Ruddock, N.T., Z. Macháty, R.S. Prather. 1999. Intracellular pH changes during parthenogenetic activation of porcine oocytes are sodium-independent when using 200 uM thimerosal or calcium ionophore, and sodium-dependent when using 7% ethanol. Biol. Reprod. 60 Suppl 1:105.
86. Abeydeera, L.R., R.S. Prather, B.N. Day. 1999. Developmental ability of in vitro derived pig embryos in four different culture media. Biol. Reprod. 60 Suppl 1:128.
87. Anderson, J.E., R.L. Matteri, L.R. Abeydeera, B.N. Day, R.S. Prather. 1999. Are cyclin B1 transcripts produced by the in vitro derived 4-cell stage porcine embryo? Biol Reprod. 60 Suppl 1:129.
88. Day, B.N., L.R. Abeydeera, R.S. Prather. 1999. Recent progress in pig embryo production through in vitro maturation and fertilization techniques. IV International Congress on Boar Sperm Preservation, Aug 8-11, Beltsville, MD.
89. Kűhholzer, B., Z. Macháty, T.Tao, L. Lai, B.N. Day, R.S. Prather. 2000. Porcine nuclear transfer embryos derived from fetal fibroblasts synchronized in the G0 versus the G1-stage. Theriogenology 53:229.
90. Macháty, Z., L.R. Abeydeera, J. Thompson, B.N. Day, R.S. Prather. 2000. Inhibition of oxidative phosphorylation and its effect on porcine embryonic development. Theriogenology 53:277.
91. Lai, L., T. Tao, Z. Macháty, B. Kűhholzer, B.N. Day, R.S. Prather. 2000. Feasibility of production of nuclear transfer pigs by using G2/M stage fibroblasts as donors. Theriogenology 53:231.
92. Ruddock, N.T., R.S. Prather. 2000. Intracellular pH changes during parthenogenetic activation of porcine oocytes: bicarbonate dependence, and H2DIDS and bafilomycin A1 sensitivity. Theriogenology 53:441.
93. Tuggle, C.K., K. Whitworth, J.A. Green, R. Prather, M.F. Rothschild, D. Pomp, L. Messer, C. Roberts, T. Casavant, M.B. Soares. 2000. Gene discovery in porcine reproduction: physical mapping of 19 genes and ESTs. Plant and Animal Genome VIII .p 201.
94. Prather, R.S. 2000. Development of Embryo-Technologies in Swine. Missouri Veterinary Medical Association.
95. Prather, R.S., N.T. Ruddock, Z. Macháty. 2000. Parthenogenetic activation of swine oocytes. International Congress on Animal Reproduction. W11:2.
96. Macháty, Z., A.J. Bonk, B. Kűhholzer, R.S. Prather. 2000. Microinjection of a crude sperm extract activates porcine oocytes. International Congress on Animal Reproduction. 18:10.
97. Kűhholzer, B., M. Schoenfeld, R.S. Prather. 2000. Reversible synchronization of porcine fetal fibroblast cells with a topoisomerase II inhibitor. Biol. Reprod. Suppl 62 1:317.
98. Lai, L., B. Kühholzer, T. Tao, K.-W. Park, B.N. Day, R.S. Prather. 2000. Porcine nuclear transfer embryos derived from injection of G2/M stage fibroblasts into preactivated in vitro matured oocytes. Biol. Reprod. Suppl 62 1:192.
99. Wei, Z.-X., J.A. Green, R.M. Roberts, R.S. Prather. 2000. Identification of a novel Kunitz-type proteinase inhibitor expressed by early porcine embryos. Biol. Reprod. Suppl 62 1:312.
100. Park, K.W., B. Kühholzer, L. Lai, Z. Macháty, B.N. Day, R.S. Prather. 2000. Development of porcine nuclear transfer embryos derived from granulosa cells. Biol. Reprod. Suppl 62 1:321.
101. Ruddock, N.T., R.S. Prather. 2000. Parthenogenetic activation of porcine oocytes: importance of calcium and pH. Biol. Reprod. Suppl 62 1:216.
102. Prather, R.S., B. Kühholzer, L. Lai, K.W. Park. 2000. Changes in the structure of nuclei after transfer to oocytes. Genetically Engineering and Cloning Animals, Park City, Utah. June 18-20.
103. Prather, R.S., L.R. Abeydeera, B.N. Day, M.L. Leibfried-Rutledge. 2000. A preliminary study on comparison of two different in vitro maturation systems for pig oocytes on subsequent embryo development. Cloning 2:159.
104. Shi, X.W., C.J. Fitzsimmons, R. Prather, K. Whitworth, J.A. Green, C.K. Tuggle. 2000. High-resolution physical mapping confirms similar gene order with an inversion between human 17 (Hsap17) and porcine chromosome 12 (Sscr12). International Conference on Animal Genetics, Minneapolis, MN July 22-26.
105. Tuggle, C.K., C.J. Fitzsimmons, R. Woods, K. Whitworth, J.A. Green, R. Prather, C. A. Roberts, T. Casavant, M. B. Soares. 2000. Development of new placental and fetal ESTs for gene discovery in pig reproduction. International Conference on Animal Genetics. Minneapolis, MN July 22-26. #A065
106. Van Vickle, S.J., R.A. Cabot, R.S. Prather. 2000. Cloning and sequencing of five importin alpha homologues in pigs. 11th Undergraduate Science Research at MU, August 3.
107. Critser, J.K., R.S. Prather. 2000. The application and relevance of nuclear transfer technology to conservation. London Symposium on Conservation.
108. Kűhholzer, B., R.J. Hawley, L. Lai, R.S. Prather. 2001. Nuclear transfer using different sub-clones of porcine fetal fibroblast cells result in different in vitro development. Therio. 55:276.
109. Park, K.-W., B. Kűhholzer, L. Lai, B.N. Day, R.S. Prather. 2001. Development of porcine nuclear transfer embryos derived from mural granulosa cells. Therio. 55:282.
110. Lai, L., K.-W. Park, B. Kűhholzer, Q. Sun, R.A. Cabot, R.S. Prather. 2001. Early in vitro development of fertilized nuclear transfer pig oocytes. IETS Therio. 55:277.
111. Cabot, R.A., M. Hannink, R.S. Prather. 2001. Nuclear trafficking in porcine oocytes. Theriogenology 55:466.
112. Tuggle, C.K., J. Green, C. Fitzsimmons, R. Woods, R.S. Prather, S. Malchenko, M.B. Soares, C.A. Roberts, K. Pedretti, T. Casavant, C. Harger, Y. Zhang, M.F. Rothschild. 2001. Update on development of resources for functional genomics in the pig. Plant and Animal Genome IX. W208.
113. Tuggle, C.K., J.A. Green, C. Fitzsimmons, R. Woods, R.S. Prather, S. Malchenko, M.B. Soares, C.A. Roberts, K Pedretti, T. Casavant, C. Harger, Y. Zhang, M.F. Rothschild. 2001. Development of resources for functional genomics in the pig: production of 14 cDNA libraries and sequencing of over 7,000 clones from female reproductive tissues. Plant and Animal Genome IX. P67.
114. Cheong, H.T., K.W. Park, G.S. Im, L. Lai, Q.Y. Sun, B.N. Day, R.S. Prather. 2001. Effect of elevated Calcium concentration in fusion medium on the fusion and development of porcine fetal fibroblast cell nuclear transfer. 6th International Conference on Pig Reproduction, June 3-6, Columbia, MO, USA. P 144.
115. Macháty, M., J. J. Ramsoondar, A.J. Bonk, K.R. Bondioli, R.S. Prather. 2001. The presence of trp in porcine oocytes. 6th International Conference on Pig Reproduction, June 3-6, Columbia, MO, USA. P 45.
116. Mao, J., M.F. Smith, R.S. Prather, B.A. Didion, R.S. Prather. 2001. Effect of follicular isolation method on porcine perantral follicular development, in vitro. 6th International Conference on Pig Reproduction, June 3-6, Columbia, MO, USA. p 49.
117. Park, K.W., L. Lai, H.T. Cheong, G.S. Im, Q.Y. Sun, B.N. Day, R.S. Prather. 2001. Optimization of cell fusion/activation methods in nuclear transfer by using transgenic porcine cells. 6th International Conference on Pig Reproduction, June 3-6, Columbia, MO, USA. P 142.
118. Wu, G, Q. Sun, J. Mao, L. Lai, T.C. McCauley, S. Lu, K.-W. Park, R.S. Prather, B.A. Didion, B.N. Day. 2001. Reversible inhibition of in vitro maturation of porcine oocytes by a specific MPF kinase inhibitor, butyrolactone I. 6th International Conference on Pig Reproduction, June 3-6, Columbia, MO, USA. P 48.
119. Wu, G, Q. Sun, J. Mao, L. Lai, T.C. McCauley, S. Lu, K.-W. Park, R.S. Prather, B.A. Didion, B.N. Day. 2001. The effect of various calcium/magnesium ratios in the embryo culture medium on the development of pig embryos. 6th International Conference on Pig Reproduction, June 3-6, Columbia, MO, USA. P 73.
120. Mao, J., G. Wu, M.F. Smith, R.S. Prather, B.A. Didion, B.N. Day. 2001. Effects of media and serum type on porcine preantral follicular development in vitro. Biol. Reprod. 64 Suppl 1: 154-155.
121. Park, K.W., L. Lai, H.T. Cheong, G.S. Im, Q.Y. Sun, G. Wu, B.N. Day, R.S. Prather. 2001. Cytochalasin B is not necessary during cell injection for nuclear transfer when using transgenic porcine cells. Biol. Reprod. 64 Suppl 1:124.
122. Prather, R.S. 2001. Preserving/conserving germplasm by incorporating embryo-related technologies. IAAFSC, abstract 51.
123. Tuggle, C.K., J.A.Green, C. Fitzsimmons, R.Woods, R. Prather, S. Malchenko, M. B. Soares, C.A. Roberts, K. Pedretti, T. Casavant, D. Pomp, Yuandan Zhang, M. F. Rothschild, C. Harger, W. Beavis. 2001. Production of 18 cDNA libraries and successful EST sequencing of 10,124 clones from porcine female reproductive tissues. IAAFSC.
124. D.B. Carter, R.S. Prather, C.F. Franklin. 2001. Specialized periparturient care in a genetically engineered swine colony. Presented at the 52nd annual AALAS National Meeting, Baltimore MD. October 21-25, 2001.
125. Lai, L., Q.-Y. Sun. K.W. Park, R.S. Prather. 2002. Artificial activation of in vitro matured oocytes before fertilization can reduce the polyspermy rate. Theriogenology 57:674.
126. Viuff, D., L.F. Rickords, L. Lai, H.-T. Cheong, M. Samuel, R.S. Prather, T. Greve, P.D. Thomsen. 2002. The activation of ribosomal RNA genes in nuclear transfer porcine embryos. Theriogenology 57:455.
127. Park, K.-W., L. Lai, H.-T. Cheong, R. Cabot, Q.-Y. Sun, G. Wu, B.N. Day, R.S. Prather. 2002. Developmental ability of nuclear transfer embryos by using ear skin fibroblasts derived from a transgenic pig. Theriogenology 57:442.
128. Cabot, R.A., M. Hannink, R.S. Prather. 2002. Nuclear export in the porcine oocyte. Theriogenology 57:491.
129. Tuggle, C.K., J.A. Green, C. Fitzsimmons, R. Woods, R.S. Prather, S. Malchenko, M.B. Soares, C.A. Roberts, K. Pedretti, T. Cassavant, D. Pomp, G. Bertani, S. Olberding, Q. Zhang, M.F. Rothschild, K. Garwood, W. Beavis. 2002. Pig reproduction EST project: Production of 20 cDNA libraries, 14,602 sequences and 49 map locations for genes expressed in major female reproductive tissues. Plant and Animal Genome, p 82.
130. Park, K.-W., L. Lai, G. Wu, B.N. Day, R.S. Prather. 2002. Effects of FCS on in vitro development of porcine nuclear transfer embryos. Biol. Reprod. Suppl. 1, p 234.
131. Wu, G.-M., J. Mao, L. Lai, T.C. McCauley, K.-W. Park, A.R. Rieke, T.C. Cantley, M.F. Smith, R.S. Prather, B.A. Didion, B.N. Day. 2002. Magnesium inhibits in vitro fertilization of pig oocytes. Biol. Reprod. Suppl. 1, p 311.
132. Mao, J., M.F. Smith, E. Rucker, G.-M. Wu, R.S. Prather, B.A. Didion, B.N. Day. 2002. Insulin-like growth factor-1 suppressed apoptosis of granulosa cells and stimulated perantral follicular growth in vitro. Biol. Reprod. Suppl. 1, p 212.
133. T. McCauley, K. van Leyen, L. Lai, M. Sutovsky, B.N. Day, R.S. Prather, R. Oko, P. Sutovsky.2002. Unique aspects of zygotic development in pig after in vitro fertilization and cloning. Biol. Reprod. Suppl. 1, p 311.
134. Whitworth, K.W., M.C. Lucy, J. Ries, G. Springer, A. Guillen, J.L. Forrester, N. Mathialagan, B.A. Didion, R.S. Prather, J.A. Green. 2002. Characterization of cDNA libraries developed from porcine oocytes and embryos: Identification of differentially expressed genes. Biol. Reprod. Suppl. 1, p 159.
135. Green, J.A., K.W. Whitworth, J. Ries, G. Springer, A. Guillen, J.L. Forrester, N. Mathialagan, B.A. Didion, M.C. Lucy, R.S. Prather. 2002. Characterization of cDNA libraries developed from porcine uterus and oviduct: Identification of differentially expressed genes. Biol. Reprod. Suppl. 1, p 319.
136. Cabot, R.A., R.S. Prather. 2002. Injection of double stranded mRNA molecules corresponding to the importin alpha homologue HSRP1 reduced developmental potential of porcine embryos. Biol. Reprod. Suppl. 1, p 148.
137. Sutovsky, P., A. Wu, K.W. Park, R.S. Prather, D.B. Carter, D. Takahashi, T. Dominko, M. Sutovsky, R. Oko. 2002. PT32: Sperm perinuclear theca protein with properties of the sperm-borne, oocyte-activating factor. Biol. Reprod. Suppl. 1, p 275.
138. Prather, R.S., G. Springer, L.J. Forrester, K.M. Whitworth, J. Ries, N. Bivens, A. Guillen, N. Mathialagan, B.A. Didion, J.A. Green. 2002. Construction of porcine specific gene arrays and their use for characterizing gene expression during early development. First International Meeting on Mammalian Embryo Genomics, July 20, Quebec City, Canada. p 15.
139. Jiang, H., K.M. Whitworth, N. Bivens, J. Ries, J.A. Green, L.J. Forrester, G.K. Springer, B.A. Didion, N. Mathialagan, R.S. Prather, M.C. Lucy. 2002. Large-scale generation and analysis of expressed sequence tags from porcine ovaries or ovarian follicles at different stages of development. ASAS/ADSA J. Anim. Sci. 80:suppl 1, p 301-302.
140. Spollen, W.G., G.K. Springer, R.S. Prather. 2002. Cytoplasmic polyadenylation elements in swine reproductive tissues. American Medical Informatics Association.
141. Sutovsky, P., T. McCauley, W. Thompson, K.A. Fisher, B.N. Day, R.S. Prather, T. Safranski, M. Sutovsky. 2002. Ubiquitin in mammalian spermatogenesis, fertilization, and preimplantation embryonic development: killing three birds with one stone.
142. Tuggle, C.K., J.A. Green, C. Fitzsimmons, R. Woods, R.S. Prather, S. Malchenko, M.B. Soares, C.A. Roberts, K. Pedretti, T. Casavant, D. Pomp, A.R. Caetano, J.B. Edeal, S. Olberding, Y.D. Zhang, M.F. Rothschild, K. Garwood, W. Beavis. 2002. Improving the human-pig comparative map: Sequencing of 16,000 ESTs and Mapping 100-pig genes with human orthologs. International Society on Animal Genetics.
143. Isom, S.C., R.S. Prather, E.B. Rucker. 2003. Heat-induced apoptosis in preimplantation porcine embryos. Theriogenology 59:323.
144. Caamano, J.N., G.M. Wu, T.C. McCauley, A.R. Rieke, T.C. Cantley, J. Mao, B.A. Didion, R.S. Prather, B.N. Day. 2003. Non-surgical embryo transfer in swine: preliminary results. Theriogenology 59:361.
145. Mao, J., M.F. Smith, B. Didion, G. Wu, T.C. McCauley, T.C. Cantley, R.S. Prather, M. McDaniel, B.N. Day. 2003. IGF-I and EGF regulated preantral follicular growth and development. Theriogenology 59:410.
146. Pedersen, H.G., Z. Rasmussen, D. Viuff, P.D. Thomsen, L. Lai, L. Rickords, R.S. Prather, T. Greve. 2003. Chromosome abnormalities in in vitro developed, in vitro produced or parthenogenetically activated porcine embryos. Theriogenology 59:430.
147. Mao, J., N. Caamano, T. Cantley, R. Farwell, A. Rieke, M.F. Smith, R. Prather, B.N. Day. 2003. Effect of follicular size on developmental competence of porcine oocytes in vitro. J. Anim. Sci. 81: suppl. 1 p. 187.
148. Tuggle, C.K., J.A. Green, C. Fitzsimmons, R. Woods, R.S. Prather, S. Malchenko, M.B. Soares, T. Kucaba, K. Crouch, C. Smith, D. Tack, N. Robinson, B. O’Leary, T. Sheetz, T. Casavant, D. Pomp, J.B. Edeal, Y. Zhang, Z. Hu, M.F. Rothschild, K. Garwood, W. Beavis. 2003. Completion of the Midwest consortium project: sequencing of 21,499 reproduction ESTs and comparative mapping of 721 selected genes. J. Anim. Sci. 81: suppl. 1 p. 2.
149. Im, G.-S., L. Lai, Y. Hao, Z. Liu, D. Wax, A. Bonk, R.S. Prather. 2003. In vitro development of porcine nuclear transfer embryos cultured in different media and gas atmospheres. SSR Biol. Reprod. 68 Suppl. 1. p 339.
150. Hao, Y., L. Lai, J. Mao, G. Im, A. Bonk, R.S. Prather. 2003. Apoptosis of porcine embryos derived from parthenogenetic activation during preimplantation development in vitro. SSR Biol. Reprod. 68 Suppl. 1. p 338.
151. Lai, L., Z. Liu, G.-S. Im, Y. Hao, R.S. Prather, 2003. In vitro development of nuclear transfer embryos constructed by using fibroblasts derived from parthenogenetic fetuses as donors. SSR. Biol. Reprod. 68 Suppl. 1. p 337.
152. Ruddock, N.T., K. Wilson, R.S. Prather, A.J. French. 2003. Expression of the six known isoforms of the Na+/H+ exchange (NHE) genes in cDNA pools produced from single bovine oocytes and IVP blastocysts. SSR. Biol. Reprod. 68 Suppl. 1. p160.
153. Schatten, H., R.S. Prather, H.-Y. Fan, C. Tong and Q.-Y. Sun. 2003 Regulation of cytoskeletal functions in pig oocytes. Microscopy and Microanalysis, Volume 9 (Suppl 2), 2003, 1200-1201
154. Prather, R.S. 2003. Transgenic pigs for medicine and agriculture. Transgenic Animal Research Conference III, Lake Tahoe, CA. Aug . 11-14.
155. Yamada, K, K. Yazawa, C Kamnon, A. Shimizu, S. Moran, M. Nuhn, K. Teranishi, P. Vagefi, R. Prather, E.J. Forsberg, R. Hawley, H.J. Schuurman, M. Awwad, J. Greenstein, J. Fishman, D.K.C. Cooper, M. Sykes, D.H. Sachs, K. Yamada. 2003. An initial report of alpha-Gal deficient pig to baboon renal xenotransplantation: evidence for the benefit of co-transplantaing vascularized donor thymic tissue. Xenotransplantation 10(5): 480.
156. Tseng, Y.-L., F.J.M.F. Dor, K. Kuwaki, D. Prabharasuth, A. Alt, D.Ryan, M. Denaro, M. Giovino, J. Wood, R. Prather, E.J. Forsberg, K. Yamada, R. Hawley, M. Awwad, C. Patience, H.J. Schuurman, D.H. Sachs, D.K.C. Cooper. 2003. Initial results of xenotransplantation (Tx) in baboons using hearts from 1,3-galactosyltransferae gene-knockout (GT-KO) pigs. Xenotransplantation 10(5):486.
157. Beschorner, W., S.S. Joshi, R. Prather, T. Schieber, S.C. Thompson, T. Yang T, L. Zhou, S. Mirvish. 2003 Selective and conditional depletion of pig cells with transgenic pigs and specific liposomes. Xenotransplantation 10 (5): 497.
158. Kuwaki, K., Y.L. Tseng, F.J.M.F. Dor, S. Houser, D. Prabharasuth, A. Alt, R. Hawley, R. Prather, E.J. Forsberg, A. Shimizu, K. Yamada, D.H. Sachs, H.J. Schuurman, M. Awwad, D.K.C. Cooper. 2003. Initial results of xenotransplantation (Tx) in baboons using hearts from 1,3-galactosyltransferase gene-knockout (GT-KO) pigs. Xenotransplantation 10 (5): 526.
159. Beschorner, W., R. Prather, C. Sosa, S.C. Thompson, T. Schieber, T. Yang. 2003. Transgenic pigs expressing the suicide gene thymidine kinase in the liver. Xenotransplantation 10 (5): 530
160. Vajta, G., T.T. Peura, L. Lai, C. Murphy, R.S. Prather, P.M. Kragh, H. Callesen. 2004. Highly efficient and reliable chemically assisted enucleation method for hand made cloning (HMC). IETS Reproduction Fertility & Development 16:159.
161. Im, G.-S., L. Lai, Z. Liu, Y. Hao, C.N. Murphy, R.S. Prather. 2004. Improved in vitro development of porcine nuclear transfer embryos with 6-DMAP following fusion. Reproduction Fertility & Development 16:144.
162. Liu, Z.H., L.X Lai, G.S Im, M. Samuel, D. Wax, R.S. Prather. 2004. Effect of pig follicle fluid and fetal calf serum on porcine oocyte maturation and subsequent development after activation and somatic cell nuclear transfer. Reproduction Fertility & Development 16:278.
163. Agca, C., J.E. Ries, S. Kolath, J.-H. Kim, G.K. Springer, L.J. Forrester, E. Antoniou, K. Whitworth, N. Mathialagan, R.S. Prather, M.C. Lucy. 2004. Ovarian follicle gene expression before and after the preovulatory lutenizing hormone (LH) surge in swine (sus scorfa domestica). Plant and Animal Genome page 279 (abstract p834).
164. Shatos, M.A., H. Klassen, P.H. Schwartz, J. Doherty, B. Ziaeian, I. Kirov, R.S. Prather, M.J. Young. 2004. Isolation of cells from retina and brain of the GFP-transgenic pig. Invest. Ophth. & Vis. Sci. 25: U647 5406 Suppl. 2. www.arvo.org.
165. Klassen, H., K. Warfvinge, J.F. Kiilgaard, E. Scherfig, J. Prause, M.A. Shatos, R.S. Prather, M.J. Young. 2004. Transplantation of retinal progenitor cells from GFP-transgenic pigs to the injured retina of allogeneic recipients. Invest. Ophth. & Vis. Sci. 25: U646 5400 Suppl. 2. www.arvo.org.
166. Patel, R.V., C. Agca, R. Prather, G.K. Springer. 2004. Autoannotation program to determine annotation of clones used in microarray experiments. Univ. MO Life Sciences Week.
167. Sutovsky P., L. Lai, G. Manandhar, M. Sutovsky, J. Caamaño, B.N. Day and R.S. Prather. 2004. Proteasomal activity is required for pronuclear development after IVF, and for donor cell-nuclear remodeling after SCNT. SSR Biol. Reprod. Special Issue p 225-226.
168. Lai, L, G.S. Im, Z. Liu, Y. Hao, A. Rieke, M. Samuel, D. Wax, C.N. Murphy, R.S. Prather. 2004. Comparison of in vivo developmental competency of cloned pig embryos derived from three different activation strategies. SSR Biol. Reprod. Special Issue p 212.
169. Liu, Z.H., Y.H Hao, L.X Lai, D. Wax, M. Samuel, H. Schatten, R.S Prather. 2004. Morphology and dynamics of alpha-tubulin and nuclear mitotic apparatus protein during the first cell cycle in porcine nuclear transfer embryos, parthenogenetic embryos and in vitro fertilization embryos. SSR Biol. Reprod. Special Issue p 215.
170. Hao, Y.H., L.X. Lai, Z.H. Liu, G.S. Im, D. Wax, M. Samuel, R.S Prather. 2004. Chromosomal abnormalities and developmental competence in porcine electrically-activated parthenogenetic blastocysts. SSR Biol. Reprod. Special Issue p 214.
171. Prather, R.S. 2004. Genetic modification of pigs for medicine and agriculture. SSR. Biol. Reprod. Special Issue p 81.
172. Agca, C., K.M. Whitworth, J.-G. Kim, C.N. Murphy, A. Rieke, G.K. Springer, L.J. Forrester, J.A. Green, N. Mathialagan, R.S. Prather, M.C. Lucy. 2004. Microarray analysis of gene expression during ovarian development in swine. J. Anim. Sci. 82(suppl 1) 200.
173. Prather, R.S. 2004. Swine as biomedical models. Workshop on Advantages of Domestic Species as Biomedical Models. Michigan State University. October 29-31.
174. Im, G.S., B.S.Yang, L. Lai, Z. Liu, Y. Hao, R.S. Prather. 2005. High osmolarity during early culture stage improves in vitro development of pre-implantation porcine nuclear transfer embryos. International Embryo Transfer Society annual meeting. Reprod. Fert. & Dev. P 169.
175. Overman, L.M., C. Besch-Williford, L. Lai, H.-T. Cheong, G.-S. Im, K.-W. Park, C. Murphy, J.A. Green and R.S. Prather. 2005. Histological Comparisons between Nuclear Transfer and In Vivo Porcine Embryos. International Embryo Transfer Society annual meeting. Reprod. Fert. & Dev. P 180.
176. Caamaño, J.N, M. Hermsen, J. Marcos, A. Meana, F. Goyache, M. Prieto, A. Espí, L.J. Royo, C. Diez, G. Pajares, S. Borragán, R.S.Prather, E. Goméz. 2005. A procedure to obtain fibroblasts from wild animals. International Embryo Transfer Society annual meeting. Reprod. Fert. & Dev. P 245.
177. Kim, J.G., K.M. Whitworth, C. Agca, G.K. Springer, R. Patel, N.J. Bivens, L.J. Forrester, R.S. Prather, J.A. Green. 2005. Comparative transcriptional profiling of porcine emdometrium during the estrous cycle by using cDNA microarray. Plant & Animal Genome P702.
178. Whitworth, K.M., C. Agca, J.G. Kim, G.K. Springer, R. Patel, N. Bivens, L.J. Forrester, J.A. Green, R.S. Prather. 2005. Comparison of differential expression patterns in porcine oocytes and early embryos by using cDNA microarrays. Plant & Animal Genome P722.
179. Bonk, A., M. Samuel, L. Lai, Z. Liu, Y. Hao, E. Antoniou, R.S. Prather. 2005. Microarray based detection of differential CpG methylation in pig kidney and spleen tissues. Plant & Animal Genome P701
180. Riley, L.K., J.K. Critser, R.S. Prather, T. Strauch. 2005. The National Swine Resource and Research Center. Swine in Biomedical Research Conference. Jan 27-29, Chicago, Il. Pg 41.
181. Whitworth, K.M., C. Agca, J.G. Kim, R. Patel, G.K. Springer, N. Bivens, L.J. Forrester, N. Mathialagan, J.A. Green, R.S. Prather. 2005. Transcriptional profiling of in vitro versus in vivo produced pig embryos by using a 15K member unigene set specific for pig reproductive tissues and embryos. Swine in Biomedical Research Conference. Jan 27-29, Chicago, Il. Pg 38.
182. Carroll, J.A., D.B. Carter, S.W. Korte, S.E. Dowd, R.S. Prather. 2005. The acute phase response of cloned pigs following an immune challenge. Southern Section of the American Society of Animal Science.
183. Lai, L., Z. Liu, Y. Hao, D. Wax, R.S. Prather. 2005. In vitro Development of Fertilized Nuclear Transfer Embryos Derived from Swine Parthenogenetic Cells. International Conference on Pig Reproduction p 159.
184. Riley, L.K., J.K. Critser, R.S. Prather, T. Strauch. 2005. The National Swine Resource and Research Center. International Conference on Pig Reproduction p 163.
185. Ross, J.W., M.D. Ashworth, D.R. Stein, U. DeSilva, P. Ayoubi, R.S. Prather, K. Whitworth, C. Agca, J.-G. Kim, J.A. Green, F. White, G.A. Johnson, R.D. Geisert. 2005. Identification and analysis of molecular markers following endocrine disruption of pregnancy in the pig. International Conference on Pig Reproduction p 123.
186. Green, J.A., J.-G. Kim, K.M. Whitworth, C. Agca, R.S. Prather. 2005. The use of microarrays to define functionally-related genes that are differentially expressed in the cycling pig uterus. 7th International Conference on Pig Reproduction p 59.
187. Isom, S.C., C. Alinea, J. Dillender, R.S. Prather, E.B. Rucker, III. 2005. Differential response of porcine preimplantation embryos to timing variations of an exogenously applied heat stress. SSR meeting. Biology of Reproduction Special issue p 153.
188. Lai, L, S.C. Isom, R. Li, Y. Hao, D. Wax, E.B. Rucker, III, R.S. Prather. 2005. In vitro development of porcine nuclear transfer embryos with treatment of different initial culture temperatures after activation. SSR meeting. Biology of Reproduction Special issue p 144.
189. Hao, Y.H., H.Y. Yong, C.N. Murphy, D. Wax, M. Samuel, A. Rieke, E.M. Price, R.M. McAllister, J.R. Turk, E.B. Rucker, III, M.H. Laughlin, R.S. Prather. 2006. Production of transgenic clones piglets by using porcine fetal fibroblasts over-expressing endothelial nitric oxide synthase (eNOS). IETS. Reproduction, Fertility and Development Abs #374, p 294.
190. Li, R., L. Lai, D. Wax, Y. Hao, Z. Zhong, C.N. Murphy, A. Rieke, R.S. Prather. 2006. Cryopreservation of porcine embryos derived from somatic cell nuclear transfer. IETS. Reproduction, Fertility and Development Abs #101, p 159.
191. Yong, H.Y., C.N. Murphy, A. Rieke, L. Lai, Y. Hao, R. Li, D. Wax, M. Samuel, S. Korte, K. Whitworth, R.S. Prather. 2006. Production of transgenic piglet by new sperm injection technique. IETS. Reproduction, Fertility and Development Abs #381, p 297.
192. Prather, R.S., S. Korte, R. Woods, L. Spate, N. Bivens, L.J. Forrester, R.V. Patel, G.K. Springer, C.N. Murphy, J.A. Green. 2006. Identification of the predominant transcripts in bovine oocytes, in vitro derived blastocysts and in nuclear transfer blastocysts. IETS. Reproduction, Fertility and Development Abs #264, p 239.
193. Bonk, A.J., M. Samuel, L. Lai, Y.H. Hao, R. Li, Z.H. Liu, C. Murphy, E. Antoniou, R.S. Prather. 2006. DNA methylation in porcine gametes, in vitro, in vivo, parthenogenetic and nuclear transfer produced embryos. IETS. Reproduction, Fertility and Development Abs #1, p 109.
194. Korte, S., G.K. Springer, W. Spollen, R.V. Patel, K. Whitworth, N. Bivens,, L.J. Forrester, C.N. Murphy, J.A. Green, R.S. Prather. 2006. Differential transcription in porcine germinal vesicle and metaphase II oocytes. IETS. Reproduction, Fertility and Development Abs #254, p 234
195. Isom, S.C., R.S. Prather, E.B. Rucker, III. 2006. Heat stress enhances parthenogenetic development of porcine embryos, but not in vitro fertilized embryos. IETS, Reproduction, Fertility and Development Abs #164, p 190.
196. Jiang, L., I. Polejaeva, P. Jobst, D. Ayers, R.S. Prather, X. Yang, X.C. Tian. 2006. Expression of growth-related imprinted genes in decreased newborn and surviving cloned piglets. IEST. Reproduction, Fertility and Development Abs #249, p 232.
197. Men, H., Y. Agca, S.F. Mullen, L.K. Riley, R.S. Prather, J.K. Critser. 2006. Porcine embryo quality produced by two embryo culture media as assessed by total cell number and time course of blastocyst hatching. IETS. Reproduction, Fertility and Development Abs #155, p 185.
198. Men, H., Y. Agca, L.K. Riley, R.S. Prather, J.K. Critser. 2006. Forskolin induced increase in lipolytic activity in porcine embryos produced in vitro. IETS . Reproduction, Fertility and Development Abs #169, p 192.
199. Kim, J.G., K.M. Whitworth, R.S. Prather, J.A. Green. 2006. Differential expression of ornithine decarboxylase-1 and neuromedin U in the non-pregnant and pregnant uterine endometrium of swine. Plant and Animal Genome meeting. Abs # P812, p303.
200. Price, E.M., R.S. Prather, M.S. Samuel, M.A. Tanner, S.L. Latcham. 2006. Neurogenic peripheral blood-derived adult stem cells. Experimental Biology annual meeting. 685.2.
201. Price, E.M., R.S. Prather, M.S. Samuel, M.A. Tanner, S.L. Latcham. 2006. Angiogenic peripheral blood-derived adult stem cells. Experimental Biology annual meeting. 412.1.
202. Zhong, Z.-S., M. Katayama, Z.H. Liu, Y.H. Hao, L.X. Lai, D. Wax, M. Samuel, Q.Y. Sun, R.S. Prather, H. Schatten. 2006. Translocation of mitochondria in cloned porcine embryos. Microscopy Society of America.
203. Ross, J.W., M.D. Ashworth, D.R. Stein, U. DeSilva, P. Ayoubi, R.S. Prather, K.M. Whitworth, J.A. Green, F. White, G.A. Johnson, R.D. Geisert. 2006. Alteration of porcine endometrial gene expression associated with conceptus loss following endocrine disruption with exogenous estrogen. SSR Biol. Reprod. Special Issue p108.
204. Lai, L., R. Li, Y. Tan, Y. Hao, Z. Zhong, D. Wax, H. Schatten, R.S. Prather. 2006. In vitro developmental potential of porcine nuclear transfer embryos. SSR Biol. Reprod. Special Issue p109-110.
205. Antelman, J., G. Manandhar, Y.J. Yi, M. Sutovsky, R.S. Prather, P. Sutovsky. 2006. Involvement of mitochondrial transcription factor (Tfam) in porcine gametogenesis and preimplantation embryo development. SSR Biol. Reprod. Special Issue p110.
206. Li, R., K. Whitworth, L. Lai, L. Spate, A. Rieke, Y. Hao, Z. Zhong, M. Katayama, H. Schatten, R.S. Prather. 2006. The concentration and composition of free amino acids and the osmolarities of porcine oviductal and uterine fluid and their effects on the development of porcine parthenogenetic and IVF embryos. SSR Biol. Reprod. Special Issue p110.
207. Hao, Y., N. Mathialagan, E. Walters, J. Mao, L. Lai, D. Becker, J. Critser, R.S. Prather. 2006. Osteopontin present in the porcine oviduct reduces polyspermy during IVF of porcine oocytes. SSR. Biol. Reprod. Special Issue p138.
208. Caamano, J.N., A. Salas, J. Marcos, A. Rodriguez, C. Diez, S. Borragan, R.S. Prather, E. Gomez. 2006. Flow cytometric cell cycle analysis of cultured brown bear fibroblasts. SSR Biol. Reprod. Special Issue p182.
209. Rogers, C.S., Y. Hao, T. Rokhlina, Z. Yan, J.F. Engelhardt, R.S. Prather, M.J. Welsh. 2006. Gene targeting of pig CFTR: progress toward a large animal model of cystic fibrosis. North American Cystic Fibrosis Meeting (November).
210. Blanton, M.W., B.H. Johnstone, J.L. Mund, P.I. Rogers, T.A. Strauch, R.S. Prather, L.K. Riley, K.L. March. 2006. Vascularization during tissue injury repair is enhanced by topical treatment with adipose stromal cells. American Heart Association annual meeting.
211. Turk, J.R., S. Korte, K. Kreutzer, M. Linville, C. Murphy, L. Lai, R.S. Prather. 2006. Rickets-like dyschondroplasia in transgenic pigs generated by nuclear transfer. American Association of Veterinary Laboratory Diagnosticians (Minneapolis, 10-12-06).
212. Zhong, Z., M. Katayama, R. Li, L. Lai, Y. Hao, D. Wax, L. Spate, K. Whitworth, Q. Sun, R.S. Prather, H. Schatten. 2006. Distribution and fate of YFP-transfected donor cell mitochondria in cloned porcine embryos. American Society for Cell Biology (Denver).
213. Hao, Y., C. Murphy, L. Spate, D. Wax, Z. Zhong, M. Samuel, N. Mathialagan, R.S. Prather. 2007. Osteopontin improves in vitro development and reduces apoptosis in porcine embryos. Plant and Animal Genome Meetings, San Diego, CA.
214. Prather, R.S., Y. Hao, R. Li, J.W. Ross, J. Whyte. 2007. Heart-healthy pork, and green eggs and ham: genetically modified pigs for medicine and agriculture. Workshop on Advantages of Agriculturally Important Species as Dual-Use Models in Agriculture and Biomedicine. NIH Bethesda, MD April 20, 2007.
215. Whyte, J.J., Y.H. Hao, J.L. Casati, J.R. Turk, M. Samuel, E.B. Rucker III, M.H. Laughlin, R.S. Prather. 2007. Inheritance pattern of porcine endothelial nitric oxide synthase (eNOS) over-expressing transgene in offspring of a cloned transgenic boar bred to wild-type sows. SSR Biol. Reprod. Special Issue P239.
216. Hao, Y., Z. Zhong, L. Spate, C. Murphy, D. Wax, R. Li, M. Samuel, R.S. Prather. 2007. Osteopontin improves in vitro maturation and development of porcine oocytes via activation of the phosphatidylinositol 3-kinase/Akt signaling pathway. SSR Biol. Reprod. Special Issue P112.
217. Yi, Y.J., G. Manandhar, M. Sutovsky, R. Li, V. Jonakova, R. Oko, C.S. Park, R.S. Prather, P. Sutovsky. 2007. Ubiquitin C-Terminal hydrolases-activity is involved in sperm acrosomal function and anti-polyspermy defense. SSR Biol. Reprod. Special Issue P219-220.
218. Whitworth, K.S., R. Li, A. Rieke, D. Wax, W. Spollen, G.K. Springer, P. Sutovsky, J.A. Green, R.S. Prather. 2007. Transcriptional profiling of porcine in vivo, in vitro produced and nuclear transfer derived extraembryonic membranes at day 30 of gestation. SSR Biol. Reprod. Special Issue P194.
219. Park, J., S. L. Smith, L. Lai, M. Samuel, D. Wax, R.S. Prather, X. Yang, X.C. Tian. 2007. Expression profiles of brain, kidney, and lung of cloned pigs. SSR Biol. Reprod. Special Issue P237.
220. Isom, S.C., A.J. Bonk, W. Whitworth, R.S. Prather. 2007. A comparison of global DNA methylation patterns in developmental-competent vs –incompetent nuclear transfer donor cells. SSR Biol. Reprod. Special Issue P228.
221. Rogers, C.S., Y. Hao, T. Rokhjlina, D. Vermeer, Z. Yan, J.F. Engelhardt, R.S. Prather, M.J. Welsh. 2007. Production of CFTR-null heterozygous pigs. North American CF Conference.
222. Prather, R.S., R. Li, J.W. Ross, J.J. Whyte, Y. Hao. 2007. Genetically Modified Swine for Medicine and Agriculture. Frontiers in Transgenesis. Danforth Plant Science Center, St. Louis MO 9/26-27/07.
223. Prather, R.S., R. Li, T. Fu, R. Evans, A. Zhao, Y. Dai. 2007. Heart-healthy pork: hFat-1 transgenic swine. Transgenic Animal Research Conference VI. Granlibakken Conference Center, Tahoe City, CA. Transgenic Research DOI 10.1007/s11248-007-9142-7 16:849-850.
224. Beschorner, W.E., R.S. Prather, L. Lai, P. Kudlacek, K. Harbour-Marik, T. Schieber, K.F. Lechtenberg. 2007. Transgenic pigs producing the suicide gene thymidine kinase in the liver, facilitation of human hepatocytes in the pig liver. International Xenotransplantation Association.
225. Dean, D.A., D.A. Scalise, M. Samuel, R.S. Prather 2007. Extra Cardiac Cellular Matrix For The Delivery Of Stem Cells. Drexel Med. Annual Research Day. Philadelphia, PA Oct 3 2007
226. Nevola, M., D.A. Scalise, R.S. Prather, D.A. Dean. 2007. Extra cardiac cellular matrix for the delivery of stem cells. Discovery Research Day, Drexel University. Oct. 2007.
227. Whitworth, K.M., L.D. Spate, R. Li, A. Rieke, D. Wax, P. Sutovsky, J.A. Green, R.S. Prather. 2008. Transcriptional Profiling of Porcine In Vivo, In Vitro Fertilized and Nuclear Transfer Derived Blastocysts and the Donor Somatic Cell Line. International Embryo Transfer Society annual meeting. Reproduction, Fertility & Development 20:83.
228. Hao, Y., D. Wax, Z. Zhong, C. Murphy, L. Spate, M. Samuel, A. Rieke, J. Li, R.S. Prather. 2008. Pre- and post-implantation development of embryos from cloned porcine skin-originated sphere stem cells. International Embryo Transfer Society annual meeting. Reproduction, Fertility & Development 20:97.
229. Spate, L.D., K. Walker, C. McHughes, R.S. Prather. 2008. Addition of low density lipoprotein to chemically defined protein embryo culture medium can partially replace bovine serum albumin. International Embryo Transfer Society annual meeting. Reproduction, Fertility & Development 20:136-137.
230. McHughes, C.E., G.K. Springer, L. Spate, R. Li, R. Woods, M. Green, S.W. Korte, C.N. Murphy, J.A. Green, R.S. Prather. 2008. Identification and quantification of differentially represented transcripts in preimplantation bovine embryos. International Embryo Transfer Society annual meeting. Reproduction, Fertility & Development 20:169-170.
231. Whitworth, K.M., L.D. Spate, R. Li, A. Rieke, D. Wax, P. Sutovsky, J.A. Green and R.S. Prather. 2008. Transcriptional Profiling of Pig In Vivo and Nuclear Transfer Derived Blastocyst Stage Embryos. Swine in Biomedical Research Meeting, San Diego, CA April 2-3.
232. Prather, R.S.,J.W. Ross, J.J. Whyte, Y. Hao, and Jianguo Zhao. 2008. Biomedical Research Benefits of Genetically Modified Swine. Swine in Biomedical Research Meeting, San Diego, CA April 2-3.
233. Park, J., S. Davis, T. Suteevun, R. Bruno, R. Prather, X. Yang, X.C. Yang. 2008. DNA methylation analyses in lungs of cloned pigs. Biology of Reproduction Special Issue p65.
234. Meyerholz D.K., C.S. Rogers, A. Uc, D.A. Stoltz, P.J. Taft, R.S. Prather, M.J. Welsh. 2008. Pancreatic lesions in a CFTR -/- pig model.Veterinary Pathology 45:764 (#132)
235. Meyerholz D.K., C.S. Rogers, A. Uc, D.A. Stoltz, R.S. Prather, M.J. Welsh. 2008. Porcine cystic fibrosis model: Liver and gallbladder phenotype. Veterinary Pathology 45:764 (#115)
236. Uc, A., D.K. Meyerholz, D.A. Stoltz, C.S. Rogers, R.S. Prather, M.J. Welsh. 2008. Porcine Cystic Fibrosis Model: Liver and Gallbladder Phenotype. (Poster of Distinction at North American Society for Pediatric Gastroenterology, Hepatology and Nutrition (NASPGHAN)/ Children Digestive Health and Nutrition Foundation (CDHNF) meeting, San Diego, CA, November 2008).
237. Uc, A., D.K. Meyerholz, C.S. Rogers, D.A. Stoltz, P.J. Taft, R.S. Prather, M.J. Welsh. 2008. Pancreatic Lesions in a CFTR-/- pig model. (Oral presentation at NASPGHAN-CDHNF meeting, San Diego, CA, November 2008).
238. Zhao, J., J.W. Ross, Y. Hao, D.M. Wax, L.D. Spate, M.S. Samuel, E.M. Walters, A. Rieke, C.N. Murphy, R.S. Prather. 2009. Histone deacetylases inhibitor-Scriptaid benefit the reprogramming of somatic nuclei following nuclear transfer. International Embryo Transfer Society annual meeting (submitted).
239. Hao, Y., J.W. Ross, P. Sutovsky, D. Wax, Z. Zhong, C. Murphy, A. Rieke, M. Samuel, L. Spate, R.S. Prather. 2009. Porcine skin-derived stem cells may be a superior resource to provide donor nuclei for efficient genetic modification of clones. International Embryo Transfer Society annual meeting (submitted).
240. Zhao, M.T., S.C. Isom, J. Zhao, Y. Hao, J.W. Ross, J. Whyte, Y. Zhang, R.S. Prather. 2009. Cooperative expression of pluripotent related genes and neural crest marker genes in porcine GFP-transgenic skin derived progenitors. International Embryo Transfer Society annual meeting (submitted).
241. Spate, L.D., B.K. Bauer, C.N. Murphy, R.S. Prather. 2009. N-methyl-D-aspartic acid can be used to partially replace bovine serum albumin in culture medium. International Embryo Transfer Society annual meeting (submitted).
242. DeMarco, P.J., J.W. Ross, M.A. McCall, K. Narfstrom, R.S. Prather, M. Samuel, E.M. Walters, J. Zhao, H.J. Kaplan. 2009. Development and assessment of a P23H rhodopsin transgenic mini-swine model of retinitis pigmentosa. ARVO

Other

1. Prather, R.S. 1984. Development and evaluation of cryopreservation techniques for bovine embryos. Master's Thesis. Kansas State University.
2. Prather, R.S. 1987. Developmental analysis of murine embryos after chimerization with a highly asynchronous blastomere. PhD Dissertation. University of Wisconsin-Madison.
3. Prather, R.S., M.S. Peters, L.F. Rickords, R.A. Schoenbeck, T.T. Stumpf. 1993. Artificial activation of in vitro matured unfertilized porcine eggs for cloning embryos. National Pork Producers Council Research Investment Report. pp. 219-222.
4. Prather, R.S., A. Rieke, and B.N. Day, 1998. Non-surgical embryo transfer in pigs: Introduction of new genetics with little risk of disease transmission. Embryo Transfer Newsletter September, pp 14-16.

Theses and Dissertations Supervised

Rick A. Schoenbeck. 1992, MS Thesis. Characterization of DNA synthesis and the transition from maternal to embryonic control in the 4-cell porcine embryo.
Mario Mayes. 1994. MS Thesis. Parthenogenetic activation of porcine oocytes by protein kinase inhibitors.
Wendy J. Jolliff. 1997. MS Thesis. Parthenogenetic development of in vitro matured, in vivo cultured porcine oocytes beyond blastocyst
Kristin Whitworth. 1997. MS Thesis. Parthenogenetic activation of porcine oocytes by specific protein kinase inhibitors.
Jae-Hwan Kim. 1999. PhD Dissertation. Involvement of G protein and protein tyrosine kinase signal transduction in pig oocyte activation.
Hyun-Jin Do. 1999. MS Thesis. Expression of pregnancy-associated glycoprotein 1 and 2 genes in in vitro-, in vivo- and parthenogenetically-derived preimplantation pig embryos.
Jon E. Anderson. 2000. PhD Dissertation. Cell cycle regulation in the early porcine embryo.
Nancy T. Ruddock. 2000. PhD Dissertation. Intracellular pH regulation in the early porcine embryo.
David Bart Carter. 2002. MS Thesis. Phenotyping cloned pigs.
Ryan Asa Cabot. 2002. PhD Dissertation. Developmental dynamics of nuclear trafficking in the porcine embryo.
Jennifer Alexis Luth. 2002. MS Thesis. Histone acetylation patterns in preimplantation porcine embryos.
Steven Clay Isom. 2006. PhD Dissertation. The effects of elevated temperature on preimplantation-stage porcine embryos.
Lisa M. Martin. 2006. MS Thesis. Morphologic and histological comparisons between in vivo and nuclear transfer derived porcine embryos.
Aaron J. Bonk. 2007. PhD Dissertation. DNA methylation in the early porcine embryo.
Courtney McHughes. 2007. MS Thesis. Identification and quantification of differentially represented transcripts in preimplantation bovine embryos.
Jin Geol Kim. 2007. MS Thesis. Comparative transcriptional profiling of the uterus according to stage of the estrous cycle and pregnancy status in gilts.

INVITED PRESENTATIONS (Major Presentations Only)

Speaker – American Society for Reproductive Medicine. November 8-12, ’08. San Francisco, CA. “Heart-Healthy Pork, Pig Organs for Humans, Green Eggs and Ham: How Genetically Modified Pigs Can Benefit You”.
Speaker – National Association of State Universities and Land Grant Colleges. April 6, ’08. Columbia, MO. “Life Sciences Research in Animal sciences at the University of Missouri: Genetically Modified Swine for Medicine and Agriculture”.
Speaker – Swine in Biomedical Science. April 2-3, ’08. San Diego, CA. “Genetic Modification in Swine: Current Status”.
Speaker – Transgenic Animal Conference VI. August 12-16, ‘07. Granlibakken Conference Center, Tahoe City, CA. “Heart-healthy pork: hFat-1 transgenic swine”.
Speaker – Advantages of Agriculturally Important Domestic Species as Dual-Use Models in Animal Agriculture and Biomedicine, NIH. April 19-20, ’07. Lister Hill Auditorium, Bethesda, MD. “Genetic Modification of Swine for Medicine and Agriculture”.
Kiracofe Invited Lecture- Kansas State University. March 26-28, ‘07. Manhattan, KS. “Genetic Modification of Swine for Medicine and Agriculture”, “Swine Embryogenesis: Technologies to Improve Reproduction”, “Gene Expression During Swine Embryogenesis: Transcriptional Profiling and Differential Methylation Hybridization”.
Speaker- Utah State University. March 1, ‘07. Logan, UT. “Genetic Modification of Swine for Medicine and Agriculture”.
Walter Randall Lecturer for 2006. American Physiological Society, Experimental Biology Annual Meeting, April 1-5, ’06. San Francisco, CA. “Transgenic animals for medicine and agriculture: do the ends justify the means?”
Speaker- Oklahoma State University. March 9 & 10, ’06. Stillwater, OK. “Gene Expression During Pig Embryogenesis: Transcriptional profiling and differential methylation hybridization”, and “Genetic Modification of Swine for Medicine and Agriculture”.
Speaker – University of Wisconsin-Madison. Dec. 8 & 9, ’05. Madison, WI. Endocrinology, Reproductive Physiology Training Program. “Gene Expression During Pig Embryogenesis”.
Speaker – American Association of Lab Animal Scientists, Annual Meeting November 6-10, ’05. St. Louis, MO. “Transgenic Approaches to Develop Swine Models”.
Speaker – Institute for Theological Encounter with Science and Technology. Biological Advance Patenting and the Law. October 14-16, ‘05. Belleville, IL. “Discussion of Reproductive Biotechnologies for the Institute for Theological Encounter with Science and Technology”.
Speaker- Missouri State University. Sept. 21 & 22. ‘05. Springfield, MO. “Genetic Modification of Swine for Medicine and Agriculture” and “Swine and Bovine Genomics Research with a Focus on Female Reproduction”
Speaker- 7th International Conference on Pig Reproduction. June 14, ‘05. Rolduc Abbey, The Netherlands. “Pig Reproduction: A Look Back, and Ahead.”
Speaker- University of Iowa. April 18, ’05. Univ. of Iowa Gene Therapy Center Annual Retreat. Iowa City, IA. “Genetic Modification of Swine for Medicine and Agriculture.”
Speaker- Iowa State University. March 23-24, ’05. Ames, IA. “Genetic Modification of Swine for Medicine and Agriculture.”
Speaker- University of Connecticut-Storrs. February 24-25, ’05. Storrs, CT. “Genetic Modification of Swine for Medicine and Agriculture” & “Gene Expression During Pig Embryogenesis as Determined by Using a Pig Reproductive Tissue-Specific Microarray.”
Speaker- Swine in Biomedical Research, Chicago IL. January 27, ’05. “Perspectives on Porcine Transgenesis and Cloning.”
Speaker- Plant & Animal Genome-XIII Swine Workshop Program, San Diego, CA. January 15, ’05. “Gene Expression During Pig Embryogenesis as Determined by Using a Pig Reproductive Tissue-Specific Microarray.”
Speaker- Monsanto Company, Inc. Chesterfield, MO. November 19, ‘04. “Gene Expression During Pig Embryogenesis as Determined by a Pig Reproductive Tissue-Specific Microarray”.
Speaker- Workshop on Advantages of Agriculturally Important Domestic Species as Biomedical Models. Michigan State University, East Lansing, MI. October 29-31, ’04. “Swine as Biomedical Models”.
Speaker- American Embryo Transfer Association. Tampa, FL. October 14-16, ‘04. “Genetic Modification of Swine for Medicine and Agriculture”.
Speaker- Society for the Study of Reproduction- Annual Meeting. Vancouver, BC, Canada. Aug. 1, ’04. “Genetic Modification of Pigs for Medicine and Agriculture”
Speaker- American Association of Animal Scientists- Annual Meeting, St. Louis, MO. July 26, ’04. “Swine and Bovine Genomics Research with a Focus on Female Reproduction”.
Speaker- Gordon Research Conference – Reproductive Tract Biology, New London, CT. June 6-11, ’04. “Development, ‘or lack thereof’, of nuclear transfer-derived embryos”.
Speaker- Washington University, St. Louis, MO, December 17, ’03. “Genetic Modification of Swine for Medicine and Agriculture”.
Speaker- Korean Conference on Innovative Science and Technology. Jeju Island, South Korea. November 12-14, ’03. “Genetic Modification of Swine for Medicine and Agriculture”.
Speaker- Food & Drug Administration. October 7, 2003, Rockville, MD. “Transgenic Pigs for Medicine and Agriculture”.
Speaker- International Conference on Transgenic Animal Production. August 10-14, ’03. Tahoe, CA. “Transgenic Pigs for Medicine and Agriculture”.
Speaker- American Meat Science Association, June 18, ’03. Columbia, MO. “Use of Cloning and Transgenesis in Pigs”
Speaker- Nebraska Physiological Society, May 23, ’03. Lincoln, NB. “Specific Genetic Modifications to Swine: Application to Medicine and Agriculture”.
Distinguished Lecturer- University of Florida’s Animal Molecular and Cell Biology Graduate Program- 1st Annual Research Symposium. May 2-3, ’03. The Whitney Laboratory, Marineland, FL. “Swine and Bovine Genomics Research with a Focus on Female Reproduction”
Speaker- University of Florida, May 2, ’03. Gainesville, FL. “Transgenic Pigs for Medicine and Agriculture”
Speaker- Genetics at Science City, Union Station, Kansas City, MO. April 28, ’03. “Transgenic Pigs for Medicine and Agriculture”
Grand Rounds-Ob/Gyn, Case Western Reserve University, MacDonald Women’s Hospital. March 5, ’03. Cleveland, OH. “Transgenic Pigs for Medicine and Agriculture”
Speaker- International Embryo Transfer Society, January 13, ’03. Auckland, New Zealand. “Transgenic Pigs for BioMedicine and Agriculture”.
Speaker – Linda Hall Library. December 9, ’02. Kansas City, MO. “Swine with Specific Genetic Modifications: Cloning & Xenotransplantation”.
Speaker- American Heart Association Annual Meeting, November 17-20, ’02. Chicago, IL. “Xenotransplantation: Genetic Modification to Overcome the Barriers”.
Speaker- Minority Donor Awareness Society, October 4, ’02. Kansas City, MO. “Xenotransplantation- Genetic Modification in Swine”.
Speaker- Iowa State University. September 17, ’02. Ames, IA. “Transgenic Pigs for Biomedicine and Agriculture”.
Speaker- Monsanto Company. September 4, ’02. Chesterfield, MO. “Transgenic Pigs for Biomedicine and Agriculture”.
Speaker- First International Workshop on Embryo Genomics in Farm Animals, July 20, ’02. Quebec City, Quebec, Canada. “Large Scale EST Project to Discover Genes of Importance to Pig Reproduction”.
Speaker- Harvard Medical School. Massachusetts General Hospital/Transplantation Biology Research Center/Immerge BioTherapeutics, May 24, ’02. Charlestown, MA. “Transgenic Pigs for Biomedicine and Agriculture”.
Speaker- University of Illinois, May 1, ’02. “Transgenic Pigs for Biomedicine and Agriculture”.
Speaker- Secretary’s Advisory Committee on Xenotransplantation, March 11, ’02. National Institutes of Health, Bethesda, MD. “Transgenic Pigs for Biomedicine and Agriculture”.
Speaker- Mo-Kan Reproductive Biology Workshop, November 3, ’01. Columbia, MO. “Nuclear Transfer in Swine- Cloning & Transgenesis: Theory, Practice & Potential”.
Visiting Expert Program. The Ohio State University. October 2-4, ‘01. Wooster and Columbus, OH. “Nuclear Transfer in Swine- Cloning & Transgenesis: Theory, Practice & Potential”.
Speaker- International Animal Agriculture and Food Science Conference, July 25, ’01. Indianapolis, IN, “Preserving/Conserving Germplasm by Incorporating Embryo-Related Technologies”.
Speaker- 6th International Conference on Pig Reproduction. June 3-6, ’01. Columbia, MO. “Cellular Mechanisms of Fertilization and Parthenogenesis”.
Speaker- University of Nebraska-Lincoln. April 11, ’01. Lincoln, NB. “Development of Embryo-based Bio-technological Advances in Swine Embryogenesis”.
Speaker- Michigan State University, March 28, ’01. East Lansing, MI. “Development of Embryo-based Bio-technological Advances in Swine Embryogenesis”.
Speaker – International Congress on Animal Reproduction. July 2-4, ’00. Stockholm, Sweden. “Parthenogenetic Activation of Swine Oocytes”.
Speaker- Nuclear Transfer and Transgenesis. June 18-21, ‘00. Park City, Utah. “Changes in the Structure of Nuclei after Transfer to Oocytes”.
Speaker- Banbury Conference- Mammalian Cloning: Can It Be Made More Efficient? March 12-15, ’00. Cold Spring Harbor, NY. “Nuclear Transfer in Swine”.
Speaker- Missouri Veterinary Medical Association. January 21, ’00. Tan Tara, Missouri. “Development of Embryo-Technologies in Swine”
Speaker – Purdue University. October 21, ’99. West Lafayette, Indiana. “Differentiation of Swine Embryos and Progress in Cloning/Transgenesis”.
Speaker – Institute for Theological Encounter with Science and Technology. “The Genome: Plant, Animal, Human”. August 1-5, ‘99. Loyola University of Chicago, Chicago, IL “Genetic Modification of Domestic Animals”.
Speaker/Panelist – Tau Beta Pi 30th Annual Paul D. Scholz Symposium. “Human Cloning and Genetic Engineering”. April 27, ‘99, University of Iowa, Iowa City, IA. “Development of the Cloning/Nuclear Transfer/Transgenic Technology”.
Speaker - International Embryo Transfer Society-Satellite Session. January 13, ‘99. Chateau Mont Sainte-Anne. Quebec, Canada. “Development of the techniques for nuclear transfer in pigs”.
Speaker – The American Society of Human Genetics. October 28, ‘98. “Transgenic animals by nuclear transfer (cloning): theory, practice & potential”.
Speaker – Association of University Technology Managers, Inc. September 14, ‘98. Kansas City, MO. “The Patent Frenzy – A Few Case Studies”.
Speaker – Utah State University. September 8, ‘98 Logan, UT. “Development of Embryo Technologies in Pigs”.
Speaker- Nuclear Transfer and Embryonic Stem Cells. April 15-17, ‘98. Monash University, Australia. “Strategies for Activating Nuclear Transfer Oocytes”.
Speaker- International Embryo Transfer Society. . January 18, ‘98. Boston, MA. “Practical Aspects of In Vitro Production of Pig Embryos”.
Speaker & Panelist- Cloning and Biotechnology: Opportunities, Challenges and Choices. November 13, ‘97. University of Nebraska-Lincoln. “Cloning Mammals: Theory, Practice & Potential”.
Speaker- Mammalian Cloning: Implications for Science and Society. June 26, ‘97. Crystal Gateway Marriott Hotel, Arlington, VA. “Nuclear and Cytoplasmic Modifications After Nuclear Transfer”.
Speaker- Roslin Institute, Roslin, Scotland. May 29, ‘97. “Activation of Pig Oocytes”.
Speaker- Lincoln University, Jefferson City, MO. February 25, ‘97. "Chimera Production in Swine, and the Potential for Xenographs."
Speaker- Korean Society of Animal Reproduction, Seoul, South Korea. February 14, ’97. "Artificial Activation of Pig Oocytes Arrested at Meiotic Metaphase II".
Speaker- Korea Research Institute Bioscience and Biotechnology, TaeJeon, South Korea. February 13,’97. "Development and Applications of Embryo Technologies in Pigs".
Speaker- 4th Midwestern Meeting on Conceptus and Uterine Development, Iowa State University, Ames, IA. March 21, ‘94. "Artificial Oocyte Activation in Pigs".
Speaker- Louisiana State University, Baton Rouge, LA. November 5, ’93. "When is Development of the Domestic Pig Conceptus Dependent Upon New Transcription?"
Speaker - International Society for Heart Research, American Section, ‘93 annual meeting. Columbia, MO. June 20-24,’93. "Strategies for Producing Large and Small Transgenic Animals".
Speaker- 4th International Conference on Pig Reproduction, Columbia, MO. May 23-26, ‘93. "Nuclear Control of Preimplantation Development in the Pig".
Speaker- University of Montreal, CRRA, Saint-Hyacinthe, Quebec, Canada. March 23, ‘93. "The Transition from Maternal Control of Development to Zygotic Control of Development in the Domestic Pig".
Speaker- Biotransplant, Inc. & Harvard Medical School, Boston, MA. November 17, ’92. "Cloning Pig Embryos".
Speaker - NCR 150, Animal Biotechnology: Technology Transfer and Industrial Needs, National Livestock & Meat Board, Chicago, IL. May 17-19, ‘92. "Nuclear Transplantation as a Method of Producing Genetically Identical Livestock".
Speaker- Chicago College of Osteopathic Medicine, Downers Grove, IL. March 30,’92. "Nuclear Transfer for Cloning Domestic Animals: Nuclear Remodeling and Reprogramming".
Speaker - International Symposium on: 'Cloning Mammals by Nuclear Transplantation', Fort Collins, CO, January 15, ‘92. "Nuclear Modifications after Nuclear Transfer".
Speaker - Kansas State University, Departmental Seminar & Guest Lecturer, April 1-3,’91, Manhattan, KS. "The Transition From Maternal-to-Zygotic Control of Development", "Cloning Livestock Embryos by Nuclear Transfer", "Nuclear Remodeling & Reprogramming After Nuclear Transfer".
Speaker- Gordon Research Conference, Quantitative Genetics and Biotechnology, January 11, ‘91, Oxnard, CA. "Nuclear Transplantation in Animals".
Speaker - Mo-Kan Reproductive Biology Workshop, June 16, ‘90, University of Missouri-Columbia. "Cloning Embryos by Nuclear Transfer".
Speaker - University of Nebraska, Biotechnology Seminar Series, March 7, ‘90, Lincoln, NE. "Nuclear Transfer and Nuclear Reprogramming in Domestic Animals".
Speaker - Banbury Conference - Applications of Basic Research in Development. October 15-18,’89, Cold Spring Harbor, NY. "Cloning by Nuclear Transfer in Laboratory and Domestic Animals".
Speaker- 2nd International Symposium on Genetic Engineering of Animals, June 25-28, ‘89. Cornell University, Ithaca, NY. "Reprogramming and Cloning the Entire Genome".
Speaker- 3rd International Conference on Pig Reproduction. April 11-14, ‘89. University of Nottingham, U.K. "Cloning of Embryos".
Faculty - The American Fertility Society, Regional Postgraduate Course, Hands on IVF, Cryopreservation and Micromanipulation. April 25-29, ‘88, Madison, Wl. "Nuclear Transfer in Early Mammalian Embryos".
Speaker- Application of Egg- and Embryo-Technologies to Domestic Animals. Department of Obstetrics and Gynecology, January ‘87. The Royale Veterinary and Agricultural University, Copenhagen, Denmark. "Nuclear Transplantation in Domestic Animal Embryos".

University of Missouri

Director: Transgenic Animal Facility, ‘90 - ‘94. This was a service facility to produce transgenic mice for on-campus investigators.

Supervisor: Unit D facilities of ASRC, Sept. ‘94 - Present. These are the large animal and surgery units in the Animal Science Research Center.

Committees,
Campus
Special Advisory Group to the Chancellor, Jan. ’05-Present.
DNA Core Advisory Committee, April 7, ‘99-Present.
Molecular Biology Core Advisory Committee, Aug. ’04-Feb. ’07.
Provost’s Life Sciences Task Force, Dec. ’00-Dec. ‘04.
Transgenic Animal Core Facility Oversight Committee, ‘99-‘02.
Life Sciences Mission Enhancement Planning Committee, April ‘98-Aug. ‘99.
Advisory Committee for Office of Research, Aug. ‘98- Aug. ’01.
Electron Microscopy Core Facility Oversight Committee, Nov. ‘96-’97 & ‘98-Jan. ‘99.
Animal Care and Use Committee, Jan.’97-April ‘98.

College
CAFNR Promotion and Tenure Committee. Sept. ’02-Aug. ’04.

Departmental
Promotion and Tenure Committee, July ‘96-’99.
Swine Farm Operations Committee, June ‘89-present.
Vehicle Committee, Chairman, Jan.’90-’95.
GCI/RIF/Research Advisory Committee, Aug. ‘90-’96.
Research Advisory Committee: GCI/RIF/GIF, Chairman, ‘96-’98; ’02-‘04.
Unit D1-3 Space Committee, Oct. ‘91-Sept. ‘94.
Unit D1-3 Space Committee, Chairman, Sept. ‘94-present.
ASRC Space Committee, Sept. ‘94-present.
Faculty Awards Committee, Oct.’94-’97.
Policy Committee on Patents, etc., Aug 7, ‘95.
Policy Committee on Support of Farms, Aug 8, ‘95.

Food for the 21st Century, Animal Reproduction Cluster
Fellowships and Other Awards, Chairman ’00-Present.
Pilot Projects/Special Equipment, Jan. ‘91-’92.
Pilot Projects/Special Equipment, Chairman ‘94.
Invited Speakers, Jan.’91-’92.
Invited Speakers, Chairman, Aug. ‘94-Dec. ‘94.
Publications, Chairman, Oct. ‘92-’94.
Publications, ‘95-‘05.
Support Facilities, Oct.’92-’94.
Fellowships, Chairman ‘95-’98.
Transgenic Lab,’95-’98.

Search Committees

F21C Reproductive Biology Cluster, One Position, ‘92-’93, Filled by Matt Lucy

F21C Reproductive Biology Cluster, Two Positions, ‘96-‘98, Filled By Guang Zhao & the other position was re-advertised.
Dean of the College of Veterinary Medicine, ‘98-’99- Filled by Joe Kornegay.
McKee Endowed Professor, Department of Veterinary Pathobiology, ’03-’04, Filled by John Critser.
Gamete & Embryo Cryobiology, Department of Veterinary Pathobiology, ’03-’04, Filled by Yuksel Agca.
F21C Reproductive Biology Cluster, Two Positions. ’04-‘05. Committee Chair. Filled by Roccio Rivera and Kevin Wells.
ObGyn Faculty, Three Positions. Filled by Gary Clark, Bret Barrier and Susan Nagel. ’04-‘05.
Chair of Veterinary Pathobiology, Department of Veterinary Pathobiology, ’05. Filled by George Stewart.
F21C Reproductive Biology Cluster, One Position, ’08-Current.

Teaching

413 Reproductive Biology Seminar W90: Coordinated Graduate Seminar. 1 hr
433 Gamete & Embryo Biology F90: Developed and Team Taught This Graduate Level Class. 2 hr
433 Gamete & Embryo Biology F92: Team Taught This Graduate Level Class. 2 hr
Biology of Gestation W93: Developed & Team Taught This Graduate Level Class.2 hr
433 Gamete & Embryo Biology F94: Team Taught This Graduate Level Class. 2 hr
400 Experimental Surgery F94: Coordinated this class taught by Dr. Youngquist. 1 hr
384 Al & Reproductive Management F95: Revamped & Team Taught This Upper Level Undergraduate Class. 3 hr
400 Experimental Surgery F95: Coordinated this class taught by Dr. Youngquist.1 hr
400 Experimental Surgery F96: Coordinated this class taught by Dr. Youngquist.1 hr
400 Gametes, Embryos & Gestation F96 (AS433 + Gestation): Team taught this Graduate Level Class. 3 hr
384 AI & Reproductive Management F96: Taught this undergraduate level class. 3 hr
301 Genetics W97: Taught the first 5 lectures for Dr. Lamberson. 3 hr.
384 AI & Reproductive Management F97: Team Taught This Upper Level Undergraduate Class. 3 hr
400 Experimental Surgery F97: Coordinated this class taught by Dr. Youngquist.1 hr
301 Genetics W98: Taught the first 5 lectures for Dr. Lamberson. 3 hr.
384 AI & Reproductive Management F98: Team Taught This Upper Level Undergraduate Class. 3 hr
400 Experimental Surgery F98: Coordinated this class taught by Dr. Youngquist.1 hr
384 AI & Reproductive Management F99: Team Taught This Upper Level Undergraduate Class. 3 hr
400 Experimental Surgery F99: Coordinated this class taught by Dr. Youngquist.1 hr
384 AI & Reproductive Management F00: Team Taught This Upper Level Undergraduate Class. 3 hr
400 Experimental Surgery F00: Coordinated this class taught by Dr. Youngquist.1 hr
440 Gamete & Embryo Biology F00: Taught This Graduate Level Class. 2 hr
384 AI & Reproductive Management F01: Team Taught This Upper Level Undergraduate Class. 3 hr
384 AI & Reproductive Management F02: Team Taught This Upper Level Undergraduate Class. 3 hr
213 Genetics W03: Taught the first 6 lectures of this course for Dr. Lamberson. 3 hr.
384 AI & Reproductive Management F03: Team Taught This Upper Level Undergraduate Class. 3 hr
213 Genetics W04: Taught the first 5 lectures of this course for Dr. Lamberson. 3 hr.
4384 AI & Reproductive Management F04: Team Taught This Upper Level Undergraduate Class. 3 hr
3213 Genetics W05: Taught the first 5 lectures of this course for Dr. Lamberson. 3 hr
4384 AI & Reproductive Management F05: Team Taught This Upper Level Undergraduate Class. 3 hr
3213 Genetics W06: Taught the first 5 lectures of this course for Dr. Lamberson. 3 hr
4384 AI & Reproductive Management F06: Team Taught This Upper Level Undergraduate Class. 3 hr
3213 Genetics W07: Taught the first 5 lectures of this course for Dr. Lamberson. 3 hr
4384 AI & Reproductive Management F07: Team Taught This Upper Level Undergraduate Class. 3 hr
3213 Genetics W08: Mentored Erin Sellner to teach the first 5 lectures of this course for Dr. Lamberson. 3 hr
4384 AI & Reproductive Management F08: Team Taught This Upper Level Undergraduate Class. 3 hr

Graduate Faculty,
Summer 1989 to present.

Doctoral Faculty,
January 1, 1991 to present.

Postdoctoral Students, Current Position
Lee F. Rickords 1/91-9/92. Assistant Professor: Utah State University
Todd T. Stumpf 1/91-1/92. Research Coordinator: Continental Grain
Jiewen Li 10/93-12/96. Postdoc with Alan Bradley, Baylor College of Medicine
Zoltan Macháty 10/93-4/97. Assistant Professor- Purdue University
Yong-Mahn Han 3/97-9/98. Research Scientist: KIST, Tae-Jeon, Korea
Tao Tao 6/97-4/99. Director Clinique de Fertilite, Moncton, NB, Canada.
Jae-Hwan Kim 5/99-8/99 Assistant Professor, Pochon CHA University, Korea Birgit Kühholzer 10/98-10/00 Homemaker.
Zhi-Xin Wei 3/99-5/01 Postdoc, University of Chicago
Kwang-Wook Park 4/99-4/02 Faculty, Sunchon National University, Korea
Liangxue Lai 12/98-4/02 Investigator, Guangzhou Institute of Biomedicine and Health, China.
Gi-Sun Im 5/02-12/03 Korea National Livestock Research Institute
Zhonghua Liu 8/02-6/04 Associate Professor- Northeast Ag. Univ., Harbin China
Hwan-Yul Yong 12/03-11/05 Postdoctoral Fellow: Univ. Missouri- Mike Roberts
Rongfeng Li 1/05-12/07 Associate Professor- Inner Mongolia University, China.
Jason Ross 12/06-7/08 Assistant Professor, Department of Animal Science, Iowa State University
S. Clay Isom 9/06-Present
Jeff Whyte 9/06-Present
Jianguo Zhao 9/07-Present

Research Assistant Professor,

Zoltan Machaty 4/97-8/00. Assistant Professor, Department of Animal Science, Purdue University

Liangxue Lai 4/02-12/06. Investigator, Guangzhou Institute of Biomedicine and Health, China.

Visiting Scientists, Home Position

Seung-Ju Moon 8/94-7/95 Chonnam National University, Korea
Prudence Aquino-Aquino 10/97 St. Luke’s Medical Center, Quezon City, Philippines
Gabor Vajta 11/97 Danish Institute of Agricultural Sciences, Tjele, Denmark
Jorge Piedrahita 12/97 Texas A&M, College Station, TX
James L. Sartin 9/99 College of Veterinary Medicine, Auburn University
Richard Taylor 10/99 E.A.R.T.H. Costa Rica
Jin-Ki Park 7/99-7/00 Korea NLRI-RDA
Hee-Tae Cheong 7/00-8/01 Kangwon National University, Korea
Yan-Hong Hao 10/01-12/02 Northeast Ag. Univ. Harbin, China
Clifton Murphy 1/95-Present College of Veterinary Medicine, UMC
Gabor Vajta 5/03 Danish Institute of Agricultural Sciences, Tjele, Denmark
Yan-Hong Hao 6/03-1/09 Northeast Ag. Univ. Harbin, China
Yi Tan 1/06-4/06 Chongqing University of Medical Sci., China
Mingtao Zhao 11/07-Present Institute of Biotechnology, Northwest A&F University, Yangling, Shannxi, 712100, P. R. China.

Graduate Student Honors.

Rick Schoenbeck *Finalist Student Poster Competition, International Embryo Transfer Society ’93.
Jon Anderson *Awarded 2nd place in the Research and Creative Activity Forum ‘99, University of Missouri-Columbia.
*Awarded the Donald K. Anderson Graduate Student Teaching Award ‘98, University of Missouri-Columbia.
*Awarded the Superior Graduate Achievement Award ‘97. University of Missouri-Columbia.
Nancy Ruddock * Animal Science Graduate Student Association- Social Committee, ’97-’99, President ’99-‘00
*Awarded a Brown Fellowship and spent a month in the laboratory of Dr. Alan Trounson, Monash medical Center, Australia.
David Bart Carter * Awarded the Donald K. Anderson Graduate Student Teaching Award ‘01, University of Missouri-Columbia.
Ryan Asa Cabot * College of Agriculture, ’02. Food and Natural Resources, Research Expo XI poster session winner.
Jin-Geol Kim * Neal A. Jorgensen Genome Travel Grant- to Present data at the Plant and Animal Genome Meeting, San Diego, CA. ’05.
Aaron Bonk *2nd Place Poster Competition, Division of Animal Science- Grad Forum June 30, ’05.
Aaron Bonk *Finalist Student Poster Competition, International Embryo Transfer Society, Orlando, FL. ’06.
Kristin Whitworth *Finalist Student Poster Competition, International Embryo Transfer Society, Denver, CO. ’08. Named a Runner-Up, i.e. 2nd place.
Kristin Whitworth *First Place. Graduate Student Forum, ’08. Oral Presentations, Division of Animal Sciences, University of Missouri.

Graduate Advising,

As Major Professor (10 MS and 6 PhDs graduated)
MS Rick A. Schoenbeck1992 Director Reproduction Resources, Hebron, IL
MS Mario Mayes 1994 Research Scientist, University of Laval, Quebec, Canada
MS Wendy Jolliff 1997 Homemaker
MS Kristin Whitworth 1997 Sr. Research Specialist/PhD student at the University of Missouri
PhD Jae-Hwan Kim 1999 Assistant Professor, Pochon CHA University, Korea
MS Hyun-Jin Do 1999 Research Specialist, Pochon CHA University, Korea
PhD Jon Anderson 2000 Embryo Technology Research Scientist, Sygen
PhD Nancy Ruddock 2000 Postdoc in Australia, Melbourne (Alan Trounson)
MS Bart Carter 2002 Director, Office of Animal Resources, Kansas State University
PhD Ryan Cabot 2002 Assistant Professor, Department of Animal Science, Purdue University
MS Jennifer Luth 2002 Postdoc at Washington Univ. St. Louis, MO
MS Lisa Overman-
Martin 2006 Veterinarian, North Carolina
PhD S. Clay Isom 2006 Postdoc at the University of Missouri
PhD Aaron Bonk 2007 Technical Service Rep. with Promega.
MS Jin-Geol Kim 2007 Research Specialist, University of Missouri
MS Courtney McHughes 2007 Sales consultant, Mt. Pleasant, TX
MS Scott Korte Current
PhD Kristin Whitworth Current
MS Bethany Bauer Current
MS Kyle Dobbs Current

As Committee Member

MS Steve Terlouw 1989
MS Shyh-Jye Lee 1992
PhD Gregg Eckardt 1992
PhD Doug Leaman 1993
PhD Stacey Hamilton 1993
PhD Steve Terlouw 1993
PhD Julie Galvin 1993
PhD Tim Safranski 1994
PhD Melody Stallings-Mann 1995
PhD Rongti Liang 1995
MS Nicole M. Ferguson-Kohout 1998
MS Christopher Hueser 1999
PhD Will Rieke 2000
MS George Perry 2000
PhD Melissa Larson 2000
MS Jennifer Antelman 2007
PhD Steve Mullen 2007
MS Madhavi Kommareddy 2008
PhD Bhanu Prakash Telugu 2008
PhD Elena Kocher Current
PhD Steve Smith Current
PhD Shawn Zimmerman Current